Staining

Question 1

as a 0.01% alcoholic solution. can be used as an alternativeto basic fuchsin in Schiff’s reagent, for the Feulgen technique of acid hydrolysis.

Answer 1

Acriflavine

Question 2

is the most commonlv used fluorochrome to demonstrate
Acriflavine, DNA and RNA in fresh or tixed tissues, combining with nucleIc acids in cells by salt linkages and cohesion. DNA emits a yellow-green fluorescence while RNA is stained brick to orange-red This is used for screening of cervical smears for cancer cells.

Answer 2

Acridine Orange

Question 3

conjugates absorb maximally in green light, exhibiting an orange-red emission

Answer 3

Rhodamine

Question 4

Fluorochromes are fluorescent dyes which emit light or visible radiation energy when excited by light of shorter wave length, either visible or ultraviolet.

Answer 4

Fluorescent Staining for DNA and RNA

Question 5

differential staining reaction respectively.

Answer 5

Methyl Green-Pyronin
method for RNA and DNA

Question 6

is a staining technique used to identify chromosomal material
DNA Methyl Green-Pyronin
or DNA in cell specimens.

Answer 6

Feulgen Staining for Nuclear DNA

Question 7

a stain that colors fat droplets black and is the most sensitive of the oil soluble dyes.

Answer 7

Sudan Black

Question 8

It is recommended for staining triglycerides (neutral lipids). giving them a deep and intense red stain.

Answer 8

Sudan IV (Scharlach R)

Question 9

fat stain for central nervous system tissues. giving a less deer and lighter orange stain compared to the darker staining Sudan IV

Answer 9

Sudan III

Question 10

causes blood cells to exhibit four major staining properties that allow the cell.

Answer 10

Wright Stain

Question 11

is used with osmic acid to fix and stain blood and glandular tissues.

Answer 11

Rhodamine B

Question 12

has sometimes been used for staining erythrocytes is weakly basic dye used as a contrast stain for staining ascaris eggs and erythrocytes, and as a bacterial spore stain; it is also used both as a decolorizerand as a counterstain

Answer 12

Malachite Green

Question 13

is used as a chromatin stain for fresh materials in smear
preparations.

Answer 13

Carmine stains

Question 14

is used as a contrast stain for Gram’s techniaue. in acid fast and Papanicolau method, and for staining diphtheria organisms

Answer 14

Bismarck Brown

Question 15

one of the most valuable stains used for ditterentially staining connective tissues and cvtoplasm
It Is commonly used as a background stain because it gives a pleasing and colortul contrast to nuclear stains

Answer 15

Eosin

Question 16

It is a popular cytological stain, especially for the study of mitosis

Answer 16

Heidenhain’s Hematoxvlin

Question 17

For mitochondria staining

Answer 17

Regaud’s hematoxylin

Question 18

It is used as a nuclear counterstain to demonstrate the presence of cytoplasmic glycogen by special stain.

Answer 18

Mayer’s hematoxylin

Question 19

Used for diagnosing Alzheimer’s Disease to show neuritic components of plaques and tangles

Answer 19

Bielschowsky Silver Stain

Question 20

Used for the detection of amyloidal plaques in brain

Answer 20

Congo red

Question 21

Useful in identifying cell bodies of neurons in tissue sections

Answer 21

Cresyl Violet Stain

Question 22

Used to stain abnormal neuroglia (reactive astrocytosis)

Answer 22

Phosphotungstic Acid-Hematoxylin Stain (PTAH)

Question 23

Used for staining structures containing a high proportion of carbohydrate macromolecules glycogen and giycoprotein, basement membranes, collagen and primary cell types

Answer 23

Periodic Acid-Schiff (PAS) Stain

Question 24

Used for the identification of chromosomal material or
deoxyribonucieic acid DNA In paraffin-embedded tissue or cell
specimens)

Answer 24

Feulgen stain

Question 25

Demonstrates hemosiderin in bone marrow macrophages and within erythroblasts

Answer 25

Perls’ Prussian
Blue stain

Question 26

Used for staining lipids in frozen sections and some lipoproteins
on parattin sections

Answer 26

Oil red O

Question 27

Detects encapsulated veast-like rungus cryptococcus neofarmans

Answer 27

Mayer’s Mucicarmine Stain

Question 28

Used for distinguishing collagen and smooth muscle fibers;

Answer 28

Gomori’s One-Step Trichrome Stain

Question 29

Used in identifying mucins and glycosaminoglycans. At pH 2.5,
It stains sulphated and nonsulphated acidic carbohydrates. At pH 1.0, onlv sulphated carbohydrates are stained

Answer 29

Alcian Blue

Question 30

Used in hematology, e.g., for the detection of erythroidcolonies, binucleate normoblast, megaloblasts, mast cells, etc. it is also used for chromosome staining;

Answer 30

Giemsa Stain