Method Of Streaking

Question 1

process whereby tissue components are made visible in microscopic sections by direct interaction with a dye or staining solution.

Answer 1

Staining

Question 2

The process of giving color to the sections by using aqueous or alcoholic dye solutions. In simple staining only one dye isused, which is washed away after 30-60 seconds, prior to drying and examination.

Answer 2

Direct staining

Question 3

The process whereby the action of the dye is intensified by adding another agent or a MORDANT which serves as a link or bridge between the tissue and the dye, to make the staining reaction possible.

Answer 3

Indirect staining

Question 4

It does not participate in the staining reaction, but merely accelerates the reaction.

Answer 4

Accentuator

Question 5

The process whereby tissue elements are stained in definite sequence, and the staining solution is applied for specific periods of timeor until the desired intensity of coloring of the different tissue elements is attained.

Answer 5

Progressive staining

Question 6

With this technique, the tissue is first overstained to obliterate the cellular details, and the excess stain is removed or decolorized front, unwanted parts of the tissue, until the desired intensity of color is obtained.

Answer 6

Regressive staining

Question 7

is the selective removal of excess stain from the tissue during regressive staining in order that a specific substance may be stained distinctly from the surrounding tissues.

Answer 7

Differentiation (Decolorization)

Question 8

uses more than one chemical stain to better differentiate between various microorganisms or structures/cellular components of a single organism

Answer 8

Differential staining

Question 9

technique entails the use of specific dyes which differentiate particular substances by staining them with a color that is different from that of the stain itself (metachromasia).

Answer 9

Metachromatic staining

Question 10

is a process where specific tissue elements aredemonstrated, not by stains, but by colorless solutions of metallic salts which arether by reduced by the tissue, producing an opaque, usually black deposit on thesurface of the tissue or bacteria.

Answer 10

Metallic impregnation

Question 11

is the selective staining of living cell constituents, demonstrating cytoplasmic structures by phagocytosis of the dye particle cytoplasmic phagocytosis), or by staining of pre-existing cellular components

Answer 11

Vital staining

Question 12

done by injecting the dye into any part ofthe animal body either intravenous, intraperitoneal or subcutaneous), producing specific coloration of certain cells, particularly those of the reticulo-endothelialsystem.

Answer 12

Intravital staining

Question 13

a method of staining used in microscopy to examine living cells that have been removed from an organism

Answer 13

Supravital staining

Question 14

is the corner stone of tissue-based diagnosis. The process stains thin tissue sections so that pathologists can visualize tissue morphology. The process uses a hematoxylin dye to stain cell nuclei (and other parts) blue and an eosin dye to stain other structures pink or red

Answer 14

Hematoxylin and Eosin (H&E) staining

Question 15

are substances that combine with the tissue and the staining solution, forming a “bridge” that allows staining reaction tо takе place

Answer 15

Mordants

Question 16

are substances with definite atomic groupings and are capable of producing visible colors.

Answer 16

Chromophores

Question 17

where the active coloring substance is found in the acid component, and the inactive base, e.g. acid fuchsin,

Answer 17

Acid dyes

Question 18

where the active coloring substance is found in a basic component that combines with the acid radical (usually taken from sulfuric, acetic or hydrochloric acid). An example of a basic nuclear stain is methylene blue, which may be usedboth as an indicator and as a dye.

Answer 18

Basic dyes

Question 19

are formed by combining aqueous solutions of acid and basic dyes, capable of staining cytoplasm and nucleus simultaneously and differentially. Examples are Romanowsky dyes used in hematology. Giemsa’s stain, and Irishman’s stain for leukocyte differentiation.

Answer 19

Neutral dyes