Method Of Streaking Flashcards
process whereby tissue components are made visible in microscopic sections by direct interaction with a dye or staining solution.
Staining
The process of giving color to the sections by using aqueous or alcoholic dye solutions. In simple staining only one dye isused, which is washed away after 30-60 seconds, prior to drying and examination.
Direct staining
The process whereby the action of the dye is intensified by adding another agent or a MORDANT which serves as a link or bridge between the tissue and the dye, to make the staining reaction possible.
Indirect staining
It does not participate in the staining reaction, but merely accelerates the reaction.
Accentuator
The process whereby tissue elements are stained in definite sequence, and the staining solution is applied for specific periods of timeor until the desired intensity of coloring of the different tissue elements is attained.
Progressive staining
With this technique, the tissue is first overstained to obliterate the cellular details, and the excess stain is removed or decolorized front, unwanted parts of the tissue, until the desired intensity of color is obtained.
Regressive staining
is the selective removal of excess stain from the tissue during regressive staining in order that a specific substance may be stained distinctly from the surrounding tissues.
Differentiation (Decolorization)
uses more than one chemical stain to better differentiate between various microorganisms or structures/cellular components of a single organism
Differential staining
technique entails the use of specific dyes which differentiate particular substances by staining them with a color that is different from that of the stain itself (metachromasia).
Metachromatic staining
is a process where specific tissue elements aredemonstrated, not by stains, but by colorless solutions of metallic salts which arether by reduced by the tissue, producing an opaque, usually black deposit on thesurface of the tissue or bacteria.
Metallic impregnation
is the selective staining of living cell constituents, demonstrating cytoplasmic structures by phagocytosis of the dye particle cytoplasmic phagocytosis), or by staining of pre-existing cellular components
Vital staining
done by injecting the dye into any part ofthe animal body either intravenous, intraperitoneal or subcutaneous), producing specific coloration of certain cells, particularly those of the reticulo-endothelialsystem.
Intravital staining
a method of staining used in microscopy to examine living cells that have been removed from an organism
Supravital staining
is the corner stone of tissue-based diagnosis. The process stains thin tissue sections so that pathologists can visualize tissue morphology. The process uses a hematoxylin dye to stain cell nuclei (and other parts) blue and an eosin dye to stain other structures pink or red
Hematoxylin and Eosin (H&E) staining
are substances that combine with the tissue and the staining solution, forming a “bridge” that allows staining reaction tо takе place
Mordants