Staining Flashcards
What is the Direct staining method?
A labeled Ab of known specificity is used to identify Ag in patient tissue.
What is the Indirect staining method?
The patient’s serum is added to tissue sections containing known Ag to test the patient for the presence of Ab to those Ag. The patient’s serum can also be added to bacteria.
What is the Unlabeled/ Soluble Enzyme Immune Complex method?
A 3 step method involving a primary Ab, secondary Ab and a soluble enzyme-antienzyme complex.
What is the Avidin-biotin complex (ABC) method?
The primary Ab is followed by a secondary biotinylated Ab which bond irreversibly to Avidin and forms a complex.
What are three main benefits of the ABC method?
- There is low background staining
- Sensitivity can be up to 40X other immunoperoxidase methods
- Ab may be used at higher dilutions than in other methods.
What is polymeric detection?
The “gold standard” of IHC staining in which either a single strand (monomeric) or multiple strand (polymeric) molecules are fused with the secondary Ab which eliminates the need for ABC or patient serum. We did this in Vancouver.
Which detection system works better: monomeric or polymeric?
Monomeric, because there is increased sensitivity and Ab binding site penetration with single strand molecules. Turnaround time is also decreased.
What is the general procedure for polymeric detection?
Ab-polymer-chromogen.
What neoplasm does BCL2 test for?
Follicular lymphoma.
What neoplasm does CD117 test for?
Gastrointestinal stromal tumor.
What neoplasm does CD15 test for?
Hodgkin’s lymphoma.
What neoplasm does CD20 test for?
B cell lymphoma.
What neoplasm does CD3 test for?
T cell lymphoma.
What neoplasm does CK20 test for?
Colon carcinoma.
What neoplasm does Cytokeratin (Pankeratin) test for?
Carcinoma in general.
What neoplasm does Desmin test for?
Leiomyoma (smooth muscle tumor, common in uterus).
What neoplasm does ER test for?
Breast carcinoma.
What neoplasm does Glial Fibrillary Acidic Protein (GFAP) test for?
Glioblastoma multiforme.
What neoplasm does Gross Cystic Disease Fluid Protein (GCDFP-15) test for?
Breast ductal carcinoma.
What neoplasm does HER2 test for?
Breast carcinoma.
What neoplasm does HMB-45 test for?
Melanoma.
What neoplasm does CD45 test for?
Lymphoma.
What is another term for CD45?
Leukocyte common Ag.
What neoplasm does Melan-A test for?
Melanoma.