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HCT > staining > Flashcards

staining Flashcards

1
Q

dye component that confers color by altering the light resonance properties of a compound

A

chromophore

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2
Q

refers to a benzene containing chromophoric groups

A

chromogen

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3
Q

group responsible for tissues to bind firmly to a dye

A

auxochrome

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4
Q

collective term for synthetic/artificial (coal tar) dyes

A

aniline dyes

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5
Q

combination of immunologic & histochemical techniques that allow phenotypic markers to be detected

A

immunohistochemical staining

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6
Q

staining whereby various constituents of tissues are studied through chemical reactions that will permit microscopic localization

A

histochemical staining

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7
Q

staining whereby tissue components are demonstrated in sections by direct interaction with a dye

A

histologic staining

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8
Q

refers to selective staining of living cell constituents

A

vital staining

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9
Q

recommended stain for mitochondria

A

Janus Green

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10
Q

recommended stain for the reticuloendothelial system

A

Tryphan Blue

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11
Q

vital staining by means of injecting the dye into any part of the animal

A

intravital staining

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12
Q

vital staining by means of staining living cells immediately after removal from the living body

A

supravital staining

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13
Q

best vital stain

A

neutral red

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14
Q

selective stain for neutral fats

A

oil red O

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15
Q

selective stain for hemosiderin

A

Perl’s Prussian Blue

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16
Q

selective stain for polysaccharides

A

periodic acid schiff

17
Q

selective stain for elastic fibers

A

Weigert’s Elastic stain

18
Q

staining process that stains various tissue elements with little differentiation except between the nucleus and cytoplasm

A

routine staining

19
Q

staining is continued until the desired intensity of the color of the different tissue element is attained

A

progressive staining

20
Q

tissues are overstained and the excess dye is removed selectively until the desired intensity is obtained

A

regressive staining

21
Q

selective removal of excess stain to stain specific structures differentially from the rest of the surrounding tissue

A

differentiation/decolorization

22
Q

differentiator that acts by combining with the metal, breaking the latter’s union with the tissue or cell

A

acid differentiators

23
Q

differentiator that acts by oxidizing the dye to a colorless ubstance

A

oxidizing differentiator

24
Q

staining of tissues with a simple aqueous or alcoholic solution of the dye

A

direct staining

25
Q

staining of tissues with the addition of a mordant or accentuator to intensify the action of the dye

A

indirect staining

26
Q

substance that enhances the combination of the dye with the tissues

A

mordant

27
Q

substance that hastens the speed of intensity of the dye and has no chemical union between tissue and dye

A

accentuator

28
Q

substance that increases the rate of the staining action

A

accelerator

29
Q

process in which certain dyes stain certain tissues in a color that is different from that of the stain itself

A

metachromatic staining

30
Q

application of a different stain to provide contrast or background

A

counterstaining

31
Q

tree where hematoxylin is extracted from

A

Haematoxylum campechianum

32
Q

hematoxylin solution in which mordant and hematoxylin are mixed together

A

direct hematoxylin stain

33
Q

hematoxylin solution in which the mordant is not mixed together with the hematoxylin

A

mordant hematoxylin

34
Q

kind of eosin commonly used to compound Romanowsky stains

A

eosin B

35
Q

most common counterstain to alum hematoxylin in routine H&E

A

eosin Y

36
Q

effect of increasing dissolved salts (ionization) in the stain

A

decreased staining action

37
Q

effect of increased temperature to the stain

A

increased rate of penetration and staining action

38
Q

removal of excess paraffin so the stain can permeate the tissue sections

A

deparaffinization

HCT (5 decks)

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