staining Flashcards
dye component that confers color by altering the light resonance properties of a compound
chromophore
refers to a benzene containing chromophoric groups
chromogen
group responsible for tissues to bind firmly to a dye
auxochrome
collective term for synthetic/artificial (coal tar) dyes
aniline dyes
combination of immunologic & histochemical techniques that allow phenotypic markers to be detected
immunohistochemical staining
staining whereby various constituents of tissues are studied through chemical reactions that will permit microscopic localization
histochemical staining
staining whereby tissue components are demonstrated in sections by direct interaction with a dye
histologic staining
refers to selective staining of living cell constituents
vital staining
recommended stain for mitochondria
Janus Green
recommended stain for the reticuloendothelial system
Tryphan Blue
vital staining by means of injecting the dye into any part of the animal
intravital staining
vital staining by means of staining living cells immediately after removal from the living body
supravital staining
best vital stain
neutral red
selective stain for neutral fats
oil red O
selective stain for hemosiderin
Perl’s Prussian Blue
selective stain for polysaccharides
periodic acid schiff
selective stain for elastic fibers
Weigert’s Elastic stain
staining process that stains various tissue elements with little differentiation except between the nucleus and cytoplasm
routine staining
staining is continued until the desired intensity of the color of the different tissue element is attained
progressive staining
tissues are overstained and the excess dye is removed selectively until the desired intensity is obtained
regressive staining
selective removal of excess stain to stain specific structures differentially from the rest of the surrounding tissue
differentiation/decolorization
differentiator that acts by combining with the metal, breaking the latter’s union with the tissue or cell
acid differentiators
differentiator that acts by oxidizing the dye to a colorless ubstance
oxidizing differentiator
staining of tissues with a simple aqueous or alcoholic solution of the dye
direct staining
staining of tissues with the addition of a mordant or accentuator to intensify the action of the dye
indirect staining
substance that enhances the combination of the dye with the tissues
mordant
substance that hastens the speed of intensity of the dye and has no chemical union between tissue and dye
accentuator
substance that increases the rate of the staining action
accelerator
process in which certain dyes stain certain tissues in a color that is different from that of the stain itself
metachromatic staining
application of a different stain to provide contrast or background
counterstaining
tree where hematoxylin is extracted from
Haematoxylum campechianum
hematoxylin solution in which mordant and hematoxylin are mixed together
direct hematoxylin stain
hematoxylin solution in which the mordant is not mixed together with the hematoxylin
mordant hematoxylin
kind of eosin commonly used to compound Romanowsky stains
eosin B
most common counterstain to alum hematoxylin in routine H&E
eosin Y
effect of increasing dissolved salts (ionization) in the stain
decreased staining action
effect of increased temperature to the stain
increased rate of penetration and staining action
removal of excess paraffin so the stain can permeate the tissue sections
deparaffinization
