sectioning

Question 1

simplest microtome invented by Paldwell Trefall in 1881

Answer 1

rocking microtome

Question 2

most common type of microtome that operates with a stage rotary action

Answer 2

rotary microtome

Question 3

microtome invented by Adams used for cutting celloidin-embedded sections

Answer 3

sliding microtome

Question 4

most dangerous type of microtome due to the movable exposed knife

Answer 4

sliding microtome

Question 5

sliding microtome used for cutting tough tissue blocks which may offer great resistance to the knife

Answer 5

base-sledge microtome

Question 6

microtome invented by Queckett that releases rapid intermittent bursts of carbon dioxide

Answer 6

freezing microtome

Question 7

ideal microtome used when histological demonstration of fat and certain neurological structures are to be studied

Answer 7

freezing microtome

Question 8

microtome commonly used for rapid prep. of urgent tissue biopsies for intraoperative diagnosis

Answer 8

cryostat

Question 9

microtome equipped with a gem grade diamond knife or broken plate glass for cutting sections for electron microscopy

Answer 9

ultrathin microtome

Question 10

one side of the knife is flat while the other is concave usually 25 mm in length

Answer 10

plane-concave knife

Question 11

both sides of the knife are concave usually 120 mm in length

Answer 11

biconcave knife

Question 12

both sides of the knife are straight usually 100 mm in length

Answer 12

plane-wedge knife

Question 13

knife recommended for frozen sections and extremely tough specimens

Answer 13

plane-wedge knife

Question 14

knife recommended for paraffin-embedded sections on a rotary microtome

Answer 14

biconcave knife

Question 15

the cold chamber in a cryostat is maintained between this temperature range

Answer 15

• 5 to - 30 C (ave. -20)

Question 16

this knife is brittle and expensive and is able to cut any type of resin block for electron microscopy

Answer 16

diamond knife

Question 17

angle formed between the cutting edges

Answer 17

bevel angle

Question 18

angle formed between the tissue block and the cutting edge of the knife between two smooth plane surfaces

Answer 18

clearance angle

Question 19

bevel angle is maintained at this rangle

Answer 19

27-32 degrees

Question 20

its purpose is for removing gross nicks and irregularities on the knife edge

Answer 20

honing (coarse sharpening)

Question 21

its purpose is to remove the burr and polish the cutting edge

Answer 21

stropping (fine sharpening)

Question 22

perfect and optimum cutting angle

Answer 22

15 degrees

Question 23

hone type that gives the best result

Answer 23

belgium yellow

Question 24

this hone is used only for badly nicked knives followed by either one of the first two knife sharpeners

Answer 24

fine carborondum