Stain - PPB

Question 1

when do you use PPB stain to stain a sample?

Answer 1

Lung tissue (pleural membrane)
bronchioalveolar lavage
sputum (a thick type of mucus made in your lungs)
skin

Question 2

what is the blue seen?

Answer 2

haemosiderin

Question 3

HCl

Answer 3

denatures the haemosiderin, releasing ferric iron

Question 4

Perl’s solution

Answer 4

contains potassium ferrocyanide which reacts w/ ferric iron to produce and insoluble BLUE compound

Question 5

what is the name of the blue compound formed after the reaction of Perl’s solution with ferric iron?

Answer 5

ferric ferrocyanide