spermatogenesis and sperm deposition Flashcards

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1
Q

what is spermatogenesis?

A

the production of spermatozoa ( the male gamete).

this occurs in the seminiferous tubules

starts in the basement membrane (seminiferous epithelium)

proliferate and mature towards the lumen

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2
Q

what are the 3 phases that spermatogenesis is split into?

A

spematocytogenesis (proliferation)

meiosis

spermiogenesis (differentiation)

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3
Q

how many spermatozoa can a dairy bull produce?

A

7.5 x 10000000000 spermatozoa a day

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4
Q

what is spermatocytogenesis?

A

the first phase of spermatogenesis

spermatogonia (stem cells) undergo mitotic division

3 different spermatogonia:
A - divides by mitosis roughly 2 times then becomes an intermediate
I (intermediate) then divide via mitosis to become B
B then divides to become a primary spermatocyte

number of divisions depends on the species
e.g. ram has 6 stages = 64 primary
where as a stallion has 8 = 256 primary
many degenerate

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5
Q

what occurs during the phase of meiosis?

A

the spermatocytes made in the first phase undergo meiosis, making secondary spermatids

secondary spermatids = genetically unique (crossing over in meiosis 1)

primary spermatocytes = longes lived germ cell in the process

secondary spermatocytes = short-lived (less than 2 days)

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6
Q

what occurs in the 3rd phase, spermiogenesis?

A

differentiation occurs - no division occurs
spheroid spermatid undergoes:
condensation of nucleus

formation of: acrosome, tail (flagellum) and a mitochondrial helix

produces spermatozoa with the potential to be motile and able to fertilise

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7
Q

how long does spermatogenesis take?

A

the length varies between species.

bull = 61 days
ram = 47 days
boar = 39 days
stallion = 55 days
dog = 62 days
tom = 47 days

the process occurs throughout seminiferous tubules - this gives a continuous release of spermatozoa

different areas at different stages

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8
Q

what is spermiation?

A

spermiation = the release of spermatozoa from sertoli cells

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9
Q

what are the characteristics of sperm?

A

surrounded by permeable plasma membrane

have a head and tail

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10
Q

what are the characteristics of the sperm head?

A

contains the nucleus (n)
compact chromatin
nuclear membrane surrounds the head

has an acrosome which is membrane bound
the acrosome contains hydrolytic enzymes

there is also a postnuclear cap/ postacrosomal region

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11
Q

what are the characteristics of a sperm tail?

A

there is a neckpiece that acts as the middle section containing lots of mitochondria as energy is required to drive tail

the tail has a principal piece and an end piece
throughout the tail, there is an axoneme:
containing central filaments and microtubules
there are also coarse fibres

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12
Q

where does the sperm travel?

A

starting in the seminiferous tubules

to the rete testis

to the epididymis

then through the vas daferens

onto the accessory glands - ampulla, vesicular glands, prostate, bulbourethral glands (cowpers)

finally onto the urethra

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13
Q

how is sperm deposited?

A

sperm have matured in the epididymis

non- ejaculated sperm is senescent and broken down

deposition occurs in 3 stages
1) erection
2) emission - super through vas daferens, mixes with seminal plasma and is now called semen
3) ejaculation - semen expelled from the penis

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14
Q

how does ejaculation occur?

A

contraction of muscles in the:

epididymis
vas daferens
accessory glands
urethra

it is driven by testosterone

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15
Q

how is the erection caused?

A

it is activated by the central nervous system

causes increased blood flow to the erectile tissue

in the stallion/ tom/ dog = increase in diameter of penis

in the bull/ ram/ boar = small increase in diameter and relaxation of retractor muscle = sigmoid flexure straightens

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16
Q

what is semen?

A

it is the sperm + seminal plasma
sperm is immotile without seminal plasma

there are 3 fractions:
pre sperm - to lubricate and clean the urethra

sperm rich - 80%-90% of sperm, there is a biomechanical component

post sperm - gel fraction in boar and stallion

17
Q

what does seminal plasma provide?

A

transport fluid

nutrition
energy - glucose in stallion
fructose in bull/ram/boar/dog

protection:
antioxidant = ergothioneine
osmotic pressure - citric acid
pH