spectroscopy

Question 1

what are the components of a spectrophotometer

Answer 1

1.light source
2.monochromator
3.sample holder
4.detector
5.display/read out

Question 2

what does the light source do?

Answer 2

light source provides UV and visible light to the sample

Question 3

what does the monochromator do

Answer 3

splits the light into specific wavelengths and selects the desired wavelength

Question 4

what is the sample holder used for

Answer 4

contains the solution that will be analysed inside a plastic cuvette

Question 5

what does the detector do?

Answer 5

the detector measures the intensity of light transmitted through the sample

Question 6

display/readout

Answer 6

shows the absorbance or transmittance

Question 7

what is the operating principle of a spectrophotometer

Answer 7

-light passes through the sample and certain wavelengths are absorbed depending on electronic transitions.
-the detector measures the remaining transmitted light, and absorbance is calculated using beer-lamber law.

Question 8

state beer lambert law

Answer 8

the beer lambert law states that the absorbance (A) of light by a solution is directly proportional the concentration of the absorbing species, the path length of light through the solution and molar absorptivity.

Question 9

2 limitations of beer lambert law

Answer 9

1. the beer lambert law does not work at very high concentrations of the absorbing substance.as it would deviate the absorptivity coefficients
   2.instument limitations ;waffle talk about how it could cause reading inaccuracies and deviate from the law