Spectrophotometer Flashcards
What is Beer’s law?
- Absorbance is proportional to concentration
- Linear
What happens when beer’s law cannot be applied?
A dilution has to be done
What is one point calibration?
Calculation of comparison of the known standard, concentration and it’s corresponding absorbance to the absorbance of the unknown value.
What is the equation for Concentration calculations.
Concentration of standard over absorbance of standard is equal to concentration of unknown over absorbance of unknown.
How are unknown patient samples read?
They are compared on a standard curve within a known region (linear).
What are the parts on a spectrophotometer?
Light
Monochromator
cuvette
detector
What comprises the electronic measuring device?
Photoelectric cell and the galvanometer
How does the electronic measuring device work?
The galvanometer records the amount of current it receives from the photoelectric cell.
What is run in parallel to samples in order to remove error from colour reagents
A blank
What is stray light causes by?
Scratches and Dust
Finger prints
oil residue
What is stray light
wavelength outside of the band transmitted by monochromator
What is spec measured in?
Transmittance and or absorbance
What is the difference between transmittance and absorbance?
T = logarithmic
A = linear
What is the equation for absorbance?
Absorbance = 2 - log %T
What is an example of wet chemistry?
Absorbance spec
