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Clinical Bacteriology > Specimen Management > Flashcards

Specimen Management Flashcards

1
Q

WHEN should specimen be collected?
DURING ______________
WITHIN _______________
BEFORE ______________

A

During Acute Phase
Within 2-3 Days for Viral Infection
Before Antibiotics are Administered

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2
Q

Container for Abscess, Lesions, Wounds

A

Swabs (aerobic) or sterile tube (for aspirates)

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3
Q

For Abscess, Lesions, Wounds:

Wipe around the area with _______ or _______

A

sterile saline or 70% alcohol

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4
Q

Container for Urine

A

Clean, screw cap

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5
Q

Container for Sputum

A

Clean, screw cap

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6
Q

Sputum collection procedure for deep cough only

A

Expectorated Sputum

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7
Q

Sputum collection procedure with inhale solution

A

Induced Sputum

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8
Q

Container for Stool

A

Clean, screw cap

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9
Q

Bacterial stool are preferably performed _________

A

once a day for 3 days

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10
Q

Container for Blood

A

Blood culture media set

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11
Q

For Blood Collection, disinfect site with _______ and _______

A

70% alcohol and Povidone iodine

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12
Q

Container for CSF

A

Clean, screw cap

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13
Q

Which CSF tube is used for bacteriology laboratory use?

A

Tube 2

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14
Q

Specimen label must include

A

Patient’s name
Hospital number/DOB
Date and TOC
Source

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15
Q

Ideally, collected specimen must be transported to the laboratory within ________ and not longer than ________

A

30 minutes,

2 hours

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16
Q

CSF - transport within _______ after

collection

A

15 mins

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17
Q

Anaerobic specimen - transport not longer than

_________.

A

10 mins

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18
Q

Sensitive to change in temperature

A

Neisseria meningitidis

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19
Q

Sensitive to change in PH

A

Shigella

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20
Q

Transport condition of Body fluids

A

Immediately at RT

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21
Q

Transport condition of Corneal scrapings

A

Immediately at RT

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22
Q

Transport condition of bone

A

Immediately at RT

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23
Q

Transport condition of gastric aspirate

A

Immediately at RT

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24
Q

Transport condition of prostatic samples

A

Immediately at RT

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25
Q

Transport condition of suprapubic aspirate

A

Immediately at RT

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26
Q

Transport condition of tissue

A

Immediately at RT

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27
Q

Transport condition of gastric biopsy

A

Within 1 hour at RT

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28
Q

Transport condition of stool

A

Within 1 hour at RT

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29
Q

Transport condition of abscess, lesions, wound

A

Within 2 hours at RT

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30
Q

Transport condition of blood

A

Within 2 hours at RT

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31
Q

Transport condition of BMA

A

Within 2 hours at RT

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32
Q

Transport condition of genital tract specimens

A

Within 2 hours at RT

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33
Q

Transport condition of respiratory tract specimens

A

Within 2 hours at RT

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34
Q

Transport condition of urine

A

Within 2 hours at RT

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35
Q

Transport condition of urine samples from foley catheter

A

Within 2 hours at 4°C

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36
Q

Transport condition of Hair, nails, skin scrapings (Fungal

culture)

A

Within 24 hours at RT

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37
Q

A) Transport condition of specimens with transport medium/preservatives

B) What are these specimens?

A

A) Within 24 hours at RT

B) Stool, Rectal Swab, Urine

38
Q

Boric acid is used for _______ to maintain quality and quantity of bacteria

A

Urine

39
Q

Cary-blair media is used for

A

Stool

40
Q

Anticoagulants for specimen preservation

A

Sodium Polyanethol Sulfate, Heparin

41
Q

Anticoagulant that inhibits growth of gram(+) bacteria and yeast for viral cultures

A

Heparin

42
Q

transport medium used for Neisseria gonorrhea suspected specimen

A

JEMBEC

43
Q

Storage Condition for CSF

A

37°C

44
Q

Storage Condition for Urine

A

4°C

45
Q

Storage Condition for Stool

A

4°C

46
Q

Storage Condition for Viral specimen

A

4°C

47
Q

Storage Condition for Swabs

A

4°C

48
Q

Storage Condition for Foreign Devices

A

4°C

49
Q

Storage Condition for Serum (for 1 week)

A

-20°C

50
Q

Storage Condition for tissues

A

-70°C

51
Q

Storage Condition for specimens for long term storage

A

-70°C

52
Q

Expectorated sputum with ________ and ________ in Gram staining are rejected

A

<25 WBCs and >10 epithelial cells

53
Q 
Descriptions of the Levels of Specimen Prioritization
1 -
2 - 
3 - 
4 -
A

1 - Critical/invasive
2 - Unpreserved
3 - Quantitation Required
4 - Preserved

54
Q

Level of Specimen Prioritization of Amniotic Fluid

A

1 - Critical/invasive

55
Q

Level of Specimen Prioritization of Blood

A

1 - Critical/invasive

56
Q

Level of Specimen Prioritization of Brain

A

1 - Critical/invasive

57
Q

Level of Specimen Prioritization of CSF

A

1 - Critical/invasive

58
Q

Level of Specimen Prioritization of Heart Valves

A

1 - Critical/invasive

59
Q

Level of Specimen Prioritization of Pericardial Fluid

A

1 - Critical/invasive

60
Q

Level of Specimen Prioritization of Body Fluids

A

2 - Unpreserved

61
Q

Level of Specimen Prioritization of Bone

A

2 - Unpreserved

62
Q

Level of Specimen Prioritization of Drainage from Wounds

A

2 - Unpreserved

63
Q

Level of Specimen Prioritization of Stool

A

2 - Unpreserved

64
Q

Level of Specimen Prioritization of Sputum

A

2 - Unpreserved

65
Q

Level of Specimen Prioritization of Tissue

A

2 - Unpreserved

66
Q

Level of Specimen Prioritization of Catheter Tip

A

3 - Quantitation Required

67
Q

Level of Specimen Prioritization of Urine

A

3 - Quantitation Required

68
Q

Level of Specimen Prioritization of Tissue for quantitation

A

3 - Quantitation Required

69
Q

Level of Specimen Prioritization of stool in preservative

A

4 - Preserved

70
Q

Level of Specimen Prioritization of urine in preservative

A

4 - Preserved

71
Q

Level of Specimen Prioritization of swabs in holding medium (aerobic and anaerobic)

A

4 - Preserved

72
Q

Most cultures grow between __°C and __°C in incubation

A

35°C and 37°C

73
Q

Time required in incubation

A

most are held for 2-3 days

some are held for 5-7 days

74
Q

-
-

A

Sodium Hypochlorite (10%)
Chlorine Dioxide
Peracetic Acid

75
Q

-

A

Moist Heat

Dry Heat

76
Q

process of destruction of all forms of microbial life

A

STERILIZATION

77
Q

lowest temperature at which all organisms in particular liquid suspension will be killed in 10 minutes

A

THERMAL DEATH POINT

78
Q

minimal length of time for all bacteria in particular liquid culture to be killed at a given temperature.

A

THERMAL DEATH TIME

79
Q

time in which 90% of bacterial population will be killed at a given temperature

A

DECIMAL REDUCTION TIME

80
Q

kills microorganisms by oxidation effects

A

Dry Heat

81
Q

mode of action: burning contaminants into ashes

A

direct flaming

82
Q

used for inoculating loops/needles

A

direct flaming

83
Q

most common method of treating infectious waste

A

incineration

84
Q

burning materials to ashes at temperatures 890°C-980°C

A

incineration

85
Q

kills microorganisms by coagulation of proteins (denaturation)

A

MOIST HEAT

86
Q

kills vegetative forms of bacterial pathogen

A

BOILING

87
Q

mode of action: protein denaturation within 15 mins

A

BOILING

88
Q

Effective for viruses and fungi, (less/more?) effective on endospores

A

BOILING, less

89
Q

use of temperature up to 100°C at normal atmospheric pressure

A

FLOWING STEAM

90
Q

mode of action of flowing steam

A

protein denaturation

91
Q

Autoclaving - use of steam at

A

15 psi, temp of 121°C for 15 minutes

92
Q

mode of action - irreversible denaturation of engines and structural proteins

A

Autoclaving

Clinical Bacteriology (5 decks)

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