Laboratory Safety

Question 1

Works by denaturing nucleic acids and enzymes in the bacteria

Answer 1

MOIST HEAT

Question 2

Principle: Steam under pressure

Answer 2

Autoclaving

Question 3

Autoclaving

Temperature:
Time:
Pressure:

Answer 3

Temperature: 121°C
Time: 15 mins
Pressure: 15 lbs/psi

Question 4

Items such as media, liquids, and instruments are usually autoclaved for

Temperature:
Time:

Answer 4

Temperature: 121°C
Time: 15 mins

Question 5

Infectious waste are autoclaved for

Temperature:
Time:

Answer 5

Temperature: 132°C
Time: 30-60 mins

Question 6

in pre-vacuum sterilizer, autoclave __°C for ______ (time)

Answer 6

134°C for 18 mins

Question 7

in gravity displacement sterilizer, autoclave __°C for ______ (time)

Answer 7

132°C for 1 hour

Question 8

Hot-air sterilization (oven)

Temperature:
Time:

Answer 8

Temperature: 160°C - 180°C
Time: 1.5-3 hours

Question 9

Incineration

Temperature:

Answer 9

870°C-980°C

Question 10

the method of choice for antibiotic solutions, toxic chemicals, radioisotopes, vaccines, and carbohydrates, which are all heat-sensitive

Answer 10

FILTRATION

Question 11

Non-Ionizing or Ionizing?

Less energy

Answer 11

Non-Ionizing

Question 12

Non-Ionizing or Ionizing?

Forms from high-energy radiation

Answer 12

Ionizing

Question 13

Non-Ionizing or Ionizing?

Uses UV light

Answer 13

Non-Ionizing

Question 14

Non-Ionizing or Ionizing?

Causes thymine dimers to form between adjacent thymines within a single strand of DNA

Answer 14

Non-Ionizing

Question 15

Non-Ionizing or Ionizing?

Strong enough to pass through the cells

Answer 15

Ionizing

Question 16

Non-Ionizing or Ionizing?

Includes X-rays, gamma rays, and high energy electron beams

Answer 16

Ionizing

Question 17

Non-Ionizing or Ionizing?

Used to sterilize materials that cannot be autoclaved such as plastic petri dishes,
gloves, intravenous tubing, and other latex and plastic items use for patient care

Answer 17

Ionizing

Question 18

Other name for Regular/Classic

Answer 18

Holding or Batch

Method

Question 19

Regular/Classic

Temperature:
Time:

Answer 19

Temperature: 63°C
Time: 30 mins

Question 20

High Temperature Short Time

Temperature:
Time:

Answer 20

Temperature: 72°C
Time: 15 secs

Question 21

Method used to reduce number of bacteria while

preserving the quality of milk

Answer 21

High Temperature Short Time

Question 22

Method used to reduce the number of bacteria

Answer 22

Regular/Classic

Question 23

Ultra High Temperature

Temperature:
Time:

Answer 23

Temperature: 140°C
Time: 3 secs

Question 24

___________ pasteurized milk can be stored for
long time in sealed containers without being
refrigerated

Answer 24

Ultra High Temperature

Question 25

Temp of Refrigeration:

Temp of Deep-Freezing:

Answer 25

Temp of Refrigeration: 2°C-8°C

Temp of Deep-Freezing: -2°C

Question 26

Type of pressure that kills bacteria, yeasts, molds, and

parasites.

Answer 26

high-pressure processing

Question 27

Type of pressure that inhibits bacterial cell growth as the water leaves

Answer 27

high – salt/sugar concentration

Question 28

Used for gaseous sterilization and is a form of cold sterilization

Answer 28

Ethylene oxide (EtO)

Question 29

• Is commonly used in
solution at a
concentration of 37%
(formalin)

• Widely used as chemical
fixative (used for tissue
specimens and
embalming fluid)

Answer 29

Formaldehyde vapor and
vapor-phase hydrogen
peroxide

Question 30

• Has two reactive aldehyde groups, allowing it to act more quickly than formaldehyde
• Used as 2% solution

Answer 30

Glutaraldehyde

Question 31

most often used in the
form of sodium hypochlorite
(NaOCl), known as
household bleach

Answer 31

Chlorine

Question 32

dilution of bleach

Answer 32

1:10

Question 33

Is prepared either as tincture with alcohol, or as an Iodophor coupled to a neutral polymer, such as povidone-iodine

Answer 33

Iodine

Question 34

instilled in the eyes of
newborn to prevent
infections with Neisseria
gonorhoeae

Answer 34

1% silver nitrate

Question 35

used to disinfect benchtops or other surfaces in the

laboratory

Answer 35

Quaternary ammonium compounds

Question 36

Found in antibacterial soaps, textile

Answer 36

Phenolic compound

triclosan

Question 37

Commonly used peroxygen is _________; skin antiseptic

Answer 37

hydrogen peroxide

Question 38

Can be used as liquid or plasma sterilant;

- More effective than H2O2

Answer 38

Peracetic acid