Specimen Collection, Transport Media,

Question 1

Appropriate time of collection – _ (early disease process) within _ hours of disease

Answer 1

Acute phase reaction; 72

Question 2

ways of specimen collection

Answer 2

1. aspirated secretion
2. swabs (synthetic swabs)

Question 3

synthetic seabs

Answer 3

Dacron or Nylon

Question 4

Consist of buffered isotonic solution + some type of protein + antibiotic

Answer 4

transport media

Question 5

transport media is consist of

Answer 5

buffered isotonic solution + some type of protein + antibiotic

Question 6

example of transport media

Answer 6

SALEHE

Stuarts,
Amies,
Leibovitz Emory,
Hunk’s Balance Salt Solution (HBSS)
Eagle Tissue Culture

Question 7

Most widely used for virus isolation (shell vial)

Answer 7

cell cultures

Question 8

type of cell cultures

Answer 8

1. primary cell culture
2. semi-continuous cc
3. continuous cc

Question 9

derived from animal tissues; very expensive; and example

Answer 9

primary cc
- monkey kidney

Question 10

monkey kidney is excellent for

Answer 10

myxovirus (RNA)

Question 11

derived from embryonic tissues; and example

Answer 11

semi-continuous;
- Human Embryonic kidney
- skin fibroblasts

Question 12

derived from tumor cells / malignant tissue; and example

Answer 12

continuous cc
- HeLa, Vero, Hep2, LLC-MK2, MDCK