SPECIMEN COLLECTION & HANDLING Flashcards
How long should sexual abstinence last for optimal sperm motility and volume?
- It should be at least 2 days but not more than 7 days
What is the impact of prolonged sexual abstinence on sperm?
- Prolonged sexual abstinence can result in higher sperm volume but decreased motility.
How many sperm samples should be collected for fertility testing according to WHO guidelines?
- WHO recommends collecting 2 or 3 sperm samples
- With a minimum interval of Not less than 7 days and not 3 weeks between collections.
2 abnormal sperm samples considered ——- in fertility testing?
significant.
What are some reasons for abnormal sperm samples in fertility testing according to WHO?
-sperm morphology (shape)
-the quantity of sperm.
Where should the specimen ideally be collected?
- The specimen should ideally be collected in the room provided by the laboratory.
What should be done if the specimen cannot be collected in the laboratory room?
- If not collected in the laboratory room
- The specimen should be kept at room temperature and delivered to the laboratory within 1 hour of collection
- To avoid decreasing motility of sperm cells.
How should specimens be stored while awaiting testing?
- Specimens awaiting testing should be kept in an incubator at 37°C.
What is the recommended method of specimen collection?
- The specimen should be collected by masturbation.
What type of condoms should be used for specimen collection?
- Only non-lubricant-containing rubber or polyurethane condoms should be used.
Why are ordinary condoms not acceptable for specimen collection?
- Ordinary condoms are not acceptable
- Because they contain spermicides, which can affect the specimen.
What is the purpose of specifying the specimen volume?
- Specifying the specimen volume is necessary for the computation.
What is the normal volume range for semen in a semen analysis?
- 2 - 5 mL.
How is viscosity assessed in a semen analysis, and what indicates abnormal viscosity?
- Observing how semen pours
- If it forms stringy droplets longer than 2 cm, it is considered abnormal viscosity.
What is the ideal pH range for semen in a semen analysis, and why is it slightly alkaline?
- 7.2 - 8.0
- It is slightly alkaline due to the fluid produced by the bulbourethral gland.
What is the minimum sperm concentration considered normal in a semen analysis?
- Greater than 20 million/mL.
What is the minimum sperm count considered normal in a complete ejaculate during a semen analysis?
- Greater than 40 million.
What is the minimum motility percentage within 1 hour considered normal in a semen analysis?
- Greater than 50%.
What is the minimum quality value or criteria considered normal in a semen analysis?
- Greater than 2.0 or meeting the criteria a, b, c.
What are the reference values for normal sperm morphology according to strict criteria and routine criteria?
- Normal sperm morphology are >14% using strict criteria
- > 30% using routine criteria.
What is the recommended maximum count of round cells in semen analysis, and why is it important to differentiate between white blood cells (WBC) and spermatid cells?
- <1.0 million/mL
- Differentiating between the two helps in accurate diagnosis and treatment.
What does a sperm motility grade of 4.0 indicate according to WHO standards?
- A sperm motility grade of 4.0 indicates rapid, straight-line motility.
- WHO A
Which grade represents slower speed and some lateral movement in sperm motility grading?
- Grade 3.0 represents slower speed and some lateral movement in sperm motility.
- WHO B
What is the motility action associated with a sperm motility grade of 2.0 B according to WHO?
slow forward progression with noticeable lateral movement.
