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AUBF Seminal Fluid (Seminalysis) > SPECIMEN COLLECTION & HANDLING > Flashcards

SPECIMEN COLLECTION & HANDLING Flashcards

1
Q

How long should sexual abstinence last for optimal sperm motility and volume?

A
  • It should be at least 2 days but not more than 7 days
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2
Q

What is the impact of prolonged sexual abstinence on sperm?

A
  • Prolonged sexual abstinence can result in higher sperm volume but decreased motility.
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3
Q

How many sperm samples should be collected for fertility testing according to WHO guidelines?

A
  • WHO recommends collecting 2 or 3 sperm samples
  • With a minimum interval of Not less than 7 days and not 3 weeks between collections.
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4
Q

2 abnormal sperm samples considered ——- in fertility testing?

A

significant.

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5
Q

What are some reasons for abnormal sperm samples in fertility testing according to WHO?

A

-sperm morphology (shape)
-the quantity of sperm.

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6
Q

Where should the specimen ideally be collected?

A
  • The specimen should ideally be collected in the room provided by the laboratory.
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7
Q

What should be done if the specimen cannot be collected in the laboratory room?

A
  • If not collected in the laboratory room
  • The specimen should be kept at room temperature and delivered to the laboratory within 1 hour of collection
  • To avoid decreasing motility of sperm cells.
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8
Q

How should specimens be stored while awaiting testing?

A
  • Specimens awaiting testing should be kept in an incubator at 37°C.
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9
Q

What is the recommended method of specimen collection?

A
  • The specimen should be collected by masturbation.
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10
Q

What type of condoms should be used for specimen collection?

A
  • Only non-lubricant-containing rubber or polyurethane condoms should be used.
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11
Q

Why are ordinary condoms not acceptable for specimen collection?

A
  • Ordinary condoms are not acceptable
  • Because they contain spermicides, which can affect the specimen.
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12
Q

What is the purpose of specifying the specimen volume?

A
  • Specifying the specimen volume is necessary for the computation.
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13
Q

What is the normal volume range for semen in a semen analysis?

A
  • 2 - 5 mL.
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14
Q

How is viscosity assessed in a semen analysis, and what indicates abnormal viscosity?

A
  • Observing how semen pours
  • If it forms stringy droplets longer than 2 cm, it is considered abnormal viscosity.
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15
Q

What is the ideal pH range for semen in a semen analysis, and why is it slightly alkaline?

A
  • 7.2 - 8.0
  • It is slightly alkaline due to the fluid produced by the bulbourethral gland.
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16
Q

What is the minimum sperm concentration considered normal in a semen analysis?

A
  • Greater than 20 million/mL.
17
Q

What is the minimum sperm count considered normal in a complete ejaculate during a semen analysis?

A
  • Greater than 40 million.
18
Q

What is the minimum motility percentage within 1 hour considered normal in a semen analysis?

A
  • Greater than 50%.
19
Q

What is the minimum quality value or criteria considered normal in a semen analysis?

A
  • Greater than 2.0 or meeting the criteria a, b, c.
20
Q

What are the reference values for normal sperm morphology according to strict criteria and routine criteria?

A
  • Normal sperm morphology are >14% using strict criteria
  • > 30% using routine criteria.
21
Q

What is the recommended maximum count of round cells in semen analysis, and why is it important to differentiate between white blood cells (WBC) and spermatid cells?

A
  • <1.0 million/mL
  • Differentiating between the two helps in accurate diagnosis and treatment.
22
Q

What does a sperm motility grade of 4.0 indicate according to WHO standards?

A
  • A sperm motility grade of 4.0 indicates rapid, straight-line motility.
  • WHO A
23
Q

Which grade represents slower speed and some lateral movement in sperm motility grading?

A
  • Grade 3.0 represents slower speed and some lateral movement in sperm motility.
  • WHO B
24
Q

What is the motility action associated with a sperm motility grade of 2.0 B according to WHO?

A

slow forward progression with noticeable lateral movement.

25
Q

What does a sperm motility grade of 1.0 signify in terms of motility action?

A
  • A sperm motility grade of 1.0 signifies no forward progression.
  • WHO C
26
Q

How is a sperm sample graded when it shows no movement according to WHO standards?

A
  • When there is no movement in a sperm sample
  • It is graded as 0, indicating no movement (paikot ikot lang, hindi nag momove forward).
  • WHO D
27
Q

What is Progressive Motility (PM) in alternative sperm motility grading criteria?

A
  • Progressive Motility (PM) refers to sperm that are moving linearly or in a large circle.
28
Q

How is Non-Progressive (NP) sperm defined in this grading criteria?

A
  • Non-Progressive (NP) sperm are those that are moving but lack any progression.
29
Q

What does Immotility (IM) indicate in the context of alternative sperm motility grading criteria?

A
  • Immotility (IM) means that there is no movement observed in the sperm.

AUBF Seminal Fluid (Seminalysis) (5 decks)

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