specimen collection and processing of hemostasis testing Flashcards
what are the two methods in hemostasis testing
a. butterfly method
b. ETS
c. one syringe method
d. two syringe method
Methods of collection
> Two syringe method
> Evacuated tube technique
These two methods are preferred because it requires that a FEW VOLUME OF BLOOD SHOULD BE DISCARDED FIRST then the next volume of blood is collected, this is done TO PREVENT POSSIBLE CONTAMINATION OF TISSUE FLUID that contains thromboplastin which somehow alters results obtain from clotting tesT
What is the ratio of blood and anticoagulant in hemostasis
a. 1:9
b. 1:1
c. 9:1
d. 4:1
Anticoagulant
- 0.105 to 0.109M (3.2%) buffered Sodium citrate
Ratio of Blood to anticoagulant – (9:1)
what is the anticoagulant used in glass beaded retention test
a. EDTA
b. sodium citrate
c. heparin
d. sodium fluoride
CITRATE is the anticoagulant of choice for PLATELET FUNCTION TESTING
except for the GLASS BEADED RETENTION TEST, which require HEPARIN.
what are the labile factors
a. V
b. VII
c.IV
d, VIII
Labile factors (V and VIII) deteriorate at RT and increased pH
Labile factors (V and VIII) deteriorate at_____ and increased pH
a. cold temperature
b. room temperature
c. body temperature
Labile factors (V and VIII) deteriorate at RT and increased pH
Factors VII and XI prematurely activates when place at a cold temperature (4’C)
(sa 711 MALAMIG)
Factors ___________ prematurely activates when place at a cold temperature (4’C)
a. V
b. VII
c. X
d. XI
Labile factors (V and VIII) deteriorate at RT and increased pH
Factors VII and XI prematurely activates when place at a cold temperature (4’C)
Aspirin and other NSAID’s inhibit cyclooxygenase pathway
o Platelet function test: __________ abstinence from the following drugs
a. 24 hours
b. 2 weeks
c. 1 week
d. 3 days
Aspirin and other NSAID’s inhibit cyclooxygenase pathway
o Platelet function test: 1week abstinence from the following drugs
o Bleeding time test: 24 hours aspirin intake is prohibited
Bleeding time test: ______ aspirin intake is prohibited
a. 24 hours
b. 2 weeks
c. 1 week
d. 3 days
Aspirin and other NSAID’s inhibit cyclooxygenase pathway
o Platelet function test: 1week abstinence from the following drugs
o Bleeding time test: 24 hours aspirin intake is prohibited
Specimen processing for APTT is valid for_____
a. 24 hours
b. 2 weeks
c. 1 week
d. 4 hours
Coagulation specimen should be centrifuged within 1 hour of obtaining the sample
Specimen processing for PT is valid within 24 hours
Specimen processing for APTT is valid for 4 hours (APaTT)
The ___, ___, ___ are prolonged when Fibrinogen level is ≤100mg/dl
a. platelet
b. PT
c. TcT
d. PTT
The PT and APTT, TCT are prolonged when Fibrinogen level is ≤100mg/dl
the PRP contains how many platelets
a. <10,000/uL
b. 200,000/ uL
c, 300, 000/ uL
d. >100,00/ul
PRP (platelet RICH plasma)
> Can be obtained by centrifuging WB collected from 3.2% Sodium Citrate at 50g x for 30 minutes
> It contains 200,000 to 300,000 /ul platelets (200 to 300 x109/L platelets)
> It is used for platelet aggregometry or platelet function test
which of the ff is used for platelet aggregometry
a. PRP
b. PPP
PRP (platelet RICH plasma)
> Can be obtained by centrifuging WB collected from 3.2% Sodium Citrate at 50g x for 30 minutes
> It contains 200,000 to 300,000 /ul platelets (200 to 300 x109/L platelets)
> It is used for platelet aggregometry or platelet function test
the PPP contains how many platelets
a. <10,000/uL
b. 200,000/ uL
c, 300, 000/ uL
d. >100,00/ul
PPP (Platelet Poor Plasma)
> Can be obtained by centrifuging WB collected from 3.2% Sodium Citrate at 1500g x for 15 minutes
> It contains less than 10,000/ul platelets
> It is used for clot-based coagulation test (E.g PT and APTT)
