lab test for primary hemostasis ii

Question 1

Normal clot retraction requires a normal
number of functioning______ , _____ __________

a. platelets
b. calcium
c. ATP
d. Fibrinogen

Answer 1

NORMAL CLOR RETRACTION requires a normal
number of functioning
    PLATELETS
    CALCIUM
     ATP
     FIBRINOGEN

ANSWERl AOTA

Question 2

____________ is responsible for clot retraction

A. Epinephrine

b. ADP
c. Actomyosin
d. Thrombostenin

Answer 2

ACTOMYOSIN OR THROMBOSTENIN is responsible for clot retraction

Question 3

reflects the number and quality of platelets, fibrinogen concentration, fibrinolytic activity, and packed red
cell volume

A. platelet adhesiveness
B. clot retraction tine
C. capillary Fragility test
d. AOTA

Answer 3

CLOT RETRACTION TIME

❖ The contractile abilities of platelets also result in the contraction of formed clots. Normal clot retraction requires a normal number of functioning platelets, calcium, ATP and fibrinogen

❖ Actomyosin or thrombostenin is responsible for clot retraction

❖ Clot retraction REFLECTS THE NUMBER AND QUALITY of platelets, fibrinogen concentration, fibrinolytic activity, and packed red cell volume (Turgeon)

Question 4

which of the ff clot retraction time method has a result formation of Dumpling/ droplet

a. Castor oil
b. hirschboek
c. stefanini
d. macfarlane

Answer 4

a. CASTOR OIL / HIRSCHBOEK
o NV: 15-45 minutes
o Formation of a DIMPLING/ DROPLET like serum on the surface of the blood

b. STEFANINI
o 3-5 ml blood (37 oC)
o 1/2/16/18/24 hours
o NV: appreciable within 1 hour, complete within 18 to 24 hours

c. MacFarlane
o 5 mL of blood, 370C (1hour)
o NV= 44-67%
o CRT = Volume of serum

Question 5

Inflate BP cuff to a point hallway between the systolic and diastolic pressure (never exceed 100mmHg), maintain pressure for 5 minutes

A. platelet adhesiveness
B. clot retraction tine
C. capillary Fragility test
d. glass bead retention test

Answer 5

CAPILLARY FRAGILITY TEST/RUMPEL- LEEDE TEST/HISS METHOD / tourniquet test

❖ Inflate BP cuff to a point hallway between the systolic and diastolic pressure (never exceed 100mmHg), maintain pressure for 5 minutes

❖ Remove BP cuff and wait for 5 to 10 minutes before proceeding, count petechiae

❖ Positive test is found on THROMBOCYTOPENIA, decreased fibrinogen and in vascular purpura

Question 6

what is the grading in hiss method in Confluent petechiae on the arm and back of the hand

a. 1+
b. 2+
c. 3+
d. 4+

Answer 6

grade : 1+
Description : Few petechiae on the anterior part of the forearm
numbers of /petechiae : 0-10

G : 2+
Desc : Many petechiae on the anterior part of the forearm
NOP : 10-20

G: 3+
Desc : Multiple petechiae over the whole arm and back of the hand
NOP : 20-50

G : 4+
Desc : CONFLUENT petechiae on the arm and back of the hand
NOP : 50 and above

Question 7

What is the grade in Capillary fragility test if the number of petechiae is around 20-50

a. 1+
b. 2+
c. 3+
d. 4+

Answer 7

grade : 1+
Description : Few petechiae on the anterior part of the forearm
numbers of /petechiae : 0-10

G : 2+
Desc : Many petechiae on the anterior part of the forearm
NOP : 10-20

G: 3+
Desc : Multiple petechiae over the whole arm and back of the hand
NOP : 20-50

G : 4+
Desc : CONFLUENT petechiae on the arm and back of the hand
NOP : 50 and above

Question 8

An in vitro test for platelet adhesion

A. platelet adhesiveness
B. clot retraction tine
C. capillary Fragility test
d. glass bead retention test

Answer 8

GLASS BEAD RETENTION TEST
❖ An in vitro test for platelet adhesion

❖ Principle: as the anticoagulated blood passes through a glass bead column, normal platelets and vWF adhere to the glass bead surface that will result to a decrease in platelet count from the original platelet count.

❖ Uses 3 anticoagulated blood

PLATELET ADHESIVENESS (Salzmann) 
  ❖ Measure ability of platelets to adhere in glass surfaces

Question 9

decrease platelet retention test

a. VWD
b. BSS
c. Chediak higashi
d. gauchers disease

Answer 9

DECREASED PLATELET RETENTION 
 (GAndang gaBi ViCe)
  -  Glanzmann's thrombasthenia
  - aspirin administration
  - BSS
  - VWD
  - Chediak Higashi

Question 10

In vivo measure of primary hemostasis

A. platelet adhesiveness
B. bleeding time
C. capillary Fragility test
d. glass bead retention test

Answer 10

BLEEDING TIME ( Test for Platelet Adhesion)

❖ In VIVO measure of primary hemostasis

❖ The bleeding time test was first described by DUKE

❖ This test provides an ESTIMATE THE INTEGRITY of the platelet plug and thereby measures the interaction between the capillaries and platelets.

Question 11

As the platelet count drops below 100 × 109/L, the bleeding time will _________

a. remain
b. increase
c. decrease

Answer 11

As the platelet count DROP BELOW 100 × 109/L, the bleeding time INCREASE PROGRESSIVELY from a normal of 3 to 8 minutes to MORE THAN 30 minutes.

Question 12

In bleeding time this test it is performed by the blood is blotted in filter paper for every _______

a. 3 minutes
b. 30 minutes
c. 30 seconds
d. 5 seconds

Answer 12

The blood is blotted on the filter paper every 30 seconds

Question 13

which of the following method of bleeding time uses bf cuff

a. duke method
b. ivy method
c. rumpel leede method
d. modified template method

Answer 13

I. Duke method

  - skin puncture
  - 2 to 4 minutes

II. Ivy method
– uses BP cuff inflated at 40mmHg
-3 to 6 minutes

III, Modified template method
- 6 to 9 minutes

CAPILLARY FRAGILITY TEST/RUMPEL- LEEDE TEST/HISS METHOD
❖ Inflate BP cuff to a point hallway between the systolic and diastolic pressure (never exceed 100mmHg),