Specimen Collection and Handling

Question 1

Bevel up
15-30O angle
Length: 1 or 1.5 inch (Butterfly needle: ½ to ¾ inch)

Answer 1

Needle

Question 2

BP cuff as tourniquet

Answer 2

Inflate to 60 mmHg

Question 3

Disinfectant for ethanol testing

Dilution – 1:750

Answer 3

Benzalkonium chloride (Zephiran)

Question 4

Discontinue IV for 2 minutes
Collect sample below the IV site
Initial sample (5mL)  discard

Answer 4

IV line on both arms

Question 5

Increased:
Glucose (10% contam. w/ 5% dextrose  increased bld glucose by 500 mg/dL)
Chloride
Potassium
Sodium
Decreased:
Urea
Creatinine

Answer 5

IV fluid contamination

Question 6

Collected after a 3-day diet, from a peripheral vein

Answer 6

Renin blood level

Question 7

Early morning blood collection

12 hours after the last ingestion of food

Answer 7

Basal state collection

Question 8

1.75mm: preferred length to avoid penetrating the bone

Answer 8

Lancet

Question 9

<2.0mm (infants and children)

2-3mm (adults)

Answer 9

Incision (Skin puncture)

Question 10

Distance from the skin surface to bone or cartilage (middle finger)

Answer 10

1.5-2.4mm

Question 11

Best site for blood gas analysis (newborns

Answer 11

Indwelling umbilical artery

Question 12

Centrifugation requirement

Answer 12

1000-3000 RCF for 10 mins

Question 13

Increased:
“KLA6MP ITC2”
-K+
-LDH (150x)
-ACP
-ALP
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-Aldolase
-ALT
-AST
-Albumin
-Mg2+
-Phosphorus
-Iron
-Total protein
Affects bilirubin levels
Inhibits lipase

Answer 13

Hemolysis

Question 14

Required for: “ABCGLRP2”
Ammonia
Blood gases
Catecholamines
Gastrin
Lactic acid
Renin
PTH
Pyruvate
Decreased:
LD 4 and 5
Increased:
ALP

Answer 14

Refrigeration/Chilling

Low temp

Question 15

Bilirubin
Beta-carotene
Folate
Porphyrins
Vitamins A and B6

Answer 15

Photosensitive analytes

Question 16

Gel separator (SG: 1.04)
Serum: (SG: 1.03)
RBC: (SG: 1.05)

Answer 16

Thixotropic gel

Question 17

Increases potassium

Answer 17

Respinning gel tubes

Question 18

Additive: Buffered sodium citrate
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For ESR

Answer 18

Black top tube

Question 19

Additives:
None;
Na2EDTA
Sodium heparin

Answer 19

Royal blue top tube

Question 20

Additive: Thrombin

Answer 20

Orange top tube

Question 21

For glucose, BUN, ionized calcium, electrolyte studies (K+: best) and creatinine

Answer 21

Lithium heparin

Question 22

A.k.a. Mucoitin polysulfuric acid
Universal anticoagulant
Antithrombin
0.2 mg/mL blood

Answer 22

Heparin

Question 23

Weakly dissociated calcium component
2 mg/mL blood: anti-glycolytic
10 mg/mL blood: anticoagulant

Answer 23

Fluoride

Question 24

Chelation
1-2 mg/mL blood
Versene: disodium salt
Sequestrene: Dipotassium salt

Answer 24

EDTA

Question 25

Non-ionized form

3.2-3.8 g/dL (1:9 ratio)

Answer 25

Citrate

Question 26

Insoluble salt

1-2 mg/mL blood

Answer 26

Oxalate