Instrumentation Flashcards
For heating and sterilization
Borosilicate glasswares
Special alumina-silicate glass
Strengthened chemically than thermally
6x stronger than borosilicate
Corex (Corning)
For high thermal, drastic heat and shock
Can be heated to 900OC
Vycor (Corning)
Transport of quantity of analyte or rgt from one specimen rxn into another, and contaminating a subsequent one
Carry over
Multiple tests analyzed one after another on a given specimen
Sequential testing
System other than manufacturer’s reagents can be utilized for measurement
Open reagent system
One specimen
More than one test is analyzed
Parallel testing
All samples loaded at the same time
Single test is conducted on each sample
Batch testing
4 or 5 layers: -Spreading layer -Scavenger layer - Ascorbate Oxidase -Reagent layer -Indicator layer -Support layer Colored reaction Reflectance spectrophotometry
Thin-Film Analyzers
Dry slide technology
Most popular Req. vol: 2-6 μL Uses positive-displacement pipets Run multiple-tests-one-sample-at-a-time Random access capability (STAT)
ex: Vitros Dimension Dade Beckman ASTRA System (4 & 8) Hitachi Bayer Advia Roche Cobas Integra 800 Roche Analytics P Module
Discrete Analyzer
Acceleration and deceleration of the rotor
Advantage: Batch analysis
Centrifugal analyzer
Common reaction vessel
Air bubbles: separates and cleans
Glass coil: mix
Examples: “STS”
Simultaneous Multiple Analyzer (SMA)
Technicon Autoanalyzer II
SMAC
Continuous flow analyzer
Liquid: common reservoir –> dispense repeatedly
Dispenser/Dilutor pipet
Serological: w/ graduations to the tip (blowout)
Mohr: w/o graduations to the tip (self-draining)
Bacteriologic
Ball, Kolmer and Kahn
Micropipettes: <1 mL
Graduated or measuring pipet
Volumetric: for non-viscous fluid; self-draining
Ostwald folin: for viscous fluid; w/ etched ring
Pasteur: w/o consideration of a specific volume
Automatic macro-/micropipets
Transfer pipet