Specimen Collection/Analytical Methods Flashcards
What is the product of breaking down Amino Acids?
Ammonia
What does the Liver convert Ammonia to?
Urea
What happens to Urea during water reabsorption?
40%-60% is reabsorbed by the collecting ducts despite being freely filtered
What does the reabsorbed Urea contribute to?
A high osmolality
Describe the Enzymatic Method used to assay Urea
Urease hydrolyzes urea
Ammonium is quantified
340 nm
Color change
How can we collect a Urea specimen?
Plasma, Serum, or Urine
If using Plasma we CANNOT use Ammonium Heparin
Should be refrigerated
What is produced from the decomposition of Phosphocreatine?
Creatinine
Is Creatinine reabsorbed by the tubules?
NO
How do we measure Creatinine?
The Jaffe Reaction
Describe the Jaffe Reaction
Creatinine reacts with Picric acid to form a red-orange chromogen
What is the name of the alternative Jaffe Reaction?
Kinetic Jaffe Method
Define the Kinetic Jaffe Method
Serum and Alkaline Picrate are mixed
Absorbance is measured
Positive interference by a-keto acids and Cephalosporins
Negative interference by Bilirubin and Hemoglobin
What is the primary waste product of Purine metabolism?
Uric Acid
What happens to Uric Acid as it goes through the Nephron?
Gets filtered but then 98% - 100% is reabsorbed
Secreted at the Distal tubule
Only 6%-12% Is finally excreted
What are measurement of Uric Acid used to confirm/monitor?
Patients with Gout
Uric Acid Nephropathy
Kidney Dysfunction
