Specific Agglutinin Titer & Hemolysis Flashcards
reciprocal of highest dilution of the serum where antibody is still detectable
antibody titer
series of simple dilutions in which the concentration of a substance decreases by the same quantity in each successive step
Serial dilution
Many tiny agglutinates
w+
clear
*There are 3.
2+
3+
4+
few free cells
3+
hemolysis is used to?
- remove senescent (aged) or damaged RBCs in PB circulation
* recycling iron
may not be visible without microscope
w+
way of expressing concentration
titer
no free cells
4+
many medium-sized agglutinates
2+
One large agglutinate
4+
important in antibody titer determination
Serial dilution
Reagents in SAT determination
NSS (0.85-0.8%)
5% RCS of A1 cells
5% RCS of B cells
5% RCS of Rh (+) cells
several large agglutinates
3+
disintegration of the erythrocytes leading to hemoglobin release
hemolysis