Specific Agglutinin Titer & Hemolysis Flashcards

1
Q

reciprocal of highest dilution of the serum where antibody is still detectable

A

antibody titer

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2
Q

series of simple dilutions in which the concentration of a substance decreases by the same quantity in each successive step

A

Serial dilution

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3
Q

Many tiny agglutinates

A

w+

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4
Q

clear

*There are 3.

A

2+
3+
4+

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5
Q

few free cells

A

3+

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6
Q

hemolysis is used to?

A
  • remove senescent (aged) or damaged RBCs in PB circulation

* recycling iron

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7
Q

may not be visible without microscope

A

w+

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8
Q

way of expressing concentration

A

titer

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9
Q

no free cells

A

4+

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10
Q

many medium-sized agglutinates

A

2+

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11
Q

One large agglutinate

A

4+

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12
Q

important in antibody titer determination

A

Serial dilution

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13
Q

Reagents in SAT determination

A

NSS (0.85-0.8%)
5% RCS of A1 cells
5% RCS of B cells
5% RCS of Rh (+) cells

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14
Q

several large agglutinates

A

3+

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15
Q

disintegration of the erythrocytes leading to hemoglobin release

A

hemolysis

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16
Q

detects mixed field reactions & confirms a negative reaction

A

microscopic examination

17
Q

dark, turbid, homegenous

A

0

18
Q

rpm & time for hemolysis determination

A

1 minute @ 3,400 rpm

19
Q

expected result involving antigen & antibody reactions

A

hemolysis

20
Q

turbid ONLY

A

1+

21
Q

moderate number of free cells

A

2+

22
Q

no agglutinates

A

0

23
Q

dark & turbid

A

w+

24
Q

many free cells

A

1+

25
Q

Generally performed to differentiate pseudo-agglutination (rouleaux) from true agglutination

A

microscopic examination

26
Q

hemolysis is induced ___(1)___ through various physical agents such as:

a.
b.
c.
d.

A
  1. in vitro

a. heating
b. freezing
c. water
d. sound

27
Q

many small agglutinates

A

1+

28
Q

reagents used in hemolysis determination

A

NSS (0.85-0.9% NaCl)

washed PRBC

29
Q

rpm & time for agglutinin titer determination

A

15 seconds @ 3,400 rpm