Specific Agglutinin Titer & Hemolysis Flashcards
reciprocal of highest dilution of the serum where antibody is still detectable
antibody titer
series of simple dilutions in which the concentration of a substance decreases by the same quantity in each successive step
Serial dilution
Many tiny agglutinates
w+
clear
*There are 3.
2+
3+
4+
few free cells
3+
hemolysis is used to?
- remove senescent (aged) or damaged RBCs in PB circulation
* recycling iron
may not be visible without microscope
w+
way of expressing concentration
titer
no free cells
4+
many medium-sized agglutinates
2+
One large agglutinate
4+
important in antibody titer determination
Serial dilution
Reagents in SAT determination
NSS (0.85-0.8%)
5% RCS of A1 cells
5% RCS of B cells
5% RCS of Rh (+) cells
several large agglutinates
3+
disintegration of the erythrocytes leading to hemoglobin release
hemolysis
detects mixed field reactions & confirms a negative reaction
microscopic examination
dark, turbid, homegenous
0
rpm & time for hemolysis determination
1 minute @ 3,400 rpm
expected result involving antigen & antibody reactions
hemolysis
turbid ONLY
1+
moderate number of free cells
2+
no agglutinates
0
dark & turbid
w+
many free cells
1+
Generally performed to differentiate pseudo-agglutination (rouleaux) from true agglutination
microscopic examination
hemolysis is induced ___(1)___ through various physical agents such as:
a.
b.
c.
d.
- in vitro
a. heating
b. freezing
c. water
d. sound
many small agglutinates
1+
reagents used in hemolysis determination
NSS (0.85-0.9% NaCl)
washed PRBC
rpm & time for agglutinin titer determination
15 seconds @ 3,400 rpm
