special medias

Question 1

Mackonkey agar

Answer 1

selective and differential
selective against gram +(using bile salts and crystal violet)
lactose fermenters turn purple
non fermenters stay creamy
acid diffusion colors plate

Question 2

MSA

Answer 2

selective and differential
high salt
fermenters turn green
non fermenters stay red
detects staphylococcus aureus

Question 3

blood agar

Answer 3

tests for hemolytic activity(cells which burst red blood cells)(may be enhanced in low oxygen)
alpha is discolouration
beta is clearing
gamma is no hemolysis

Question 4

Media with high glucose

Answer 4

Saboraud Dextrose agar

sugar and acidic ph selects for yeasts and molds

Question 5

indole production

Answer 5

if trytophan is metabolized it will produce indole

indole detected by dripping kovacs reagent after incubation(turns red)

Question 6

Oxidase test

Answer 6

tests for cytochrome 3 oxidase(I think this lets you know if the organism can use oxygen to generate atp)
blue/purple = +
no colour = -