General

Question 1

Spectroscopy

Answer 1

the study of adsorption and emission of radiation

Question 2

Photometry

Answer 2

the measurement of intensity of radiation

Question 3

transmittance

Answer 3

the proportion of light that the sample allows to pass

Question 4

standard curve

Answer 4

a series of known samples used to generate comparison data

Question 5

adsorption spectrum

Answer 5

• Measure a sample at
various wavelengths and
plot a curve of the
absorbance

• Useful to determine the
optimal wavelength for
measuring Abs

Question 6

bradford assay

Answer 6

measurement of color change when coomassie blue die binds proteins

Question 7

Biuret Assay

Answer 7

measurement of color change when Copper II sulfate reacts with proteins under alkaline conditions

Question 8

gram + bacteria

Answer 8

multiple layers of peptidoglycan(80 nm)
techoic acids aid in keeping the layers together
- also increase the - charge of the cell wall

Question 9

gram - bacteria

Answer 9

single layer of peptidoglycan(~8 nm thick)

second phospholipid membrane outside the peptidoglycan

Question 10

negative stain

Answer 10

Allows visualization of glycocalyx

• uses nigrosin
• cells not fixed
• thin film preparation

Question 11

leifson stain

Answer 11

crystal violet dye + mordant

used to see flagellum