Southern Blotting Hybridization

Question 1

____________ developed this technique in the mid-1970s

Answer 1

Edward Southern

Question 2

The detection of specific DNA in samples

Answer 2

Southern Blotting

Question 3

Step 1: Digest DNA with _______________________

Answer 3

restriction endonuclease

Question 4

Step 2: Perform ___________ on DNA fragments from different digests.

Answer 4

gel electrophoresis

Question 5

Step 3: DNA fragments fractioned by size, visible under UV light if gel is soaked in ________________

Answer 5

ethidium bromide

Question 6

Step 4: transfer (blot) gel to nitrocellulose filter using _____________

Answer 6

southern blot technique

Question 7

Step 5: _________ bind to filler, mirroring gel positions.

Answer 7

DNA fragments

Question 8

Step 6: Hybridize filler with ______________

Answer 8

radioactive labeled probe

Question 9

Step 7: Expose filter to X-ray film, revealing ________________

Answer 9

hybridized DNA fragments.

Question 10

Northern Blotting detects what?

Answer 10

RNA

Question 11

Southern blotting detects what?

Answer 11

DNA

Question 12

Eastern blotting detects what?

Answer 12

Protein :3