Cloning Vector Flashcards
Serves as a vehicle that transports foreign DNA into host cells
Cloning Vector
A molecule in which foreign DNA can be inserted to produce clones of recombinant DNA
Cloning Vector
Where the trait is located
Gene locus
DNA replication occurs in?
Nucleus
ORI (site) stands for?
Origin of replication initiation
The starting point of DNA replication
ORI site
Cloning vectors should be _________ for efficient transformation and stable maintenance of inserted DNA.
small enough
A cloning vector should have unique ____________ where it will be cut.
restriction sites
____________ are used to determine wanted genes, for them to be able to survive. These are mostly antibiotic resistance genes.
selectable markers
Original double-stranded DNA remains intact, while a completely new copy is synthesized.
Conservative replication
DNA strands break and rejoin in a scattered manner, resulting in segments of original and newly synthesized DNA.
Dispersive replication
Each strand of the original DNA molecule serves as a template for the synthesis of a new complementary strand.
Semi-conservative replication
The three models of replication are:
Conservative, Dispersive, Semi-conservative
Prokaryotes have ___ site of replication
only one
Eukaryotes have ________ of replication.
multiple sites