Cell Transformation Flashcards
A key step in DNA cloning, that occurs after restriction digest and ligation
Cell transformation
Which method uses liposomes to carry DNA into cells, exploiting lipid bilayers?
Lipofection
Factors that lead to uncontrolled growth are called?
Mutations
Cells with no walls
Protoplast
In this process, protoplasts are exposed to short electrical pulses that introduce openings where DNA molecules enter.
Electroporation Cell Process
Uses a gene gun shot at a high velocity that penetrates cell wall.
Biolistic / Microprojectile Bombardment
DNA is injected directly to the nucleus, and is then integrated into the chromosome.
Microinjection
______ is used to transfer DNA into animal cells
Virus
____ serves as a buffer.
salt solution
______ is a solution that helps maintain a stable pH level by resisting changes in acidity or alkalinity
buffer