Small Regulatory Noncoding RNA's

Question 1

Detail miRNA

Answer 1

They are 21 nt long, were first discovered in the worm and then in eukaryotes, they derive from cellular genes, are involved in post transcriptional regulation and sequence specific regulation. They bind imperfectly with their target, they are conserved across evolution, they are tissue specific. They are single stranded RNA

Question 2

Ago2

Answer 2

has catalytic function, silencer activity

Question 3

miRNA can act as proto-oncogenes or tumor suppressors

Answer 3

true

Question 4

how are miRNA used as biomarkers

Answer 4

because all body fluids contain miRNA, they are more stable than mRNA, and they are specifically mutated in each disease

Question 5

how are miRNA used as therapeutics

Answer 5

they can be used for miRNA reduction or miRNA replacement, right now the most widely used approach to regulate miRNA levels in vivo is using anti-miR (inhibitors)