Skill: Blood Smear Flashcards
Eosin
Red-orange stain or dye
Fixative
Preservative
Methylene blue
Blue stain or dye
Wright’s stain
Polychromatic stain, mix of methylene blue, eosin, and methanol
Step 1 of preparing a blood smear (as listed on the hosa webite)
Assemble materials
Step 2: After assembling materials…
Wash hands
Step 3: After washing hands…
Put on PPE
Step 4: After applying PPE…
A small drop of blood is placed on the slide about 3/4 inches from the end
Step 4 and 5: Place blood at correct size
Step 6 and 7: …
Place edge of coverslip or spreader slide in front of the blood slide, and place it at a 30 to 35 degree angle
Step 8, 7, 9, 10, 11, and 12: After positioning the spreader…
Drag the spreader back until it reaches the blood, let the blood spread to the edges of the spreader, and push the blood to the opposite end of the slide
Step 13: After smearing…
Dispose of the coverslip or spreader in the sharps container
Step 14 and 15: After disposing of spreader…
Allow slide to air dry, and let the judge look at it
Step 16: After the judge looks at the smear…
Label the slide with patient’s name, doctor’s name, and ID number
Step 17: After labeling the slide…
Clean and replace all equipment
Step 18: After cleaning and replacing equipment…
Use disinfectant to wipe counter and area
