Simple Stain Flashcards
Why is it necessary to heat fix bacterial cells?
Helps adherence
Common stain in simple staining?
Crystal Violet
2 reasons for cooling slide before applying stain?
1) Prevents cracking
2) Prevents precipitate of stain from forming on slide
Stain used for negative stain is?
Nigrosin
Easiest staining procedure is?
Simple Stain
Why is stain valuable?
It identifies organisms
What type of mount will you use in the lab to study microorganisms for a short period of time?
Temporary Mount
Methylene blue
Simple Stain, basic dye
Crystal violet
simple stain, basic dye
Safranin
simple stain, basic dye
Basic dyes….
stain nucleic acid and elements that are acidic
Fuschin
Acid Dye
Acid dyes…
stain cytoplasmic components
Simple stain is a _______ step staining procedure in which only one dye is used.
ONE
For a structure in bacteria
Selective Stain
To distinguish, classify and identify bacteria.
Differential Stain
Ex: Gram Stain, Acid Fast Stain
Differential Stain
Capsules
Selective Stain
Flagella
Selective Stain
Spores
Selective Stain
Metachromatic granules
Selective Stain
Fat bodies
Selective Stain
Makes light structures dark, dark structures light.
Negative stain
Ex: Nigrosin
Negative Stain
