Simple Stain

Question 1

Why is it necessary to heat fix bacterial cells?

Answer 1

Helps adherence

Question 2

Common stain in simple staining?

Answer 2

Crystal Violet

Question 3

2 reasons for cooling slide before applying stain?

Answer 3

1) Prevents cracking

2) Prevents precipitate of stain from forming on slide

Question 4

Stain used for negative stain is?

Answer 4

Nigrosin

Question 5

Easiest staining procedure is?

Answer 5

Simple Stain

Question 6

Why is stain valuable?

Answer 6

It identifies organisms

Question 7

What type of mount will you use in the lab to study microorganisms for a short period of time?

Answer 7

Temporary Mount

Question 8

Methylene blue

Answer 8

Simple Stain, basic dye

Question 9

Crystal violet

Answer 9

simple stain, basic dye

Question 10

Safranin

Answer 10

simple stain, basic dye

Question 11

Basic dyes….

Answer 11

stain nucleic acid and elements that are acidic

Question 12

Fuschin

Answer 12

Acid Dye

Question 13

Acid dyes…

Answer 13

stain cytoplasmic components

Question 14

Simple stain is a _______ step staining procedure in which only one dye is used.

Answer 14

ONE

Question 15

For a structure in bacteria

Answer 15

Selective Stain

Question 16

To distinguish, classify and identify bacteria.

Answer 16

Differential Stain

Question 17

Ex: Gram Stain, Acid Fast Stain

Answer 17

Differential Stain

Question 18

Capsules

Answer 18

Selective Stain

Question 19

Flagella

Answer 19

Selective Stain

Question 20

Spores

Answer 20

Selective Stain

Question 21

Metachromatic granules

Answer 21

Selective Stain

Question 22

Fat bodies

Answer 22

Selective Stain

Question 23

Makes light structures dark, dark structures light.

Answer 23

Negative stain

Question 24

Ex: Nigrosin

Answer 24

Negative Stain