Microscopes Flashcards
Used routinely
Bright field microscope
Stained microorganisms are observed
Bright field microscope
Organisms appear dark against a bright background
Bright field microscope
Organisms appear bright against a dark background
Dark field microscope
An opaque stop is inserted below the condenser, or the stop is built into the condenser.
Dark field microscope
Only peripheral rays of light enter the condenser; the rays pass through the specimen at an angle, not directly.
Dark field microscope
Used to examine organisms that are hard to stain. Ex: Treponema (syphilis causing organism)
Dark field microscope
Used to observe: bacteria in tissue.
Phase microscope
Used to observe: parasites in clinical material
Phase microscope
Used to observe: inclusion bodies in virus infected material.
Phase microscope
Used to observe: histological preparations
Phase microscope
Works on the principle of refractive index
Phase microscope
Gives a greater resolution.
Ultraviolet Microscope
ability to reveal fine detail it uses UV light which is of shortwave length
Ultraviolet Microscope
You can obtain 2-3x greater magnification compared to visible light.
Ultraviolet Microscope
A photographic plate is used to record images.
Ultraviolet Microscope
A quartz lens is used
Ultraviolet Microscope
UV light is used, but microorganisms are treated with fluorescent dye or a dye-antibody complex that causes them to fluorese.
Fluorescence Microscope
Execellent for detecting treponemal antibody for syphillis.
Fluorescence Microscope
This procedure is more sensitive and specific.
Fluorescence Microscope
Used in research, virology and cytology.
Electron Microscope
An electron stream is used instead of light
Electron Microscope
Uses a magnet instead of a lens
Electron Microscope
Only nonliving material can be studied.
Electron Microscope
Specimens require special preparation techniques
Electron Microscope
Images are observed on a fluorescent screen through a window at the base of the column.
Electron Microscope
A photographic plate can be inserted to the recorded image, called an electronmicrograph.
Electron Microscope
Coarse adjustment knob can only be used in…
low power
Fine adjustment knob can only be used in…
Low, high and oil immersion.
In our lab, we use the __________ microscope.
Compound binocular light microscope.
The ability of a microscope to reveal fine detail is known as…
resolution.
Another name for the 100X lens is…
oil immmersion
The letter E is moved to the right. In which direction does it appear to move in the field of view?
Left
What part of the microscope regulates the amount of light entering?
The Condenser
What part of the microscope is used if some focusing adjustment is needed in high power?
Fine Adjustment Knob
Who first observed microorganisms?
vanLeeuwenhoek
What cannot be seen with the compound microscope?
viruses
