Microscopes

Question 1

Used routinely

Answer 1

Bright field microscope

Question 2

Stained microorganisms are observed

Answer 2

Bright field microscope

Question 3

Organisms appear dark against a bright background

Answer 3

Bright field microscope

Question 4

Organisms appear bright against a dark background

Answer 4

Dark field microscope

Question 5

An opaque stop is inserted below the condenser, or the stop is built into the condenser.

Answer 5

Dark field microscope

Question 6

Only peripheral rays of light enter the condenser; the rays pass through the specimen at an angle, not directly.

Answer 6

Dark field microscope

Question 7

Used to examine organisms that are hard to stain. Ex: Treponema (syphilis causing organism)

Answer 7

Dark field microscope

Question 8

Used to observe: bacteria in tissue.

Answer 8

Phase microscope

Question 9

Used to observe: parasites in clinical material

Answer 9

Phase microscope

Question 10

Used to observe: inclusion bodies in virus infected material.

Answer 10

Phase microscope

Question 11

Used to observe: histological preparations

Answer 11

Phase microscope

Question 12

Works on the principle of refractive index

Answer 12

Phase microscope

Question 13

Gives a greater resolution.

Answer 13

Ultraviolet Microscope

Question 14

ability to reveal fine detail it uses UV light which is of shortwave length

Answer 14

Ultraviolet Microscope

Question 15

You can obtain 2-3x greater magnification compared to visible light.

Answer 15

Ultraviolet Microscope

Question 16

A photographic plate is used to record images.

Answer 16

Ultraviolet Microscope

Question 17

A quartz lens is used

Answer 17

Ultraviolet Microscope

Question 18

UV light is used, but microorganisms are treated with fluorescent dye or a dye-antibody complex that causes them to fluorese.

Answer 18

Fluorescence Microscope

Question 19

Execellent for detecting treponemal antibody for syphillis.

Answer 19

Fluorescence Microscope

Question 20

This procedure is more sensitive and specific.

Answer 20

Fluorescence Microscope

Question 21

Used in research, virology and cytology.

Answer 21

Electron Microscope

Question 22

An electron stream is used instead of light

Answer 22

Electron Microscope

Question 23

Uses a magnet instead of a lens

Answer 23

Electron Microscope

Question 24

Only nonliving material can be studied.

Answer 24

Electron Microscope

Question 25

Specimens require special preparation techniques

Answer 25

Electron Microscope

Question 26

Images are observed on a fluorescent screen through a window at the base of the column.

Answer 26

Electron Microscope

Question 27

A photographic plate can be inserted to the recorded image, called an electronmicrograph.

Answer 27

Electron Microscope

Question 28

Coarse adjustment knob can only be used in…

Answer 28

low power

Question 29

Fine adjustment knob can only be used in…

Answer 29

Low, high and oil immersion.

Question 30

In our lab, we use the __________ microscope.

Answer 30

Compound binocular light microscope.

Question 31

The ability of a microscope to reveal fine detail is known as…

Answer 31

resolution.

Question 32

Another name for the 100X lens is…

Answer 32

oil immmersion

Question 33

The letter E is moved to the right. In which direction does it appear to move in the field of view?

Answer 33

Left

Question 34

What part of the microscope regulates the amount of light entering?

Answer 34

The Condenser

Question 35

What part of the microscope is used if some focusing adjustment is needed in high power?

Answer 35

Fine Adjustment Knob

Question 36

Who first observed microorganisms?

Answer 36

vanLeeuwenhoek

Question 37

What cannot be seen with the compound microscope?

Answer 37

viruses