Gram Stain

Question 1

A culture in which you have many different types are associated together is considered to be…?

Answer 1

Mixed Culture

Question 2

The single most important bacteriological stain is…

Answer 2

Gram’s Stain

Question 3

Counterstain used in Gram Stain?

Answer 3

Safranin

Question 4

Decolorizing agent in Gram Stain?

Answer 4

95% Ethyl Alcohol

Question 5

Gram negative bacteria stain what color?

Answer 5

red

Question 6

Gram Positive bacteria stain what color?

Answer 6

Purple

Question 7

Most critical part of the gram staining procedure is?

Answer 7

Decolorization

Question 8

Technique used to maintain sterility and avoid contamination is called..?

Answer 8

Aseptic Technique

Question 9

Turbidity can best be seen on…

Answer 9

Broth Tube

Question 10

What is the purpose of flaming your loop?

Answer 10

Sterilization

Question 11

4 Steps of Gram Stain

Answer 11

1) Crystal Violet
2) Iodine
3) 95% Ethyl Alcohol
4) Safranin

Question 12

Crystal Violet is the…

Answer 12

Stain

Question 13

Iodine is the…

Answer 13

Mordant

Question 14

95% Ethyl Alcohol is the…

Answer 14

Decolorizer

Question 15

Safranin is the…

Answer 15

Counterstain

Question 16

Agar in tubes…

Answer 16

Agar Slants

Question 17

Agar in plates…

Answer 17

Plate Cultures

Question 18

Liquid in tubes (cloudy or turbidity; fluid)

Answer 18

Broth Cultures

Question 19

Made of Gelatin

Answer 19

Stabbs Culture

Question 20

Enhances color of dye…

Answer 20

Mordant