simple Flashcards

1
Q

parts of the mouth

A
labarum
mandible
maxillae 
labium
proboscis
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2
Q

what’s proboscis made of and length

A

tongue + palpi labiales + maxillae

worker 6.7mm, drone 4mm, queen 3.5mm

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3
Q

compound eyes

A
  • 2
  • perceive movement, colour, patterns and polarised light
  • made up of many ommatidia
  • divided: light, sensory, nerve an dbrain
  • hairs are present around surface edge
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4
Q

simple eyes

A
  • 3
  • drone, relocated on the front of the fae
  • lens, beneath them are transparent cells and below light sensitive cells then random and nerve fibres
  • function in perception of differences in light intensity
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5
Q

antenna

A
  • two long chitin tubes located on front side of head
  • queen + worker = 12 segments, drone = 13
  • funiculus, pedicures and scapus
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6
Q

exoskeleton

A
  • basement membrane
  • epidermis
  • cuticle: endocuticle (chitin), exocuticle (chitin+scletrotin), epicuticle (sclerotin)
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7
Q

front legs of workers

A
  • base of 1st segment of tarsus is semi-circular notch containing stiff bristles forming a comb
  • near to distal end of tibia = fibula
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8
Q

parts of leg

A
coxa
trochanter
femur
tibia
tarsus
pretarsus
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9
Q

wings

A
  • 2 pairs attached to 2nd and 3rd segment of thorax
  • made of thin cuticle, thickened veins
  • hooked in flight, independent at rest
  • newly hatched, veins are pumped full of haemolymph
  • in flight = figure of 8 motion
  • when resting, w ing can be folded over back
  • queen has shorter wings than abdomen, worker same drones longer
  • hind wing is smaller and more rounded
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10
Q

sting

A
  • only females
  • cavity within abdomen segment 7
  • shaft = paired lancets and 1 stylet
  • stylet on dorsal side of lancets and end is swollen into bulb
  • venom sac empties into bulb
  • poison glands are long extensions of sac
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11
Q

hind legs of workers

A
  • outside of tibia is slightly concave + fringed
  • pollen basket/corbicula
  • distal inner of tibia is rostellumrake
  • dorsal hind of 1st tarsus = auricle, teeth pointing upwards
  • hairs arranged in rows of 10 on medial side of 1st (pollen brush)
  • rostellum + auricle = tibia-tarsal joint (pollen press)
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12
Q

acarosis

A
  • tracheal mite
  • affects adults
  • live and reproduce mainly in large prothoracic trachea
  • feeds on haemolypmh piercing through tracheal wall
  • early spring
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13
Q

morphology of acarosis

A

oval shape, whitish colour, 4 pairs of legs

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14
Q

life cycle of acarosis

A
  • female enters trachea of young bee before chitin valve solidifies
  • female lays 8-20 eggs
  • development takes 2 weeks
  • mating takes place in trachea
  • mated females leave and infest other bees
  • males stay in trachea
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15
Q

treatment and control of acarosis

A
  • no authorised VMPs
  • using volatile chemicals (formic and menthol)
  • OIE listed
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16
Q

chalkbrood

A
  • fungal disease of capped brood
  • young bee larvae are infected through ingestion of food contaminated with spores
  • spores germinate in the gut, penetrate peritrophic membrane and gut epithelium, enabling mycelium to grow inside
  • 1st sign - white and black mummies
  • pupae are resistant, death occurs in capped stage
  • dead larvae at first pale yellow, soft and covered with fluffy white mycelium
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17
Q

prevention and control of chalkbrood

A
  • proper ventilation
  • old comb replacement
  • regular requeening
  • varoosis control
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18
Q

stonebrood

A
  • fungal disease of capped brood and adults
  • zoonosis
  • infection by contaminated feed or by penetration of cuticle
  • mycelium grows inside > larvae tissues > larvae changes to hardened mummies > muscles and intestine of adult bees become hardened and die
  • prevention: good beekeeping practice
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19
Q

american foulbrood

A
  • infectious and contagious, brood
  • cause d by bacteria
  • notifiable disease
  • spores germinate into vegetative spores in larvae mid-gut
  • dead larvae: without any shape, brown, ropey theads
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20
Q

clinical signs of American foulbrood

A
  • brood frame appeared mottled, unregular formation of capped brood
  • concave, punctured and mostt capping
  • dead, dark brown and larvae mass
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21
Q

European foulbrood

A
  • infectious and contagious
  • uncapped brood
  • non-spore forming bacterium
  • infected larvae die - 4/5days old
  • notifiable disease
  • puffy with visible tracheal system
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22
Q

controlling European foulbrood

A
  • removing of diseased brood

- regular queen exchange

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23
Q

nosemosis

A
  • parasitic disease of adults
  • affect epithelial cells of mid gut
  • Type A and Type C
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24
Q

Type C nosemosis

A
  • emerging disease
  • asymptomatic: induce colony wearing
  • atrophy of hypo pharyngeal glands
  • suppresses immunity
  • no seasonal pattern
  • phase 1,2,3,4 (asymptomatic, replacement, recovery and depopulation)
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25
Q

chronic bee paralysis

A
type 1
- greater number, have bloated abdo
- honeybees get out of hives in groups
- trembling winds and body, ataxia
- inability of flying, crawling on ground
type 2
- smaller number, bees are smaller
- become hairless and appear dark and shiny
- crawling and body and wings trembling
- with shortened abdomen
- black and smaller look like flies
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26
Q

sac brood virus

A
  • infectious disease of larvae within capped brood and young adults
  • infected larvae fail to pupate after cell capping and die
  • infected adult bee changes behaviour: stops eating pollen, foragers earlier in life, secretion of royal jelly is impaired
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27
Q

acute bee paralysis

A
  • become pathogenic following its direct injection into honeybee haemolymph by V. destructor
  • clinical signs: bees walking around in groups, position of wings is abnormal, colony weakining
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28
Q

salivary gland

A
  • Two parts = thoracic + post cerebral
  • developed in larvae – excrete yarn for building cocoon
  • adults excrete alkaline saliva, moistening solid food
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29
Q

hypopharyngeal gland

A
  • Developed only in workers
  • Young bees (8-12days) they produce royal jelly
  • Later, HPG produces enzymes: invertase, glucose-oxidase (converting nectar to honey)
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30
Q

alimentary

A
  • Foregut (pharynx, oesophagus, crop/honey stomach, proventriculus)
  • Midgut (digestion place)
  • Hindgut (S. I, rectum)
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31
Q

spiracles

A
  • 10 pairs on each segment
  • Equipped with valves, allows them to be closed at time to prevent dehydration
  • Surrounded by hairs
  • Abdominal spiracles have entrances = atrium
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32
Q

trachea

A
  • Tubes leading from spiracles; join together to give two lateral trachea running down the sides of the body
  • Traches are lined with cuticle (chitin)
  • Look like coiled springed wrapped rounded
  • Taenidium = single turn of spiral
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33
Q

air sacs

A
  • Have thin walls allowing to expand or collapse

* Present in head, thorax and abdomen

34
Q

circulatory

A
  • Two thin diaphragms, contain muscle fibres and their contraction in sequence
  • Heart is in tube, closed at back and with 5 pairs of openings (ostia)
  • Function = propel haemolymph forward and into aorta-pressure inside heart causes ostia to close
35
Q

wax glands

A
  • 4 pairs, located within 4 sterna of 4-7 abdominal sclerites
  • Each pair is situated to the front of its sternum so that the sclerites overlap
  • Below each wax gland = wax mirror
  • Oenocytes = produce wax
36
Q

nervous system

A
  • CNS + nerve + sense organs
  • Brain + sub-oesophageal ganglion + segmental ganglia (7) and ventral nerve cord
  • Brain divided into: 1. Optic lobes and corpora pedunculata, 2. Deutocerebrum, 3. tritocerebrum
37
Q

midgut layers

A
  • Circular and longitudinal muscle coat
  • Basement membrane
  • High cylindrical epithelial cells
  • Epithelium is dragged with special layer – rabdhorium (linked to other cells) – modification of upper plasmatic coat (apical part)
  • Rabdhorium is partially disconnected and modificated in peritrophic membrane which encompassed food, acts as membrane which enables flow through digestive juices
38
Q

mandibular gland

A
  • Situated on base of upper maxillae
  • Developed in queens and workers
  • Melts wax, propolis, pollen grain
  • Defence pheromone
  • Role in ingestion and comb building
39
Q

deformed wing virus

A
  • Linked with high V. destructor infestations
  • Clinical signs: deformities
  • Horizontal and vertical transmission
  • Wings are deformed, stubby and useless, rounded and shortened abdomen with miscolouring
  • Colony weakened
40
Q

black queen cell virus

A
  • In intensive rearing of honeybee queens
  • Closely associated with Nosema spp
  • Cells with infected larvae develop dark brown or black cell walls
  • Disease pre-pupa or pupae cannot develop into adult queen and die
41
Q

varroa destructor

A
  • Obligatory parasitic mite
  • Larvae, pupae and adult honeybees
  • Reproduces inside the capped bee brood
  • Reproduces only in drone brood
42
Q

morphology of varroa destructor

A

Female morphology
• Oval, flattened body, covered with hairs
• Four pairs of legs, 1st pair directed forward
• Brown-reddish sclerotised shield
• Mouthparts (gnathostome)
Male morphology
• Smaller, round in shape, soft body, lightly coloured
• Can only be found in capped brood
• Not ablet o survive and feed on adult bees
• Die inside brood soon after mating

43
Q

life cycle of V. destructor

A

Phoretic phase
• Mature female mite present on adult bee
Reproductive phase - Inside bee brood

44
Q

assessment of v. destructor

A
  • “sugar shake” method, 250-300 bees  2 spoons of powdered sugar  shake for 1 min  leave for 3-5 min  invert and shake over a white board
  • Drone brood uncapping method
45
Q

control of V. destructor

A

• VMPs (except formic acid)

46
Q

small hive beetle

A
  • Parasite and pest
  • Need early diagnosis
  • Life cycle is connected with soil
  • lays eggs in irregular clusters
  • Club shaped antennae
  • 2 pairs of winter – inner softer, outer harder (elytra)
  • They remove bees eggs
47
Q

small hive beetle larvae

A
  • Feeds on honey, pollen honeybee brood
  • White to cream; cephalic capsule is brown
  • No pseudopods on ventral side
  • 1cm long, segmental body, 3 pairs of legs when it’s wandering larvae
  • Differential diagnosis: wax mouth
48
Q

controlling small hive beetle

A
  • Coumaphos treatment

* 42- 45 days can stay in the hive

49
Q

life cycle of small hive beetle

A

Adult goes to brood  mated female comes in hive  eats everything, leaving their own brood/eggs  eggs to wandering larvae (1-014 days)  release enzymes that ferments ripe honey  liquid honey in colony  wandering larvae leave, dig the soil  don’t eat, pupa and metamorphosis in adult beetle in soil  male and female mate under soil  leave and find new hive

50
Q

tropilelosis

A
  • Different shape and smaller than varroa
  • Use PCR to distinguish species
  • Not able to survive without brood in hive more than 2 days
51
Q

morphological identification of tropilelosis

A
  • Oblong, non-segmented body
  • 4 pairs of legs, 1st pair directed as antennae
  • Reddish-brown colour of body
52
Q

diagnostic procedure of tropielosis

A
  • Differential diagnosis: varroa, B. coeca
  • Collection of mites from honeybee sample + capped brood
  • Sample: collect mites fixed in 70% alcohol or freeze at -20C, cover document and mark
  • Never send live mites to lab
53
Q

hives

A
  • Structure used by honeybees for home
  • Hives with non-moveable frames (combs)
  • Transition hive form (move partially)
  • Hives with moveable frames
54
Q

traditional hive

A
  • built from different natural materials
  • Wax combs are fixed on internal wall of hive
  • Volume of hive is small
  • Have to use knife to remove frame
  • +ve: cheap, small, fast and often swarming
  • -ve: poor biological and technological insight, small volume and squeezed honey
55
Q

layen’s hive

A
  • Horizontal
  • +ve: brood nest space could be enlarged
  • -ve: heavy for transportation
56
Q

comb

A

Wax portion of colony in which eggs are laid, brood reared and honey and pollen stored.
3 types of cells: worker, drone and queen

57
Q

caps above brood

A

dry, porous, colour of around comb cells, convex (worker less, drone more)

58
Q

caps above ripe honey

A

plain, sleek, glossy

59
Q

comb foundation

A
  • Artificial structure - thin sheets of beeswax with outlines of cell bases
  • Bees then construct a complete comb
  • Combs wired or unwired
  • Take 30-35% of combs each year and melt them and dark = old
60
Q

langstroth-root hive

A
  • Vertical
  • Two or more boxes
  • +ve: all parts are standard construction, suitable for modern beekeeping, possibility for volume increasing
  • no disadvantages
61
Q

listing hive- alberti-znidarsic hive

A
  • Vertical
  • Compact box with ventilation opening
  • Brood compartment and separated by queen excluder in grid form
  • +ve: successful colony overwintering
  • -ve: impossibility for volume increase, expensive and complex construction
62
Q

dadant-blatt

A
  • vertical
  • two or more boxes
  • +ve: volume of hive is possible to increase by adding boxes
  • -ve: different heights of hive, compartments aren’t possible to use lower boxes for brood chamber
63
Q

sterilisation of wax

A

120-125C under pressure of 1-2 bars without adding water and minimum 30 minutes

Liquid wax is cooled down on hexagonal sheets and will form the comb formation

64
Q

trophallaxis

A
  • One worker begs for food, other offers
  • Begging bee pushes proboscis towards mouth of another, open mandible and push proboscis forward and regurgitates
  • Bee makes contact their antennae also touch
65
Q

pheromones

A
  • Continuous information flow between individuals
  • Produced in exocrine gland
  • Substances that are secreted to the outside by an individual and received by a second individual of the same species
  • Nasonov and alarm pheromones
66
Q

round dance

A

Used when there’s source of food near hive

67
Q

dorso ventral abdo dance

A

Used when more foragers need to be recruited during nectar flow

68
Q

piping

A

Associated with queens around the swarming; vibration sounds generated by queen; still closed in queen cell or free in hive

69
Q

queen reproduction

A

Ovaries, oviduct, spermatheca, poison gland and venom sac

70
Q

drone reproduction

A

2 testis, mucus gland, seminal vesicles, endophalus

71
Q

mating “wedding flight:

A
  • Spring, early summer
  • Queen mating mood = going out of hive (3-6days of life)
  • Mating (6-10 days)
  • Drone mating mood (after 10 days)
  • Queens fly to drone congregation area
  • Drone approaches queen from behind, everts endophalus into open cloaca
  • Flips over backwards and endophalus breaks off, inside queen, drones fall
  • After 48-64hrs moves into spermatheca
72
Q

swarming

A
  1. Developing of drone brood
  2. Start of building queen cells (10-20)
  3. Queen is on diet by reducing food supply, egg laying reduces
  4. Swarming about 7-8 days before 1st new queen emerge
  5. Next 24 days collect swarm and move into new hive
73
Q

figure of 8 dance

A

Forage is >100m away from hive, bee buzzes its wings and rapidly shakes its abdomen from side to side, it’s performed on vertical face of comb (direction of sun)
Distance is also communicated by length of straight run

74
Q

suspicion of intoxication

A
  • Absence of major infectious disease
  • Symptoms in most hives
  • Clinical signs: neurological issue, population decrease, lack brood, bee mortality
75
Q

sample for intoxication

A

• Sample of symptomatic/freshly dead adult honeybees per apiary
• Min 500 honeybees or individual sample from each hive 50g,
• Packed in marked paper bag + plastic bag
• Storage -20C
Individual sample for diseases examination (60 adult bees, packed and marked and cover document)

76
Q

hard acaphicides

A
  • Amitraz, flumethrin, apistan
  • Strips
  • Chronic treatment
77
Q

coumphaos

A
  • Organophosphate

* Strips for 6 weeks

78
Q

intoxication outcomes

A

Acute
• Death after single exposure
• Intoxication symptoms
• Pyrethroids rapidly induce neurotoxicity in adult bees
• Organophosphorus components cause hyperexcitable and irritable behaviour
Sublethal
• Individuals survive after intoxication and transfer to brood, house and queen
• Poisoning with neonicotinoids impair: cognitive, thermoregulation + immune
Chronic
• After chronically exposure to chemicals and their residues
Symptoms
• Disorientation, paralysis, regurgitation and diarrhoea

79
Q

soft acphacides

A
  • Thymol
  • Under 30C, otherwise toxic (15-30C)
  • Best 20-25C
80
Q

EU legislative framework on pesticides

A
  • Sustainable use of pesticides
  • Establishing maximum residue levels of pesticide
  • Collection of statistics
  • Technical requirement for machinery