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1
Q

3c) Resistance to neonicotinoid pesticides has been observed in some insects. Describe briefly how this resistance could have arisen in populations of insects. (2) (may 2016)

A

Mutation for resistance in some insects
Mutation causes breakdown of pesticide/changes for receptor site
Natural selection for resistance

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2
Q

4b) Starting from the concept of gene pool, explain briefly how populations of early vertebrates could have evolved into different groups. (3) (may 2016)

A

Gene pool is all genes
Geographical isolation
Speciation/gene pool splits if populations are reproductively isolated
In different environments there are different selection pressures
Alleles frequency diverge

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3
Q

4c) Mitochondria are thought to have evolved from prokaryotic cells. Describe two adaptations of the mitochondria, each related to its function. (2) (may 2016)

A

small intermembrane space for a gradient «of protons» to develop

matrix contains enzymes for Krebs cycle/link reaction

ribosomes/DNA for protein synthesis/replication

cristae maximize surface area for ATP synthesis/chemiosmosis/proton pumping/electron transport chains

ATP synthase/stalked particles generates ATP from ADP + Pi

electron transport chains for generating a proton gradient/for releasing energy from reduced NAD

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4
Q

5a) Outline the action of enzymes. (4) (may 2016)

A
catalyse/speed up reactions 
substrate-specific 
lower the activation energy «of a chemical reaction» 
substrate binds to active site 
enzyme–substrate complex formed
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5
Q

6b) Outline the effects of putting plant tissue in a hypertonic solution. (4) (may 2016)

A

hypertonic solution has higher solute concentration than cells
water moves out of the cells by osmosis «into the hypertonic solution»
water moves from lower to higher solute concentration
pressure inside cell drops/cell no longer turgid
volume of cytoplasm drops

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6
Q

7a) Things to include on a eukaryotic plant cell seen in an electron micrograph. (4) (may 2016)

A
cell wall
plasma membrane
vacuole 
mitochondria
chloroplast
Nucleus
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7
Q

7b) Outline how the energy flow through food chains limits their length. (3) (may 2016)

A

a. only 10 % «of energy» can pass from one trophic level
to the next
b. energy released by respiration AND lost as heat (Not just respiration or heat.)
c. energy losses due to undigested parts/egestion
d. not enough energy for later stages of a food chain

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8
Q

(2c) Explain gene linkage and its effects on inheritance. [2] (Nov 2021)

A

a located on the same chromosome;
b genes/gene loci close together
c do not follow (the law of) independent assortment;
d more chance of recombination if genes are further apart;
e inherited together unless crossing over/recombination occurs;
f ratios of offspring in dihybrid crosses are different from expected/non-Mendelian
OR
more offspring with parental phenotype combinations than expected

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9
Q

(2d) Explain the mechanism that prevents polyspermy during fertilization. [2] (Nov 2021)

A

a cortical reaction (after first sperm nucleus enters the egg);
b vesicles/cortical granules release their contents/enzymes (from the egg/zygote);
c zona pellucida hardened
d enzymes of sperm/acrosome cannot digest (hardened coat)
OR
glycoproteins/ZP3 (in zona pellucida) altered so sperm cannot bind;

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10
Q

(3a) Outline what will happen to human red blood cells if transferred to distilled water. [1] (Nov 2021)

A

cells absorb water by osmosis and swell/increase in volume
OR
cells burst
(solution with high osmolarity: water leaves cell so cytoplasm shrinks in volume)

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11
Q

(3b) Stem cells can be used to treat Stargardt’s disease. State one other condition treated using stem cells. [1] (Nov 2021)

A

Leukemia

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12
Q

(3c) Explain the propagation of nerve impulses along the membrane of a neuron. [3] (Nov 2021)

A

a depolarization of part of axon causes depolarization of next part;
b local currents;
c diffusion of sodium ions between depolarized part and next/polarized part (of axon);
d resting potential reduced from -70 to -50mV;
e sodium channels open when -50mV threshold potential reached;
f entry of sodium ions causes depolarization;
g saltatory conduction in myelinated neurons/axons;

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13
Q

(4b) Describe the secondary structure of proteins. [2] (Nov 2021)

A

The formation of alpha helices and beta pleated sheets stabilized by hydrogen bonding

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14
Q
  1. (a) Compare and contrast the mode of nutrition of detritivores and saprotrophs. [2] (Nov 2021)
A

Similarity: heterotrophic/obtain nutrients from dead organic matter
Detritivores: internal digestion
Saprotrophs: external digestion

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15
Q

(5b) Explain how some plant species are able to respond to changes in their abiotic environment and flower at a precise time of the year. [3] (Nov 2021)

A

(Include gene expression, detection of light, specific time of year)
a genes for flowering are activated/gene activation/changes to gene expression;
b shoot apex changes from producing leaves/stem to producing flowers;
c daylength detected
d short day plants flower when they have a long period of darkness
OR
long day plants only flower when they have a short period of darkness;
e so short day plants flower in late summer/fall/autumn/winter
OR
so long day plants flower in spring/(early) summer;

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16
Q

(5c) Outline the extension of the stem in plants. [2] (Nov 2021)

A

a apical meristem (of shoot/stem) produces cells/elongates the stem
OR
cell division/mitosis in tip/apex of shoot/stem;
b auxin stimulates stem growth by…;
c elongation of cells

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17
Q

(8a) Outline the process of protein denaturation. [4] (Nov 2021)

A

a change to conformation/tertiary structure
b bonds within the protein/intramolecular bonds broken/changed;
c pH and temperature (outside tolerated ranges) can cause denaturation;
d heat at high temperatures breaks bonds;
e extreme pH alters ionization/charges (of amino acids and breaks ionic bonds);
f protein cannot carry out its function
OR
active site of enzymes cannot bind substrates/no enzyme-substate complex;
g irreversible change (usually)
OR
soluble proteins become insoluble/precipitate;

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18
Q

(8c) Distinguish between competitive and non-competitive enzyme inhibition. [4] (Nov 2021)

A

a binds to active site
binds to allosteric site

b inhibitor and substrate are (chemically) similar
inhibitor different from substrate;

c binding of substrate prevented (because active
site is occupied)
active site changed, preventing substrate binding;

d inhibition reduced by increasing substrate concentration
inhibition not affected by increased substrate concentration;

e useful as pharmaceuticals/toxins (competitive)
useful as end-product inhibitors;

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19
Q

(2b) Explain how the cell cycle is controlled. [4] (Nov 2016)

A

a. cell cycle is G1, S, G2 and mitosis

b. (control of the cell cycle) by cyclins
c. levels of cyclins fluctuate during the cell cycle
d. conditions inside and outside the cell affect regulation
e. four cyclins
cyclins regulate the timing of the cell cycle
f. cyclins bind to kinases and activate them
g. kinases phosphorylate other proteins
h. phosphorylated proteins perform specific functions in the cell cycle

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20
Q

(3b) Outline the importance of enzymes to metabolic processes. [4] (Nov 2016)

A

(mention how enzymes affect METABOLIC processes, not talking about action of enzymes)

a. increase rate of reaction
b. lower activation energy
c. a specific enzyme for each substrate
d. metabolic process blocked if an enzyme is inhibited/absent
e. end-product inhibition can control metabolic pathways
f. differences in metabolism as cells produce different enzymes during differentiation

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21
Q
  1. (a) List two causes of variation within a gene pool. [2] (Nov 2016)
A

a. sexual reproduction / random fertilization / meiosis (No mark for crossing over unqualified.
Reject natural selection/evolution as causes of variation.)

b. mutation

22
Q

(4b) Describe how variation contributes to evolution by natural selection. [3] (Nov 2016)

A

a. variation is different phenotypes (between individuals in a population)
b. competition for survival
c. some individuals have advantageous characteristics/are better adapted/have
greater chance of survival/reproduction (than others)
d. favourable alleles/genetic variations passed on/inherited by offspring/next generation

Reject “pass on phenotypes”.

23
Q

(c) Outline what is required for speciation to occur. [3] (Nov 2016) (topic 10.3)

A

a. divided species/gene pool/gene pool becomes separated
b. reproductive isolation
c. reproductive isolation may be due temporal, behavioral, geographic isolation
d. different selective pressures

24
Q

(7a) Describe the transport of organic compounds in vascular plants. [4] (Nov 2016)

A

a. phloem transports organic compounds/sucrose
b. from sources to sinks
c. through sieve tubes
d. loading of organic compounds by active transport/using ATP
e. high solute concentration causes water to enter by osmosis (at source)
f. high (hydrostatic) pressure causes flow (from source to sink)
g. companion cells help with loading
h. translocation/mass flow

25
Q

(7b) The flowers of angiospermophyta are used for sexual reproduction. Outline three processes required for successful reproduction of angiospermophyta. [3] (Nov 2016)

A

(Remembering production of gametes before pollination, pollination, fertilization and seed dispersal)

a. meiosis / production of male and female gametes
b. pollination / transfer of pollen from anther to stigma
c. fertilization happens after pollination
d. seed dispersal / spread of seeds to new locations 

26
Q

(8b) Producers extract phosphates and nitrates from soil. Outline how these ions are used
in the synthesis of organic molecules. [3] (Nov 2016)

A

(Mention process of synthesis, including the energy source, carbon compounds, what they can synthesize, how they are transported)

a. by photosynthesis / using energy from light
b. attached to carbon compounds
c. phosphates used to make phospholipids/nucleotides/nucleic acids/DNA/RNA/ATP
d. nitrates are used to make amino acids/proteins/nucleotides/nucleic
acids/DNA/RNA/ATP
e. transported from roots to leaves (in xylem)

27
Q

(8c) Describe how energy flows through and is used by organisms in ecosystems. [4] (Nov 2016)

A

(mention how different tropic levels obtain energy, what form is the energy in, how energy is used, ATP)

a. producers obtain energy from light/inorganic sources
b. energy passed in the form of carbon compounds (between trophic levels)
c. consumers obtain energy from previous trophic level
d. energy released by (cell) respiration (Reject energy used in respiration)
e. ATP produced
f. ATP used for biosynthesis/movement/active transport
g. energy lost at each trophic level

28
Q

(2b) Outline how depolarization of the membrane of an axon occurs. [2] (May 2021 TZ2)

A

a. (local depolarization) causes voltage gated channels to open (Do not accept sodium pumps)
b. Na+ diffuses into the cytoplasm
c. membrane potential (of the axon) changes from negative to positive
d. a threshold potential is reached and an action potential is generated

29
Q

(2c) Explain how acetylcholine initiates an action potential in a postsynaptic membrane. [2] (May 2021 TZ2)

A

a. acetylcholine binds to the receptor protein ✔
b. (causing a) change in tertiary structure of protein
c. diffusion of Na+ through the receptor protein

30
Q

(2d) (i) State the action of the enzyme acetylcholinesterase. [1] (May 2021 TZ2)

A

breaks down acetylcholine (to acetyl and choline) ✔

31
Q

2d (ii) Explain what happens to an enzyme if there is a change of pH. [3] (May 2021 TZ2)

A

a. change in pH can cause a change in the tertiary structure of the enzyme/enzyme denatured ✔
b. causing a change in the active site / substrate not able to bind ✔
c. decreased rate of reaction ✔
d. (may no longer be) the optimum/optimal pH for enzyme activity ✔

32
Q

(3b) An experiment was done to test the hypothesis that temperature affects the rate of
germination of the broad bean. Outline two factors apart from temperature that should
be controlled in this experiment. [2] (May 2021 TZ2)

A

(Germination doesn’t require light as seed is underground)

a. same amount/type of soil/ pH of soil ✔
b. same amount of water / humidity ✔
c. oxygen ✔
d. same measurement of germination / time ✔
e. same number/source/age of seeds ✔

33
Q

(3d) Broad beans are rich in starch and cellulose. Compare and contrast the structure of
starch and cellulose. [2] (May 2021 TZ2)

A

a. polysaccharides / both form 1,4 glycosidic bond ✔
b. starch is formed by alpha glucose while cellulose is formed by beta glucose
OR
in starch C1 hydroxyl groups are found in same plane while in cellulose on different planes
OR
in cellulose, alternatively the beta glucose needs to be placed upside-down in order to have C1 hydroxyl groups on the same plane
OR
two types of starch (amylose and amylopectin) but one type of cellulose ✔

34
Q

(4a) State one process that results in the loss of carbon dioxide from a marine organism
such as a crustacean or a jellyfish. [1] (May 2021 TZ2)

A

a. (aerobic/cellular) respiration ✔

b. gas exchange / diffusion ✔

35
Q

(4b) The crustacean and the jellyfish obtain carbon compounds by feeding. State one
source of carbon for marine organisms, other than feeding. [1] (May 2021 TZ2)

A

a. photosynthesis ✔

b. absorption of (dissolved) carbon dioxide

36
Q

(4c) Explain how energy enters, flows through and is lost from marine food chains. [3] (May 2021 TZ2)

A

a. light energy is converted to chemical energy (in carbon compounds/sugars) by photosynthesis ✔
b. (chemical) energy (in carbon compounds) flows by means of feeding/through food chains/webs ✔
c. only (approximately) 10% of energy is passed to the next trophic level ✔
d. energy released as heat (by respiration) ✔
e. energy is not recycled ✔
f. after death, energy may remain trapped as fossils

37
Q

(5b) Outline how sperm are produced from diploid cells in the testis and how this production can be sustained over many decades of adult life. [4] (May 2021 TZ2)

A

(Detail description of spermatogenesis)

a. germinal epithelium divide endlessly (by mitosis giving rise to spermatagonia)
b. spermatogonia are diploid/2n
c. spermatogonia divide by mitosis
d. spermatogonia enlarge forming primary spermatocytes ✔
e. primary spermatocytes undergo meiosis I
f. secondary spermatocytes produced are haploid/n ✔
g. secondary spermatocytes undergo meiosis 2(to produce spermatids) ✔
h. spermatids develop tails/spermatids differentiate into spermatozoa / spermatids associate with Sertoli cells ✔

38
Q

(5c) Testis cells are eukaryotic cells. Identify the structures seen under the electron
microscope in testis cells that are not present in prokaryotic cells. [4] (May 2021 TZ2)

A

a. nucleus/nuclear membrane ✔
b. membrane bound organelles ✔
c. mitochondria ✔
d. rough ER/smooth ER/golgi apparatus ✔
e. lysosomes / centrioles ✔
f. 80S ribosomes
g. linear chromosomes / histones ✔

39
Q

(6b) Describe how the structure of the chloroplast is adapted to its function in photosynthesis. [4] (May 2021 TZ2)

A

a. thylakoids have a small internal volume to maximize hydrogen gradient
b. many grana give large surface area ✔
c. (grana) contain (photosynthetic) pigments/electron carriers/ATP synthase enzymes ✔
d. (photosynthetic) pigments arranged into photosystems allowing maximum absorption of light energy ✔
e. stroma has suitable pH/ enzymes of Calvin cycle ✔
f. lamellae connect/separate grana maximizing photosynthetic efficiency ✔

40
Q

(6c) The enzyme Rubisco is used in carbon fixation during photosynthesis. Identify four
other examples of proteins that illustrate the wide range of functions of this group of
biochemicals in living organisms. [4] (May 2021 TZ2)

A

a. insulin receptors start the cellular signalling pathway/other receptor
b. leptin is a hormone / other protein hormone ✔
c. immunoglobulins/antibodies defend body from disease ✔
d. photosystems convert light energy to electrical impulses ✔
e. keratin/collagen is a structural protein ✔
f. microtubules part of cytoskeleton/ involved in cell division
g. ATP synthase is an enzyme that catalyzes the formation of ATP / other enzyme and function ✔
h. fibrin/fibrinogen is a protein involved in clotting ✔
i. hemoglobin/sodium potassium pump/other example is a transport protein ✔
j. actin/myosin is involved in muscle contraction ✔
m. spider silk is used to form spider webs

41
Q
  1. (a) Describe the structure of the DNA molecule. [5] (May 2021 TZ2)
A

(Do not mention nucleosomes, histones as that would be chromosome structure)
(Drawing diagram is recommended)

a. two stranded/double helix ✔
b. antiparallel / one strand organized 5’ to 3’ and the other 3’ to 5’ ✔
c. sugar-phosphate backbone ✔
d. each strand formed by chains of nucleotides ✔
e. each nucleotide is formed by a phosphate, a deoxyribose and a base / annotated diagram
f. the bases are adenine, guanine, cytosine and thymine
g. strands held together by hydrogen bonds (between complementary base pairs)

42
Q

(b) Outline the role of three enzymes used in the replication of DNA. [3] (May 2021 TZ2)

A

(Always write more points than the marks)

a. helicase unwinds DNA strands ✔
b. gyrase relax the tension as bacterial DNA is being uncoiled / prevent supercoiling ✔
c. primase to synthesise primers ✔
d. polymerase (I) removes primers and replaces with nucleotide ✔
e. polymerase (III) adds nucleotides (in a 5’ to 3’ direction) ✔
f. ligase joins (Okazaki) fragments together ✔

43
Q

Outline the roles of estrogen and progesterone in females during human reproduction.

A

Estrogen and progesterone inhibit the secretion of LH and FSH via negative feedback.
Progesterone maintains the endometrium, allowing the implantation of blastocyst
progesterone for development of breast tissue
fall in progesterone/rise in estrogen leads to labour
Cause sexual secondary characteristics

44
Q

Outline the reasons for the differences in blood concentrations between the renal artery and the renal vein. (4)

A

urea lower in vein due to excretion/ultrafiltration
Oxygen lower in vein due to cell respiration
glucose lower in vein due to cell respiration
solute concentration lower in vein if ADH secreted

45
Q

Discuss whether skeletal muscle consists of cells or not. (2)

A

multinucleate. Cells are expected to have one nucleus so muscle cells are an exception to the cell theory.
One cell membrane enclosing a whole muscle fibre
Larger than most cells.

46
Q

(2b) Outline the causes of sickle cell anemia. [2] (May 2019 TZ1)

A

a. genetic disease
b. base substitution mutation/GAG → GTG ✔
c. hemoglobin gene mutated/HbA → HbS ✔
d. leads to change in amino acid sequence «in hemoglobin»/glutamic acid → valine ✔
e. only homozygotes have full sickled cells
f. gives resistance to malaria ✔

47
Q

(4b) Explain how the properties of water allow it to move through xylem vessels. [2] (May 2019 TZ1)

A

a. water is polar
b. polarity results in hydrogen bonds
c. hydrogen bonding causes cohesion of water
d. cohesion allows water to withstand tension/withstand low pressure
e. cohesion/hydrogen bonding prevents column of water «in xylem» from breaking
f. adhesion of water to xylem walls «due to hydrogen bonds» ✔

48
Q

(4c) Outline how the structure of cellulose makes it suitable as a component of cell walls. [2] (May 2019 TZ1)

A

a. 1-4 glycosidic linkages
b. beta glucose forms straight chains/alternating orientation of glucose units
c. forms microfibrils
d. high tensile strength

49
Q

(5b) Outline the control of metabolism by end-product inhibition. [5] (May 2019 TZ1)

A

a. metabolism is chains/web of enzyme-catalyzed reactions
b. end product/inhibitor is final product of pathway ✔
c. inhibits the first enzyme in pathway ✔
d. non-competitive inhibition ✔
e. end-product binds to an allosteric site
f. changes the shape of the active site
g. prevents intermediates from building up
h. negative feedback ✔
i. inhibitor is reversible
j. isoleucine inhibits/slows «activity of first enzyme in» threonine to isoleucine pathway ✔

50
Q
  1. (a) Outline the process of inspiration in humans. [4] (May 2019 TZ1)
A

(Contraction of muscles –> action: increase thorax volume –> pressure decreases)

a. diaphragm and external intercostal muscles contract
b. diaphragm moves down/becomes flatter
OR
external intercostals raise the ribcage
c. diaphragm increase volume of thorax
d. as volume «of thorax» increases the pressure decreases ✔
e. air enters «lungs» due to higher pressure outside body ✔
f. air flows to lungs through trachea and bronchi/bronchioles ✔

51
Q

(6b) Describe the functions of valves in the mammalian heart. [4] (May 2019 TZ1)

A

a. prevents backflow
b. open valves allow blood to flow through
c. closed «semilunar» valves allow ventricles/chambers to fill with blood / allow pressure in ventricles to rise «rapidly» ✔
d. valves open when pressure is higher upstream
e. AV valves prevent backflow from ventricle to atrium
f. semilunar valves prevent backflow from artery to ventricle