2. Molecular Biology Flashcards

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1
Q

Explain the production of lactose-free milk.

A

Filtered through immobilised lactase
hydrolyse lactose to glucose and galactose
For lactose intolerant people

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2
Q

Distinguish between the structure of the chromosomes of prokaryotes and eukaryotes. (2)

A

Prokaryotes have circular DNA but eukaryotes have linear chromosomes
Histones associated with DNA in eukaryotes but naked DNA in prokaryotes
Some prokaryotes have plasmids but eukaryotes don’t
Eukaryotes have multiple chromosomes whereas prokaryotes typically have one
Eukaryotes have telomeres but prokaryotes don’t
Eukaryotes DNA can supercoil due to histones but not prokaryote DNA

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3
Q

Explain the secondary structure of alpha amylase.

A

alpha helices, beta pleated sheets
The picture mainly consists of alpha helices
Some beta pleated sheets in the picture
spiral coils held together by hydrogen bonds
hydrogen bonds between oxygen C-O and hydrogen atoms N-H of amino acids

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4
Q

Explain how enzymes catalyse chemical reactions

A
Enzymes form enzyme-substrate complexes
binding of substrate to active site of enzyme
enzyme changes shape slightly
decreases activation energy
Enzymes bind to specific substrates
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5
Q

State the function of amylase and the site of production.

A

breakdown starch to maltose. Salivary glands and pancreas

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6
Q

What is maltase break down? Where does digestion of starch mostly take place?

A

Maltose into glucose. Small intestines.

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7
Q

What does lactase break down?

A

lactose into glucose and galactose.

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8
Q

What does sucrase break down?

A

sucrose into glucose and fructose.

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9
Q

Explain, with reference to the thermal properties of water, how humans respond to overheating.

A

Water has high latent heat of vaporization
More energy required to evaporate water (breaking hydrogen bonds)
Evaporation has cooling effect, water is a good evaporative coolant
sweating as an example of using water as a coolant

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