sft CSF Cytology Flashcards
CSF adequacy criteria
none, no cells required
CSF, cell type? Staining?
Benign ependymal cells, more common in VP shunt; bland, spindled to epithelioid cells; EMA+ and CD99+
CSF, cell type? Staining?
Choroid plexus cells, cobblestone like cluster of epitheliod cells that are CK+; have intracytoplasmic vacuoles of varying sizes
CSF, diagnosis?
Benign marrow elements
CSF cytology, dx?
Benign brain parenchyma (can see in VP shunt or occasionally trauma); fluffy material (neuropil) and vessels with glial cells
What are the most common organisms implicated in acute bacterial meningitis in immunocompetenet adults?
Streptococcus pneumoniae and Neisseria meningitidis
Causes of acute fungal meningitis in the immunocompromised?
Cryptococcus neoformans (narrow based budding), Candida albicans, and Aspergillus fumigatus
Cytologic clue to HSV meningoencephalitis, most useful method for definite diagnosis.
Hemorrhage in CSF (red cells, hemosiderin-laden macrophages); PCR is used for viral detection
CSF
Cryptococcus (narrow based budding)
CSF
Candida
CSF, possible diagnosis?
HSV meningoencephalitis; increased lymphocytes and lipid-laden macrophages
What is the most common pathogen isolated from Guillian Barre patients?
Campylobacter jejuni
CSF; 1st in differential with these cells? What would you see on electropheresis?
Multiple sclerosis (plasma cells and lymphocytes); would see oligoclonal IG bands
CSF, dx?
•Glioblastoma: Medium to large , loosely cohesive tumor cells with hyperchromatic nuclei and a variable amount of cytoplasm; dirty background
CSF, dx?
Medulloblastoma: Malignant small blue cells appear singly or arranged in small cohesive groups; high N/C ratio, mitotic figures
CSF, dx?
Choroid plexus carcinoma: cohesive cluster of large malignant cells with markedly irregular nuclear membranes/vacuoles.
CSF, dx?
CLL, atypical lymphocytes with irregular nuclei
