Serum Bilirubin

Question 0

Wavelength

Answer 0

546 nm

Question 1

Product is a red azo dye

Answer 1

Reaction of bilirubin with diazotised sulphanilic acid (DSA)

Question 2

It is directly proportional to the bilirubin concentration of the sample

Answer 2

Absorbance of red azo dye

Question 3

Water soluble bilirubin glucuronides:

Albumin conjugated indirect bilirubin:

Answer 3

DSA

DSA in the presence of accelerator (total bilirubin = direct + indirect bilirubin)

Question 4

Stability

Answer 4

Expiry date if unopened and stored at 15-18 Celsius

Question 5

May reduce bilirubin levels

Answer 5

Exposure to light

Hemolysis (inhibitory effect of hemoglobin on the diazo reaction)

Question 6

Reference range

Answer 6

(Review)

Question 7

Temperature

Answer 7

20-25 Celsius

Question 8

Optical path

Answer 8

1 cm

Question 9

Total bilirubin

Answer 9

Incubate at room temperature for 10 to 30 minutes

Question 10

Direct bilirubin

Answer 10

Mix, incubate at room temperature for exactly 5 minutes

Question 11

Calculation

Answer 11

Bilirubin concentration [mg/dL] = absorbance at 546 nm x 13.0
[mg/dL] 17.1 = [mmol/L]

Question 12

Sulphanilic acid + sodium nitrite —>

Answer 12

DSA

Question 13

_____ —> DIRECT Azobilirubin

Answer 13

Bilirubin + DSA

Question 14

Bilirubin + DSA + accelerator —>

Answer 14

TOTAL Azobilirubin

Question 15

Contents

Answer 15

1 Total bilirubin reagent (white cap)
2 T-Nitrite reagent (white cap)
3 Direct bilirubin reagent (red cap)
4 D-Nitrite reagent (red cap)