Hemoglobin Measurement Flashcards
0
Q
Precautions in hemoglobin measurement
A
- Salts and solution of cyanide are poisonous. Care should be taken while handling such salts and solution.
- Drabkin’s solution is sensitive to light and should be stored in a brown bottle and in the dark.
- Drabkin’s solution contains cyanide. When filling pipettes, a suction bulb should be used.
- Drabkin’s solution should not be used after precipitate has formed in the bottom of the storage bottle.
1
Q
Other term for Drabkin’s reagent
A
Potassium ferricyanide-cyanide solution
2
Q
It is measured photometrically.
A
Intensity of cyanmethemoglobin
3
Q
Other term for hemoglobin measurement
A
Cyanmethemoglobin method
4
Q
Formula for hemoglobin concentration
A
Hb (g/dL) = 36.8 x Abs
5
Q
Action of Drabkin’s solution
A
Oxidizes hemoglobin to methemoglobin which is in turn converted to cyanmethemoglobin
6
Q
Set wavelength at ______
A
540 nm
7
Q
Allow the tubes to stand for _______
A
10 minutes
8
Q
Components of the reagent
A
1 g of sodium bicarbonate
- 52 g of potassium cyanide
- 198 g of potassium ferricyanide
9
Q
Sample used for hemoglobin measurements
A
Whole blood
11
Q
Hemoglobin normal range
A
Female 12-16 g/dL
Male 14-18 g/dL
