Aspartate Aminotransferase

Question 0

Reaction principle

Answer 0

1 2-oxoglutarate + L-aspartate – (GOT) – L-glutamate + oxaloacetate
2 Oxaloacetate + NADH + H – (MDH) – L-malate + NAD

Question 1

Method for Aspartate Aminotransferase activity determination

Answer 1

Kinetic method (Expert Panel of the International Federation of Clinical Chemistry)

Question 2

Contents

Answer 2

Reagent 1/Enzyme reagent

Reagent 2/Starting reagent

Question 3

Stability of reagent start

Answer 3

The reagents are stable even after opening up to the stated expiry date when protected from light at 2-8 Celsius.

Question 4

Stability of sample start

Answer 4

The working reagent is stable for 4 weeks at 2-8 Celsius and 5 days at 15-25 Celsius.

Question 5

Specimen

Answer 5

1 Serum
2 Heparinized plasma
3 EDTA plasma

Question 6

Loss of activity

Answer 6

1 Within 3 days at 4 Celsius: 8%

2 At 20-25 Celsius: 10%

Question 7

Reference range

Answer 7

(Review)

Question 8

Conversion factor

Answer 8

(Review)

Question 9

Contain sodium azide

Answer 9

Enzyme reagent and starting reagent

Question 10

Optical path

Answer 10

1 cm

Question 11

Wavelength

Answer 11

Hg 365 nm, 340 nm or Hg 334

Question 12

Temperature

Answer 12

1 25 Celsius
2 30 Celsius
3 37 Celsius

Question 13

Measurement

Answer 13

Against air (decreasing absorbance)

Question 14

Procedure

Answer 14

Mix, read the absorbance after 1 minute and at the same time start the stop watch. Read the absorbance again exactly after 1, 2, and 3 minutes.

Question 15

Calculation measurements

Answer 15

For absorbance changes per minute within 0.06-0.08 (Hg 36 nm) or 0.12-0.16 (Hg 334 nm, 340 nm) (procedure 1+2) only measurements from the first 2 minutes are used for calculation (1 minute incubation, 2 minutes measurement).