Serology Micro Flashcards
Provides history of pt
Serology
Used to identify the infecting agent
Serology
Used to identify viruses and other agents that is difficult to isolate and grow in lab or slowly progress
Serologic testing
Virus diagnosed by serology
Ebv
Rubella
Hep ABCDE
HIV
HUMAN T CELL LEUKEMIA VIRUS
ARBO V ( encephalitis)
Used to determine persons immune status
Serologic testing
Greatest dilution of sample that retains a detectable activity
Titer
Relative AB CONCENTRATIONS
Tites
Inverse of the greatest dilution ( lowest con)
Titer
Evaluate the amount out IG MGIE reactive with antigen
Labeled second antihumanantibody
Specific for the antibody isotope
Labeled second antihumanantibody
Occurs when AB is produces in response to PRIMARY INFection’
Seroconversion
Least fourfold increase in the AB
Seroconversion
Specific igM AB Found during at least 2-3 weeks later during convalescent phase
Seroconversion
Caused by re infection or recurrence later in life
Anamnestic
Herpesvirus
Anamnestic
Detect, identify and quantity antigen in clinical sample
Immunologic techniques
Detect, identify and quantity antigen in clinical sample
Immunologic techniques
Evaluate the antibody response to infection and persons history of exposure to infectious agents
Immunologic techniques
Used as sensitive and specific tools to detect, identify and quantitate solute antigen and antigens in cell from a virus, bacterium, fungus or parasite
Antibody
Cheap to produce
Polyclonal
Mixed population of AB
Polyclonal
Heterozygous AB that can recognize many epitopes on a single antigen
Polyclonal
Bind to diff areas of target molecule
Polyclonal
Tolerant of small changes in protein structure
Polyclonal
Expensive to produce
Single AB
Monoclonal
Recognizes individual epitope on an antigen
Monoclonal
Bind to specific site and recognize particular protein form
Monoclonal
Prefer to be industrial sized tissue culture preparation
Monoclonal
A limited conc range for both antigen and AB
Equivalence zone
Where the precipitation of protein occurs
Equivalence zone
Detect and quantify an antigen
Single radial immunodiffusion
Antigen is placed into well and allowed to diffuse into AB containing agar
SINGLE RADIAL IMMUNODIFFUSION
Preicipitin rings indicate an immune rxn and the area of the ring is proportional to the conc of antigen
Single radial immunodi
The higher the con of antigen , the farther it diffuse before it reaches equivalent with the AB in the agar and ppt as ring around the well
SRI
Used to determine relatedness of different antigens
Ouchterlony immuno double diffusion
Used to detect antibody and fungal antigens
Ouchterlony immuno double diff
Hsitoplasma spcs
Balstomyces spcs
Coccidioidomycoses
Ouchterlony immuno double diff
In this technique solutions of antibody and antigen are placed into well separate well cut into agar
Ouchterlony
Establish ring of concentration gradients if each substance
Ouchterlony
A visible precipitin line occurs where the conc of antigen and antibody reach equivalence
Ouchterlony
Identity
Non identity
Partial identity
Ouchterlony
Antigen are placed in adjacent wells then the conc of antigen bet them is doubled and ppt does not occur
Identical or identity
Antigen are placed in adjacent wells then the conc of antigen bet them is doubled and ppt does not occur
Identical or identity
Antigen are used two different precipitin line produced
Non identity
One sample shares antigen but is not identical, then a single spur results for the complete antigen
Partial identity
Antigen is placed into well a well and separated by electrophoresis
Immunoelectrophoresis
Antibody is then placed in the trough and precipitin lines form as antigen and antibody diffuse toward each other
Immunoeletrophoresis
Antigens are separated by electrophores into an agar gel that contains antibody
Rocket electrophoresis
The length of the rocket indicates conc of antigen
Rocket electrophoresis
Antigen and AB may be placed in separate wells and allowed to move eltrophoretically tod
Ward each other
Countercurrent immunoeletrophoresis
Similar to Ouchterlony method but the antigen movement is facilitated by elerophoresis
Countercurrent immunoelectrophoresis
