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Serology Micro Flashcards

1
Q

Provides history of pt

A

Serology

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2
Q

Used to identify the infecting agent

A

Serology

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3
Q

Used to identify viruses and other agents that is difficult to isolate and grow in lab or slowly progress

A

Serologic testing

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4
Q

Virus diagnosed by serology

A

Ebv
Rubella
Hep ABCDE
HIV
HUMAN T CELL LEUKEMIA VIRUS
ARBO V ( encephalitis)

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5
Q

Used to determine persons immune status

A

Serologic testing

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6
Q

Greatest dilution of sample that retains a detectable activity

A

Titer

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7
Q

Relative AB CONCENTRATIONS

A

Tites

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8
Q

Inverse of the greatest dilution ( lowest con)

A

Titer

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9
Q

Evaluate the amount out IG MGIE reactive with antigen

A

Labeled second antihumanantibody

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10
Q

Specific for the antibody isotope

A

Labeled second antihumanantibody

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11
Q

Occurs when AB is produces in response to PRIMARY INFection’

A

Seroconversion

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12
Q

Least fourfold increase in the AB

A

Seroconversion

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13
Q

Specific igM AB Found during at least 2-3 weeks later during convalescent phase

A

Seroconversion

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14
Q

Caused by re infection or recurrence later in life

A

Anamnestic

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15
Q

Herpesvirus

A

Anamnestic

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16
Q

Detect, identify and quantity antigen in clinical sample

A

Immunologic techniques

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17
Q

Detect, identify and quantity antigen in clinical sample

A

Immunologic techniques

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18
Q

Evaluate the antibody response to infection and persons history of exposure to infectious agents

A

Immunologic techniques

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19
Q

Used as sensitive and specific tools to detect, identify and quantitate solute antigen and antigens in cell from a virus, bacterium, fungus or parasite

A

Antibody

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20
Q

Cheap to produce

A

Polyclonal

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21
Q

Mixed population of AB

A

Polyclonal

22
Q

Heterozygous AB that can recognize many epitopes on a single antigen

A

Polyclonal

23
Q

Bind to diff areas of target molecule

A

Polyclonal

24
Q

Tolerant of small changes in protein structure

A

Polyclonal

25
Expensive to produce Single AB
Monoclonal
26
Recognizes individual epitope on an antigen
Monoclonal
27
Bind to specific site and recognize particular protein form
Monoclonal
28
Prefer to be industrial sized tissue culture preparation
Monoclonal
29
A limited conc range for both antigen and AB
Equivalence zone
30
Where the precipitation of protein occurs
Equivalence zone
31
Detect and quantify an antigen
Single radial immunodiffusion
32
Antigen is placed into well and allowed to diffuse into AB containing agar
SINGLE RADIAL IMMUNODIFFUSION
33
Preicipitin rings indicate an immune rxn and the area of the ring is proportional to the conc of antigen
Single radial immunodi
34
The higher the con of antigen , the farther it diffuse before it reaches equivalent with the AB in the agar and ppt as ring around the well
SRI
35
Used to determine relatedness of different antigens
Ouchterlony immuno double diffusion
36
Used to detect antibody and fungal antigens
Ouchterlony immuno double diff
37
Hsitoplasma spcs Balstomyces spcs Coccidioidomycoses
Ouchterlony immuno double diff
38
In this technique solutions of antibody and antigen are placed into well separate well cut into agar
Ouchterlony
39
Establish ring of concentration gradients if each substance
Ouchterlony
40
A visible precipitin line occurs where the conc of antigen and antibody reach equivalence
Ouchterlony
41
Identity Non identity Partial identity
Ouchterlony
42
Antigen are placed in adjacent wells then the conc of antigen bet them is doubled and ppt does not occur
Identical or identity
43
Antigen are placed in adjacent wells then the conc of antigen bet them is doubled and ppt does not occur
Identical or identity
44
Antigen are used two different precipitin line produced
Non identity
45
One sample shares antigen but is not identical, then a single spur results for the complete antigen
Partial identity
46
Antigen is placed into well a well and separated by electrophoresis
Immunoelectrophoresis
47
Antibody is then placed in the trough and precipitin lines form as antigen and antibody diffuse toward each other
Immunoeletrophoresis
48
Antigens are separated by electrophores into an agar gel that contains antibody
Rocket electrophoresis
49
The length of the rocket indicates conc of antigen
Rocket electrophoresis
50
Antigen and AB may be placed in separate wells and allowed to move eltrophoretically tod Ward each other
Countercurrent immunoeletrophoresis
51
Similar to Ouchterlony method but the antigen movement is facilitated by elerophoresis
Countercurrent immunoelectrophoresis

Heart Failure (3 decks)

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