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Heart Failure > Serology Micro > Flashcards

Serology Micro Flashcards

1
Q

Provides history of pt

A

Serology

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2
Q

Used to identify the infecting agent

A

Serology

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3
Q

Used to identify viruses and other agents that is difficult to isolate and grow in lab or slowly progress

A

Serologic testing

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4
Q

Virus diagnosed by serology

A

Ebv
Rubella
Hep ABCDE
HIV
HUMAN T CELL LEUKEMIA VIRUS
ARBO V ( encephalitis)

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5
Q

Used to determine persons immune status

A

Serologic testing

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6
Q

Greatest dilution of sample that retains a detectable activity

A

Titer

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7
Q

Relative AB CONCENTRATIONS

A

Tites

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8
Q

Inverse of the greatest dilution ( lowest con)

A

Titer

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9
Q

Evaluate the amount out IG MGIE reactive with antigen

A

Labeled second antihumanantibody

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10
Q

Specific for the antibody isotope

A

Labeled second antihumanantibody

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11
Q

Occurs when AB is produces in response to PRIMARY INFection’

A

Seroconversion

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12
Q

Least fourfold increase in the AB

A

Seroconversion

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13
Q

Specific igM AB Found during at least 2-3 weeks later during convalescent phase

A

Seroconversion

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14
Q

Caused by re infection or recurrence later in life

A

Anamnestic

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15
Q

Herpesvirus

A

Anamnestic

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16
Q

Detect, identify and quantity antigen in clinical sample

A

Immunologic techniques

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17
Q

Detect, identify and quantity antigen in clinical sample

A

Immunologic techniques

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18
Q

Evaluate the antibody response to infection and persons history of exposure to infectious agents

A

Immunologic techniques

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19
Q

Used as sensitive and specific tools to detect, identify and quantitate solute antigen and antigens in cell from a virus, bacterium, fungus or parasite

A

Antibody

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20
Q

Cheap to produce

A

Polyclonal

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21
Q

Mixed population of AB

A

Polyclonal

22
Q

Heterozygous AB that can recognize many epitopes on a single antigen

A

Polyclonal

23
Q

Bind to diff areas of target molecule

A

Polyclonal

24
Q

Tolerant of small changes in protein structure

A

Polyclonal

25
Q

Expensive to produce
Single AB

A

Monoclonal

26
Q

Recognizes individual epitope on an antigen

A

Monoclonal

27
Q

Bind to specific site and recognize particular protein form

A

Monoclonal

28
Q

Prefer to be industrial sized tissue culture preparation

A

Monoclonal

29
Q

A limited conc range for both antigen and AB

A

Equivalence zone

30
Q

Where the precipitation of protein occurs

A

Equivalence zone

31
Q

Detect and quantify an antigen

A

Single radial immunodiffusion

32
Q

Antigen is placed into well and allowed to diffuse into AB containing agar

A

SINGLE RADIAL IMMUNODIFFUSION

33
Q

Preicipitin rings indicate an immune rxn and the area of the ring is proportional to the conc of antigen

A

Single radial immunodi

34
Q

The higher the con of antigen , the farther it diffuse before it reaches equivalent with the AB in the agar and ppt as ring around the well

A

SRI

35
Q

Used to determine relatedness of different antigens

A

Ouchterlony immuno double diffusion

36
Q

Used to detect antibody and fungal antigens

A

Ouchterlony immuno double diff

37
Q

Hsitoplasma spcs
Balstomyces spcs
Coccidioidomycoses

A

Ouchterlony immuno double diff

38
Q

In this technique solutions of antibody and antigen are placed into well separate well cut into agar

A

Ouchterlony

39
Q

Establish ring of concentration gradients if each substance

A

Ouchterlony

40
Q

A visible precipitin line occurs where the conc of antigen and antibody reach equivalence

A

Ouchterlony

41
Q

Identity
Non identity
Partial identity

A

Ouchterlony

42
Q

Antigen are placed in adjacent wells then the conc of antigen bet them is doubled and ppt does not occur

A

Identical or identity

43
Q

Antigen are placed in adjacent wells then the conc of antigen bet them is doubled and ppt does not occur

A

Identical or identity

44
Q

Antigen are used two different precipitin line produced

A

Non identity

45
Q

One sample shares antigen but is not identical, then a single spur results for the complete antigen

A

Partial identity

46
Q

Antigen is placed into well a well and separated by electrophoresis

A

Immunoelectrophoresis

47
Q

Antibody is then placed in the trough and precipitin lines form as antigen and antibody diffuse toward each other

A

Immunoeletrophoresis

48
Q

Antigens are separated by electrophores into an agar gel that contains antibody

A

Rocket electrophoresis

49
Q

The length of the rocket indicates conc of antigen

A

Rocket electrophoresis

50
Q

Antigen and AB may be placed in separate wells and allowed to move eltrophoretically tod
Ward each other

A

Countercurrent immunoeletrophoresis

51
Q

Similar to Ouchterlony method but the antigen movement is facilitated by elerophoresis

A

Countercurrent immunoelectrophoresis

Heart Failure (3 decks)

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