Serology

Question 1

Normal saline solution isotonic concentration

Answer 1

0.85% NaCl

Question 2

Alkaline phosphatase color and source

Answer 2

Yellow (E. coli)

Question 3

Peroxidase color and source

Answer 3

Green (horseradish)

Question 4

Fluorescence color of FITC

Answer 4

Green fluorescence

Question 5

Half life of 125 ^ I

Answer 5

60 days

Question 6

RIA that can be used to measure total IgE (competitive)

Answer 6

Radioimmunosorbent test (RIST)

Question 7

RIA used to measure allergen specific IgE (noncompetitive)

Answer 7

Radioallergosorbent test (RAST)

Question 8

RIA noncompetitive (sandwich assay)

Answer 8

Immunoradiometric Assay (IRMA)

Question 9

Structure of DNA

Answer 9

Helical, double-stranded

Question 10

Purine bases

Answer 10

Adenine, Guanine

Question 11

Pyrimidine bases (DNA)

Answer 11

Cytosine and Thymine

Question 12

Cofactor to polymerase

Answer 12

MgCl2 (Mg)

Question 13

3 steps of PCR

Answer 13

1. Denaturation (95 deg C)
2. Annealing (52 deg C)
3. Extension or Elongation or Synthesis (72 deg C)

Question 14

Molecular Blotting Techniques
Southern blot -
Northern Blot - 
Western Blot -
Eastern Blot -

Answer 14

SB - DNA
NB - RNA
WB - protein and glycoprotein
EB - lipids and glycolipids

Question 15

Source of complement for complement related testing

Answer 15

Guinea pig

Question 16

Indicator cell of complement related tests

Answer 16

Sensitized sheep RBCs

Question 17

First diagnostic blood test for syphilis

Answer 17

Wasserman test

Question 18

Interpretation of results for TPI

Answer 18

Positive > 50% immobilized
Negative <20% immobilized
Doubtful 20-50% immobilized

Question 19

Rbc control for neutralization/inhibition should show

Answer 19

No hemolysis

Question 20

SLO control for neutralization test should show

Answer 20

Hemolysis

Question 21

Most common source of alkaline phosphatase in immunoassays

Answer 21

Calf intestine

Question 22

Natural clumping / fleecy mass when a suspension of antigens and antibodies is agitated

Answer 22

Flocculation

Question 23

Net flow of hydrated ions in one direction when the electric field is applied

Answer 23

Endo-osmosis or electro-osmosis

Question 24

Which complement has the lowest concentration of all complement proteins

Answer 24

Factor D

Question 25

Main function is to bind to C8 and prevent insertion of C9 into host cell membranes

Answer 25

MIRL (CD59)

Question 26

Another name for CD 21 (receptor for Epstein Barr Virus)

Answer 26

CR2

Question 27

Another name for CR3 (adhesion and increased activity of phagocytic cells

Answer 27

CD11b/CD18

Question 28

Represents the classes of immunoglobulins

Answer 28

Isotype

Question 29

Direct antiglobulin test can detect in vivo sensitization of red cells by (?)

Answer 29

IgG or C3d

Question 30

Serological test used to determine the presence of immunity to Corynebacterium diphtheriae

Answer 30

Shick’s test

Question 31

Serological test used to identify susceptibility to scarlet fever

Answer 31

Dick’s test

Question 32

Antigen associated with acetylcholinesterase

Answer 32

Cartwright

Question 33

What is used in the assay for AH50 test for the overall deficiencies of the alternative parhway

Answer 33

Rabbit

Question 34

Proponent for phagocytosis

Answer 34

Elie Metchnikoff

Question 35

Proponent for complement

Answer 35

Jules Bordet

Question 36

Proponent for monoclonal Ab

Answer 36

George Kohler | And Cesar Milstein

Question 37

Proponent for Ab diversity

Answer 37

Susumo Tonegawa

Question 38

Inadequate washing in enzyme immunoassays

Answer 38

Causes false positive results

Question 39

Which is more sensitive (homogenous or heterogeneous assays)

Answer 39

Heterogenous is more sensitive than homogeneous

Question 40

Fluorescence is the property of some compounds to absorb light of one wavelength and emit light of _____

Answer 40

Longer wavelength

Question 41

What antigen serves as the receptor for the attachment of the peptic-ulcer causing bacteria (H. pylori) to the gastric mucosa

Answer 41

Lewis antigen

Question 42

Responsible for the slapped cheek appearance of parvovirus B19

Answer 42

P blood group system

Question 43

Known as CALLA (common ALL antigen)

Answer 43

CD10

Question 44

Known as Leukocyte Common Antigen (LCA)

Answer 44

CD45

Question 45

Most common phenotype in the black population for Rh

Answer 45

Dce

Question 46

Forssman antibodies (?) guinea pig

Answer 46

Forssman antibodies absorbed by guinea pig

Question 47

Frequency of the Kell phenotype

Answer 47

K-k+ > K+k+ > K+ k-

Question 48

Form of immune complex type of hypersensitivity reaction that results from build up of antibodies to the animal serum used in some passive immunization

Answer 48

Serum sickness

Question 49

Minimum number of WBC count in a leukopoor platelet preparation

Answer 49

<8.3 x 10^5

Question 50

Minimum wbc count of pooled platelets

Answer 50

5.0 x 10^6

Question 51

(+) DAT (-) antibody screen Is because of

Answer 51

Complement coating rbcs | Drug dependent ab coating red cells

Question 52

Most warm autoantibodies are directed against

Answer 52

Rh system

Question 53

Most common type or transfusion reaction

Answer 53

Febrile nonhemolytic transfusion reaction

Question 54

Ab of which blood group is most often associated with an anamnestic response

Answer 54

ABO

Question 55

Records of transfusion reactions should be kept ___

Answer 55

Indefinitely

Question 56

Examples of reverse passive agglutination

Answer 56

CRP | RF

Question 57

IgM that directed fo the Fc portion of the IgG

Answer 57

Rhematoid Factor

Question 58

Post thaw storage of cryoprecipitate

Answer 58

Room temperature, transfuses within 6 hrs | If pooled - 4 hrs

Question 59

Minimum number of WBC count in leukopoor RBC pack

Answer 59

<5.0 x10^6

Question 60

Confirmation of heterophile antibody for infectious mononucleosis would be:

Answer 60

Agglutination of sheep cells after incubation with guinea pig cells No agglutination of sheep cells after incubation with beef cells

Question 61

Ab to which Ag is first detected in HIV infection

Answer 61

p24

Question 62

Specific diagnostic test to hepatitis C

Answer 62

Anti-HCV

Question 63

Serologic marker during window period for hepatitis B

Answer 63

Anti-HBc

Question 64

Rapid method for diagnosis of blistering disorders such as herpes simplex (multinucleated giant cells)

Answer 64

Tzanck smear

Question 65

Toxigenicity test: C. diphtheriae

Answer 65

Elek’s test

Question 66

Test for LGV (C. trachomatis)

Answer 66

Frei’s test

Question 67

Test for toxoplasmosis

Answer 67

Sabin-Feldman

Question 68

Fungal organism diagnosed by a serum antigen detection test as opposed to serum antibody detection assay

Answer 68

Cryptococcus

Question 69

Measures production of parasitic lactate dehydrogenase

Answer 69

OptiMal Malaria test (pLDH: produced by all four species)

Question 70

Histidine Rich Protein 2Agb (HRP-2 Ag) test for P. falciparum

Answer 70

MalaQuick Standby Malaria test