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ISBB > Serology > Flashcards

Serology Flashcards

1
Q

Normal saline solution isotonic concentration

A

0.85% NaCl

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2
Q

Alkaline phosphatase color and source

A

Yellow (E. coli)

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3
Q

Peroxidase color and source

A

Green (horseradish)

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4
Q

Fluorescence color of FITC

A

Green fluorescence

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5
Q

Half life of 125 ^ I

A

60 days

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6
Q

RIA that can be used to measure total IgE (competitive)

A

Radioimmunosorbent test (RIST)

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7
Q

RIA used to measure allergen specific IgE (noncompetitive)

A

Radioallergosorbent test (RAST)

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8
Q

RIA noncompetitive (sandwich assay)

A

Immunoradiometric Assay (IRMA)

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9
Q

Structure of DNA

A

Helical, double-stranded

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10
Q

Purine bases

A

Adenine, Guanine

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11
Q

Pyrimidine bases (DNA)

A

Cytosine and Thymine

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12
Q

Cofactor to polymerase

A

MgCl2 (Mg)

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13
Q

3 steps of PCR

A
  1. Denaturation (95 deg C)
  2. Annealing (52 deg C)
  3. Extension or Elongation or Synthesis (72 deg C)
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14
Q 
Molecular Blotting Techniques
Southern blot -
Northern Blot - 
Western Blot -
Eastern Blot -
A

SB - DNA
NB - RNA
WB - protein and glycoprotein
EB - lipids and glycolipids

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15
Q

Source of complement for complement related testing

A

Guinea pig

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16
Q

Indicator cell of complement related tests

A

Sensitized sheep RBCs

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17
Q

First diagnostic blood test for syphilis

A

Wasserman test

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18
Q

Interpretation of results for TPI

A

Positive > 50% immobilized
Negative <20% immobilized
Doubtful 20-50% immobilized

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19
Q

Rbc control for neutralization/inhibition should show

A

No hemolysis

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20
Q

SLO control for neutralization test should show

A

Hemolysis

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21
Q

Most common source of alkaline phosphatase in immunoassays

A

Calf intestine

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22
Q

Natural clumping / fleecy mass when a suspension of antigens and antibodies is agitated

A

Flocculation

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23
Q

Net flow of hydrated ions in one direction when the electric field is applied

A

Endo-osmosis or electro-osmosis

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24
Q

Which complement has the lowest concentration of all complement proteins

A

Factor D

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25
Q

Main function is to bind to C8 and prevent insertion of C9 into host cell membranes

A

MIRL (CD59)

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26
Q

Another name for CD 21 (receptor for Epstein Barr Virus)

A

CR2

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27
Q

Another name for CR3 (adhesion and increased activity of phagocytic cells

A

CD11b/CD18

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28
Q

Represents the classes of immunoglobulins

A

Isotype

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29
Q

Direct antiglobulin test can detect in vivo sensitization of red cells by (?)

A

IgG or C3d

30
Q

Serological test used to determine the presence of immunity to Corynebacterium diphtheriae

A

Shick’s test

31
Q

Serological test used to identify susceptibility to scarlet fever

A

Dick’s test

32
Q

Antigen associated with acetylcholinesterase

A

Cartwright

33
Q

What is used in the assay for AH50 test for the overall deficiencies of the alternative parhway

A

Rabbit

34
Q

Proponent for phagocytosis

A

Elie Metchnikoff

35
Q

Proponent for complement

A

Jules Bordet

36
Q

Proponent for monoclonal Ab

A

George Kohler

And Cesar Milstein

37
Q

Proponent for Ab diversity

A

Susumo Tonegawa

38
Q

Inadequate washing in enzyme immunoassays

A

Causes false positive results

39
Q

Which is more sensitive (homogenous or heterogeneous assays)

A

Heterogenous is more sensitive than homogeneous

40
Q

Fluorescence is the property of some compounds to absorb light of one wavelength and emit light of _____

A

Longer wavelength

41
Q

What antigen serves as the receptor for the attachment of the peptic-ulcer causing bacteria (H. pylori) to the gastric mucosa

A

Lewis antigen

42
Q

Responsible for the slapped cheek appearance of parvovirus B19

A

P blood group system

43
Q

Known as CALLA (common ALL antigen)

A

CD10

44
Q

Known as Leukocyte Common Antigen (LCA)

A

CD45

45
Q

Most common phenotype in the black population for Rh

A

Dce

46
Q

Forssman antibodies (?) guinea pig

A

Forssman antibodies absorbed by guinea pig

47
Q

Frequency of the Kell phenotype

A

K-k+ > K+k+ > K+ k-

48
Q

Form of immune complex type of hypersensitivity reaction that results from build up of antibodies to the animal serum used in some passive immunization

A

Serum sickness

49
Q

Minimum number of WBC count in a leukopoor platelet preparation

A

<8.3 x 10^5

50
Q

Minimum wbc count of pooled platelets

A

5.0 x 10^6

51
Q

(+) DAT
(-) antibody screen
Is because of

A

Complement coating rbcs

Drug dependent ab coating red cells

52
Q

Most warm autoantibodies are directed against

A

Rh system

53
Q

Most common type or transfusion reaction

A

Febrile nonhemolytic transfusion reaction

54
Q

Ab of which blood group is most often associated with an anamnestic response

A

ABO

55
Q

Records of transfusion reactions should be kept ___

A

Indefinitely

56
Q

Examples of reverse passive agglutination

A

CRP

RF

57
Q

IgM that directed fo the Fc portion of the IgG

A

Rhematoid Factor

58
Q

Post thaw storage of cryoprecipitate

A

Room temperature, transfuses within 6 hrs

If pooled - 4 hrs

59
Q

Minimum number of WBC count in leukopoor RBC pack

A

<5.0 x10^6

60
Q

Confirmation of heterophile antibody for infectious mononucleosis would be:

A

Agglutination of sheep cells after incubation with guinea pig cells

No agglutination of sheep cells after incubation with beef cells

61
Q

Ab to which Ag is first detected in HIV infection

A

p24

62
Q

Specific diagnostic test to hepatitis C

A

Anti-HCV

63
Q

Serologic marker during window period for hepatitis B

A

Anti-HBc

64
Q

Rapid method for diagnosis of blistering disorders such as herpes simplex (multinucleated giant cells)

A

Tzanck smear

65
Q

Toxigenicity test: C. diphtheriae

A

Elek’s test

66
Q

Test for LGV (C. trachomatis)

A

Frei’s test

67
Q

Test for toxoplasmosis

A

Sabin-Feldman

68
Q

Fungal organism diagnosed by a serum antigen detection test as opposed to serum antibody detection assay

A

Cryptococcus

69
Q

Measures production of parasitic lactate dehydrogenase

A

OptiMal Malaria test (pLDH: produced by all four species)

70
Q

Histidine Rich Protein 2Agb (HRP-2 Ag) test for P. falciparum

A

MalaQuick Standby Malaria test

ISBB (3 decks)

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