serine protease mechanism

Question 1

Histidine acts as a base to extract the proton from the OH group of Ser, inactivating O of OH group

Answer 1

Step 1

Question 2

formation of covalent linkage from OH group of Ser to carbonyl C of peptide bond to be cleaved in substrate

Answer 2

Step 2

Question 3

His act as acid to donate H to amine group of peptide bond to be cleaved, cutting substrate peptide in 2

Answer 3

Step 3

Question 4

His acts as base to extract H from H2O, activate O of molecule

Answer 4

Step 4

Question 5

Activated water cule attack point of covalent linkage between enzyme and substrate

Answer 5

Step 5

Question 6

His act as acid to donate H to reform OH group of Ser

Answer 6

Step 6