seminar 2: Daniell Flashcards

1
Q

what is the pessimistic and optimistic prediction by 2050 for the rise in human pop size?

A

10bn

plateau at 8bn

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2
Q

are the cereal production and human pop size lines parallel and what does this mean?

A

no
per person on the planet there is less food
- higher demand for increased yield

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3
Q

the Haber-Bosch process has been industrialised within the last century

  • what is it?
  • what % of the worlds energy is spent making ammonia from this process
A
  • artificial N fixation process and the main industrial procedure for ammonia production today, splits 3 stable N bonds to form reactive N
  • 2%
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4
Q

per unit of food production what is happening to use of N, particularly as fertiliser?

A

using more and more reactive N

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5
Q

what did steffen et al estimate and what were 3 things discovered?

A

safe operating space for a range of systems

  • direct agricultural involvement
  • N cycle beyond its limit so high risk
  • P cycle beyond its limit and may run out
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6
Q

what does it mean we cant continue to do because the N cycle is beyond its limit?

A

cant put as much reactive N into atmosphere mainly from fertiliser as it causes eutrophication and GHG emission

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7
Q

according to 2016 UK estimates what % is due to agriculture and why is this an underestimate?

A

10%

does not include fertiliser production

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8
Q

in terms of GHG emissions from agriculture how does nitrous oxide compare to CO2?
- what is the main source for each of the gases?

A

N2O 3 times more potent than CO2

  • CO2: fuel for agriculture
  • methane: ruminants
  • N20 from fertilised field and leakage from N cycle
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9
Q

when looking at the C footprint for malt production what % GHG emissions is from

  • fuel
  • N20 from soil
  • N fertilsier
A
  • 17%
  • 35%
  • 48%
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10
Q

from an EPA give 3 points on the nitric and adipic acid production industrial process for reducing emissions

A
  • major source N20
  • all processes involved have costs
  • if all processes in play can save 79% GHG emissions
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11
Q

from an EPA give 4 points on the non rice croplands for reducing emissions

A
  • no costs to reducing emissions such as if tillage of fields
  • less fertiliser, less GHG emission
  • sometimes increased fertiliser can reduce emissions
  • if all processes in play can save only 10%
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12
Q

what does increasing N do to grain yield and why only until a point?

A

increases

saturation occurs

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13
Q

where is the EU on the yield response curve?

A

not adding N we need for optimum yield but are adding max fertiliser can for economic yield
- cost if reduce N

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14
Q

where is China on the yield response curve?

A

further along than EU as adding lots of fertilizer as is cheap but means poor N use efficiency and lots of GHG emissions and leaching damage
- gain if reduce N

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15
Q

where is Brazil on the yield response curve?

A

adding low levels of N

- if add more N can cut GHG emissions

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16
Q

in the N cycle is there competition?

A

yes for compounds in the system such as ammonia which is competed for by plants, microbes and nitrifiers

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17
Q

what are the stages of denitrification?

A

NO3 -> NO2 -> NO -> N20 -> N2

18
Q

why is N fixation highly regulated?

A

very expensive so not fixed unless needed to such as in N limiting systems, for example legumes do this

19
Q

what is soil made up of?

A

bacteria and aggregates surrounded by water film

20
Q

small pores are often low in water, what will bacteria here do?

A

be denitrifying to maintain respiration using nitrogen oxides and nitrates as a different electron receptor to O2

21
Q

in the soil and plant system why is there competition for nitrate?

A

plant uses it for assimilation

bacteria and fungi use it to maintain respiration

22
Q

in the plant microbe interaction what does the plant do? (4)

A
  • physically alters soil environment
  • chemically alters by releasing nutrients and C
  • releases signalling compounds that initiate plant microbe symbiosis
  • produce antimicrobial compounds that interfere with quorum sensing and inhibit microbial activity
23
Q

what are some issues that may arise from plant microbe interactions? (4)

A
  • plant growth limited by nutrient availability and ammonia is used by microbes
  • roots often outcompeted for N by microbes
  • microbial transformations of N limit availability of N
  • nitrifying bacteria convert ammonia to nitrate leading to loss of N
24
Q

in a fertilised system what is there a flush of after it rains?

A

nitrous oxide

25
Q

why was barley used as the plant model ?

A

small, diploid- can link results easily

26
Q

what was the experimental procedure involving barley when looking at denitrification? (4)

A
  • barley cultivars in pots
  • gas measurement taken to see how much nitrous oxide released from soil
  • screen to identify emission variation
  • assessed soil condition effects
27
Q

the plant is the C source in the system so what happens if no plant?

A

little respiration

28
Q

what was nitrous oxide emission rate like in the barley cultivars?

A

varied between

29
Q

in the follow up experiment:

  • how many cultivars
  • the watering treatments
  • the sampling
A
  • 4
  • 70% water filled pore spaces (aerobic), 70,90,70 fluctuating, 90% anaerobic
  • gas sample, plant parameters, microbial pop
30
Q

in the follow up what was found?

A
  • difference between cultivars as to when to stop exploring soil for nutrients
  • little nitrous oxide emission at 90%
31
Q

what was the exudate analysis set up? (6)

A
  • separation of exudate effects
  • no plant but same plotting system
  • artificial exudate injected 0-3 mg C per pot daily for a week
  • watered to 50%, 70%, 90%
  • acetylene used to asses emission
  • assessed community dynamics
32
Q

what does acetylene do to emission when is used?

A

stabilises it

last denitrification step blocked so massive reduction in emission variation and more nitrous oxide emission

33
Q

when a community is fed C what happens to it?

A

grows

34
Q

what 2 genes encode nitrate reductase and what do they contain?

A

NirK (copper)

NirS (iron)

35
Q

what forms with NirS?

A

anaerobic community

36
Q

what are the 2 markers breaking down nitrous oxide?

A

clade i, clade ii

37
Q

what was found when 4 cultivars of barley exudates were collected and analysed?

A

no effect when root exudate C was added to soil

38
Q

what can root exudates moderate?

A

N cycling and drive soil activity

39
Q

where is there significant variation?

A

N2O emission and nitrification

40
Q

what are the effects?

A

indirect and on community structure

due to quantity and quality

41
Q

why is soil condition critical?

A

if high N and low C - competition for C

same the other way round

42
Q

what is the aim in the plants produced?

A

those that use more of the N and release less N as GHG