Semen Collection Techniques Flashcards
Performed by EE or through the use of AV
Bovine
Digital massage of _______ through the rectum produces ________, semen of variable quality and is infrequently employed
Bovine:
Digital massage of accessory glands through the rectum produces erratic responses, semen of variable quality and is infrequently employed
Bovine:
Semen collected from EE are usually___________________
greater in volume,
higher pH,
lower concentration of
spermatozoa and fructose per mL of semen
Bovine:
In EE, probes with three ventrally located electrodes are superior to the four-electrode model due to ________________
a more focused stimulation of the reproductive tract, less
muscular rigidity, and a firmer foot placement
Bulls are restrained in a chute, then the probe is
lubricated, inserted into the evacuated rectum and an
assistant holds the yoke to prevent the bull from
expelling the probe
EE in bulls
EE in bulls:
Semen is maintained at_____
Semen is maintained at 30-35 deg. C
device should be of proper length, and semen should be ejaculated near the directing cone rather than in the liner to avoid sperm death by high temperature
AV in Bovine
AV in Bovine:
If additional ejaculates are desired, the bull must be
rested for _______before repeating with a new
AV
10 to 15 mins
AV is preferred, as EE is not tolerated
Equines (Stallions)
Rigid AV uses rubber or disposable liners and collecting cones, whereas cones are an integral part of the soft AV
Equine
Equine:
Two types of AV are used
one with a more rigid outer
casing, and one with two layers of soft rubber with a small harness for carrying support
Equine:
Recommended water temperature is ________
between 45-50 deg. Celsius
Sterile plastic bottles can be used as ________, filters can be added to remove the gel fraction from the ejaculate
Equine:
collection vials
A __________ which the stallion is accustomed to is used in semen collection
mounting mare / mounting phantom
The mare is _________ for sanitation and prevent penile abrasions during collection
prepared by clipping or wrapping the tail
Equine:
______ is thoroughly washed and dried before
mounting
Perineal area
Stallion is teased to _______, then the penis is washed and the ________removed
to produce erection,
smegma removed
Equine:
___________occurs after
ejaculation and continues until dismount
Flagging (up and down motion of stallion’s tail)
As the stallion moves toward the mare, the penis is guided towards the AV by
grasping the penile shaft
Equine:
Mare’s head is turned partially toward the stallion as he dismounts to prevent __________
kicking and injury
Semen is collected via EE (common) or AV
Ovine
Ovine:
In EE, a __________ is inserted into the rectum of the ram and semen collection is similar to the bull
three-electrode rectal probe
Ovine:
In EE, two approaches may be
1) ram remains standing
supported by a crate and removable boards to restrict
movement, or
2) lateral recumbency, which facilitates easier exteriorization of penis but requires physical restraint
In AV, an immobile, non-estrus ewe is used, restrained in a
head catch and side restraining bars
Ovine:
Ideal temperature for semen collection is _________
between 40-45 deg. Celsius
In AV, _________is used, restrained in a head catch and side restraining bars
an immobile, non-estrus ewe
Ideal temperature for semen collection is between _______
between 40-45 deg. Celsius
EE is more preferable in the buck for semen evaluation, AV is preferable for semen for AI
Caprine semen collection techniques
EE is done via standing or lateral recumbency similar to rams (three-electrode, 3-4 secs followed by a 2-3 secs rest phase, at 0-60 mV)
Caprine
Caprine:
In AV, an untrained buck requires ________ though ________may only require a non-estrus doe
In AV, an untrained buck requires a doe in estrus, though trained ones may only require a non-estrus doe
Uses either EE, direct manipulation (DM, more preferred) via fingers of a gloved hand, or with an AV
Porcine
Porcine:
AV requires ___________
teaser sow, estrous sow or phantom
Porcine:
_______ are recommended since latex gloves are spermicidal
Vinyl gloves
Porcine:
AV for collection is similar with other species, with the
interior of the casing between ________and should collect approx.__________ of semen
45-50 deg. Celsius and should collect approx. 400-500 mL of semen
Porcine:
Collection by EE requires ___________
general anesthesia (thiopental)
Porcine:
EE requires an evacuated rectum, with the probe having_______
circular rather than longitudinal electrodes
Porcine:
Power is administered for______, followed by a rest
phase of _______
Power is administered for 5-7 secs., followed by a rest
phase of 5-10 secs
Porcine:
The first collection is sperm-poor, and is discarded, followed by the sperm rich fraction intermixed with the gel portion
True
