Insemination Procedures

Question 1

Bovine:
Semen is usually packed in_______ plastic
french straws

Answer 1

0.25 or 0.5 mL

Question 2

Bovine:
Semen is stored in ________

Answer 2

liquid nitrogen tanks

Question 3

Bovine:
Straws are thawed rapidly in a _______ for
________, then dried

Answer 3

35°C water bath for 30-60 seconds, then dried

Question 4

Bovine:
Straws are loaded into an_________ after the
sealed tip has been removed

Answer 4

insemination gun

Question 5

Bovine:
Semen should not be exposed during _________

Answer 5

transport and before insertion

Question 6

Bovine:
Rigid pipette attached to a small syringe is used for
___________________

Answer 6

AI semen contained in ampules

Question 7

Bovine:
Insemination procedure involves _________________________________

Answer 7

passing the AI gun through the cow’s vagina, while manipulating the cervix through rectal palpation with one hand

Question 8

Bovine:
Once the pipette reaches the external cervical os, the AI gun is pushed through the sanitary sheath and guided past the _________________

Answer 8

3 to 5 cervical rings

Question 9

Bovine:
Site of deposition is at the level of _______________

Answer 9

the internal cervical os

Question 10

Equine:
Mare is prepared for insemination by _______________

Answer 10

securing her in a set of stocks

Question 11

Equine:
Tail is wrapped and tied out of the way or held by an assistant

Answer 11

true

Question 12

Equine:
Semen is aspirated into a___________________

Answer 12

non-spermicidal syringe

Question 13

Equine:
Perineal area is washed and rinsed with water (how many washes?)

Answer 13

3-5 washes

Question 14

Equine:
Inseminator puts on a ____________, applies sterile lubricant to the glove and passes the insemination pipette through the ________ and guides the tip of the pipette through the ___________

Answer 14

Inseminator puts on a sterile plastic shoulder-length glove, applies sterile lubricant to the glove and passes the insemination pipette through the vagina and guides the tip of the pipette through the cervix

Question 15

Ovine:
Most ewes are bred by __________ or ________

Answer 15

natural service with the ewes kept in pastures or in semi- confinement together with rams

Question 16

Ovine:
Insemination is performed in a ______ or placed on a tilt table which elevates the hindquarters

Answer 16

standing ewe

Question 17

Ovine:
Estrus detection is done through a ___________ to identify females in standing estrous

Answer 17

vasectomized teaser male

Question 18

Ovine:
Insemination should be done _________________

Answer 18

12 to 24 hours after onset of estrus

Question 19

Caprine:
Perineal area is cleaned with _____and a_______ is passed into the vagina

Answer 19

water and a lubricated speculum is passed into the vagina

Question 20

Caprine:
Cloudy/turbid mucus indicates ____________

Answer 20

the doe to be in late estrus (optimum time for AI)

Question 21

Caprine:
AI procedure is similar to that done in ________

Answer 21

bulls

Question 22

Porcine:
Normally the sow/gilt is inseminated with ____________ using fresh semen extended to a volume of______________

Answer 22

2 to 4 x 109 motile spermatozoa using fresh semen extended to a volume of 80 to 100 mL

Question 23

Porcine:
Estrus detection is done through a _______ or ___________

Answer 23

teaser boar or other estrus detection method

Question 24

Porcine:
Standing estrus lasts ____ (gilts) or _______ (sows)

Answer 24

24 to 36 hours (gilts), or 30-60 hours (sows)

Question 25

Porcine:
Gilts should be mated ________, then ________ if still on standing heat

Answer 25

12-18 hours after onset of estrus, then 12 hours later if still on standing heat

Question 26

Porcine:
Sows are bred _______________ and _____________

Answer 26

24 hours from onset of estrus and again 12 to 18 hours later

Question 27

Porcine:
Insemination involves a ________, or a __________

Answer 27

spirette, or a disposable pipette with a foam rubber tip

Question 28

Porcine:
When resistance is detected with the _______, it is gently ______________ so that the spiral end advances and locks into the cervix

Answer 28

When resistance is detected with the spirette, it is gently rotated counter clockwise so that the spiral end advances and locks into the cervix

Question 28

Porcine:
Vulva is cleaned with a _______ and the lubricated spirette is inserted along the ____________

Answer 28

with a paper towel and the lubricated spirette is inserted along the dorsal vaginal wall to the external cervical os

Question 29

Porcine:
A pipette will ______________ with manipulation and will lock into the cervix

Answer 29

advance past one or two cervical rings

Question 30

Porcine:
Semen is injected slowly into the ______ with a ______ or ___________

Answer 30

with a collapsible
plastic bag or bottle