Analysis of semen

Question 1

Collected semen is brought to the laboratory, where the container is placed in a _____, _____ or _____ at _____ for temperature stability

Answer 1

Collected semen is brought to the laboratory, where the container is placed in a water bath, incubator, or dri-block heater at 35-37 deg. Celsius for temperature stability

Question 2

______ is examined first, then normal morphology

Answer 2

Motility

Question 3

Semen is stored and handled in an __________ until analysis is completed and processing begins

Answer 3

isothermal environment

Question 4

Estimated through microscopic examination at 40-100x

Answer 4

Motility

Question 5

_____drop of sample is placed on a slide, cover-slipped and evaluated for % motility

Answer 5

5 uL

Question 6

Generally, a sperm motility of at least _______ is considered acceptable, though it depends on whether __________________

Answer 6

30%

minimally acceptable motility standards are considered

Question 7

Done after motility examination, clearly visualized via eosin-nigrosin, India ink, or Wright’s stain dyes

Answer 7

Morphology

Question 8

Morphology:
Abnormalities can be classified as primary ________ or ____________

Answer 8

primary (e.g head shape) or secondary (minor, e.g bent
tails)

Question 9

Satisfactory score for bulls is_______ normal morphology, without distinguishing between types of abnormalities

Answer 9

70% normal morphology

Question 10

________ may also be assess to evaluate capacity of acrosome to
easily disintegrate and affect fertility

Answer 10

Acrosomal activity/activation

Question 11

Head defects

Answer 11

thin head
large
small
double
alongated
amorphous

Question 12

Midpiece defects

Answer 12

bent
irregular
cytoplasmic (droplet?/droplethin?)
thick
asymetric

Question 13

Tail defects

Answer 13

no tail
short
broken
coiled
terminal droplet
double