Semen

Question 1

Semen is an organic fluid, also known as ________, that may contain ________. It is secreted by the ______ and other sexual organs of male or ________
animals and can fertilize female ova.

In humans, seminal fluid contains several components besides spermatozoa:
1.
2.
3.
are elements of seminal fluid which promote the survival of spermatozoa and provide a medium through which they can move or “swim”

Answer 1

seminal fluid
spermatozoa
gonads (sexual glands)
hermaphroditic

proteolytic
other enzymes as well
as fructose

Question 2

Physical or Macroscopic Examination

Normal volume is ________.

The average volume is _____ after 7 days of abstinence.

Amount ______ is abnormal, however, 1.5 ml per ejaculation is considered normal.

Answer 2

1.5-5.0 mL

3.0 mL

<1.5 mL

Question 3

Macroscopic Examination
Motility Test

A drop of freshly ejaculated semen (after 6 days of sexual abstinence) is placed on a slide and focused under LPO shifts to HPO.

\_\_\_ of the sperm cells are active in motility and 20% are sluggish or are already dead. 

\_\_\_ are active in motility when slid with semen is left within 6-8 hours. 

\_\_\_ after 12 hours at room temperature.

Answer 3

80%
20%

25%-45%

0%

Question 4

Macroscopic Examination
Morphological Examination

1. Morphology of sperm cells could be studied using ________ Method,
2. Wherein a smear is prepared on a glass slide,
3. Fixed with ________,
4. washed with water
5. Decolorized with _______ and
6. stained it with ______, air dried, and focused on oil immersion.

Answer 4

Micgugan Carpenter
95% ethanol
95% ethyl alcohol
Rose Bengal or Eosin

Question 5

REVIEW SPERM DEFECTS

Question 6

CHEMICAL TEST
Florence Test

1. One drop of seminal fluid was placed in a glass slide, and a drop of Florence reagent was added.
2. The slide was then covered with a cover slip and focused under the LPO and the HPO.
3. The presence of
   a.
   b.
   confirms the presence of a seminal stain.

Florence reagent: prepared by dissolving of _______, __________ in ____ mL distilled water.

Answer 6

a. dark-rhombic or needle-shaped crystals
b. the choline periodide

1.65 grams KI
2.54 grams Iodine
30

Question 7

CHEMICAL TEST
Barbeiro’s Test

1. 20 drops of the seminal fluid were placed in a test tube, then was centrifuged.
2. The clear liquid was pipetted off and Barbeiro reagent was placed. The presence of slender, yellow, needle-like crystals which are the ____________, confirmed the presence of seminal fluid.

Barbeiro’s Reagent: _______________

Answer 7

sperminepicrate crystals

aqueous picric acid solution or alcoholic picric acid solution.

Question 8

CHEMICAL TEST
Test for the Presence of Fructose

Fructose is the major sugar of semen and diminished levels parallel _______ deficiency.

Normal values by biochemical analysis is __mg/__mL

Perform 3 tests for both fructose and semen in a separate test tube and note the reactions. Compare results.
1.
2.
3.

Answer 8

androgen

315mg/100ml

1. Molisch Test:
2. Anthrone Test:
3. Test for ketoses: Seliwanoff’s Test

Question 9

CHEMICAL TEST
Test for the Presence of Fructose
1. Molisch Test:

1. To 2 test tubes, put 10 drops each of seminal fluid and _____
2. Add _____ and mix thoroughly.
3. Incline the test tubes and allow _______ to flow down the sides of the tubes, thus forming a layer of acid beneath the samples.
4. Observe the color at the junction of 2 liquids.

Answer 9

10 drops 5% fructose solution.
2 drops of Molisch reagent
20 drops of concentrated sulfuric acid (H2SO4)

Question 10

CHEMICAL TEST
Test for the Presence of Fructose
2. Anthrone Test:

1. To 2 test tubes, put 10 drops each of the seminal fluid and 5% fructose solution.
2. Then add ________.
3. Shake gently and let the solution stand for __ minutes.
4. If the solution is milky, dilute with ________.

Answer 10

20 drops of 0.2% solution of Antrone
30 min
glacial acetic acid

Question 11

CHEMICAL TEST
Test for the Presence of Fructose
3. Test for ketoses: Seliwanoff’s Test:

1. To 2 test tubes, put 10 drops of seminal fluid and 5% fructose solution.
2. Add 10 drops of Seliwanoff’s reagent.
3. Heat the mixture in water bath until visible color appears. Record your observation.

Question 12

CHEMICAL TEST
Test for other ions in seminal fluid
1. Test for chloride ions:

1. Place 20 drops of seminal fluid in a test tube,
2. add_______, then add ________.
3. Centrifuge.
4. Observe the formation of color ____ ppt. which indicates the presence of _______.

Answer 12

3 drops of dilute nitric acid
20 drops of AgNO3 solution
grayish-white
chloride ions

Question 13

CHEMICAL TEST
Test for other ions in seminal fluid
2. Test for proteins:

a. Place 20 drops of seminal fluid in a test tube, then add\_\_\_\_\_\_\_\_ and add \_\_\_\_\_\_. Observe the color. 
 

b. Place 20 drops of seminal fluid in a test tube, then add \_\_\_\_\_\_. Heat in a boiling water for 10 mins, then allow to cool rapidly in running tap water then add \_\_ drops of freshly prepared \_\_\_\_\_\_\_. Warm gently in water bath and observed the color form.

Answer 13

5 drops of CuSO4 solution
5 drops of dilute NaOH

5 drops of Millon’s reagent
4 drops, 0.1% NaNO2

Question 14

CHEMICAL TEST
Test for other ions in seminal fluid
3. Test for Phosphate

1. Place 20 drops of seminal fluid in a test tube,
2. add ______,
3. then add ________ solution and
4. _________,
5. heat in a boiling water bath for 5 mins, cool, observe.

Answer 14

5 drops of dilute HNO3
20 drops of ammonium molybdate
5 drops of dilute NH4OH

Question 15

CHEMICAL TEST
Test for other ions in seminal fluid
4. Test for Calcium ions

1. To the remaining seminal fluid, place it in a test tube,
2. add _________.
3. Stir, then centrifuge.
4. Observe the color of the precipitate.

Answer 15

20 drops of ammonium oxalate solution