Blood Flashcards
_______ is a type of connective tissue with a liquid matrix and is the most active
transport system of the body. Blood plasma is the most physiologically active tissue of the body.
Blood
The proteins of plasma are diverse and complex, serving multiple functions. The most important are:
1.
2.
3.
- albumin
- globulin
- fibrinogen
A. General Test for Blood
Hemin Test
- Place 1 drop of heparinized blood on a glass slide then add 1 drop of ___________
- Evaporate the mixture over a water bath. Cool and examine under
the microscope. - Draw what you have seen and label.
1 % NaCl in a
glacial acetic acid.
A. General Test for Blood
Benzidine Test
- Prepare dilute blood by mixing 1 drop of blood with 5 mL of distilled water.
- Into 2 mL of the diluted blood, add ___________ and __________.
- Observe the formation of ____or ____color.
Describe the result below.
What is the significance of the formation of blue color? Write the reaction involved
RESULTS
The _______ color formation indicates the presence of ______ in the blood. Hemoglobin reacts with BENZIDINE and HYDROGEN PEROXIDE, resulting in a green to blue color. This reaction demonstrates the peroxidase-like activity of hemoglobin, which oxidizes benzidine in the presence of hydrogen peroxide.
6 mL of 1 % benzidine solution
2 mL of 3 % H2O2 solution
green or blue
RESULTS
green
hemoglobin
Test for Blood Constituents
Filtrate and Residue Prep
- Place 2.5 mL of freshly drawn blood or a defibrinated blood with 25 mL of distilled
water in a small beaker. - Heat to boiling then add 4 drops of 10% acetic acid and continue heating for few more minutes.
- Filter the solution while hot. Set aside the residue for the test for iron and use the filtrate for the following tests.
Test for Blood Constituents
Test for Chlorides
- Acidify 1 mL of filtrate using _________, then
- add _______ solution.
A ___________ indicates the presence of chlorides
RESULTS
No white precipitate formed, indicating the absence of chlorides in the blood filtrate. This means that chloride ions, which would typically react with silver nitrate (AgNO3) to form a white precipitate of _________, were not present in significant amounts.
0.1 M HNO3
2 to 3 drops 5% AgNO3
white precipitate
RESULTS
silver chloride (AgCl)
Test for the Presence of Phosphates
1.Acidify 2 mL of the filtrate with ______ .
2. Add ____________.
The formation of ________ means a positive result.
RESULTS
The _________ confirms the presence of phosphates in the blood filtrate. When phosphates react with ammonium molybdate in an acidic solution, they form __________, which appears as a yellow precipitate.
0.1M HNO3
4 mL of 5% Ammonium
molybdate solution
yellow precipitate
RESULTS
*yellowish jelly-like formation and small amount of yellow precipitate
*ammonium phosphomolybdate
Test for Blood Constituents
Benedict’s Test
- Place 2 mL of the filtrate in a test tube.
- Add ___________.
- Heat the mixture over a water bath and observe the formation of color _______
precipitate.
RESULTS
The formation of color ________ precipitate indicates the presence of reducing sugars in the blood filtrate.
Reducing sugars react with Benedict’s reagent under heat to form _______, a brick-red solid, showing that ________ are present.
4 drops of Benedict’s reagent
brick red
RESULTS
brick-red
copper(I) oxide
reducing sugars
Test for Blood Constituents
Test for Iron
- Heat the residue that was set aside in an evaporating dish until all the organic
matter has completely burned out. - Cool then add_________solution.
- To the dissolved material add _________ solution and take note of the color produced.
RESULTS
The _______ observed after burning the residue and treating it with hydrochloric acid and potassium thiocyanate (KCNS) indicates the presence of _____. Iron ions in the residue react with KCNS to form iron thiocyanate complexes, which produce a greenish tint.
2 to 3 mL of 10% HCl (Hydrocgloric acid)
3 to 4 drops of 5% KCNS (Potassium Thiocyanate)
RESULTS
green color
iron
______ is the most physiologically active tissue of the body.
Blood plasma
