SEDIMENT TECHNIQUE

Question 1

What is the most frequently used stain in urinalysis?

A) Gram stain
B) Sternheimer-Malbin stain
C) Wright’s stain
D) Giemsa stain

Answer 1

B) Sternheimer-Malbin stain

Question 2

What are the components of Sternheimer-Malbin stain?

A) Methylene Blue & Eosin Y
B) Crystal Violet & Safranin O
C) Hematoxylin & Eosin
D) Giemsa stain & Wright stain

Answer 2

B) Crystal Violet & Safranin O

Question 3

Which of the following stains is commercially available under the name Sternheimer-Malbin stain?

A) Sedi stain
B) KOVA stain
C) Both A and B
D) Neither A nor B

Answer 3

C) Both A and B

Question 4

What is the primary purpose of the Sternheimer-Malbin stain when used with lipid stains such as Oil Red O, Sudan III, and polarizing microscopy?

A) To identify nucleic acids
B) To confirm the presence of triglycerides, neutral fats, and cholesterol
C) To highlight protein structures
D) To visualize carbohydrates

Answer 4

B) To confirm the presence of triglycerides, neutral fats, and cholesterol

Question 5

What is the primary purpose of Gram stain in microbiology?

A) Differentiating between viral and bacterial infections
B) Identifying fungal species
C) Determining the presence of bacterial casts
D) Assessing the antibiotic susceptibility of bacteria

Answer 5

C) Determining the presence of bacterial casts

Question 6

What are the components of a Gram stain?

Answer 6

* Use of Crystal Violet (a purple dye), iodine (as a mordant), alcohol (as a decolorizer), and Safranin (a counterstain).

Question 7

Which color is associated with Gram-positive bacteria in a Gram stain?

A) Red
B) Violet
C) Green
D) Blue

Answer 7

** B) Violet**

Question 8

Which color does a Gram-negative bacteria stain appear as in a Gram stain?
A) Purple
B) Violet
C) Red
D) Blue

Answer 8

C) Red

Question 9

What is the preferred stain for identifying urinary eosinophils in the case of drug-induced allergic reactions producing inflammation of the renal interstitium?

A) Gram Stain
B) Hansel Stain
C) Wright Stain
D) Ziehl-Neelsen Stain

Answer 9

B) Hansel Stain

Question 10

What components are used in a Hansel stain?

A. Methylene Blue and Eosin Y
B. Crystal Violet and Safranin
C. Gram Stain and Wright Stain
D. Iodine and Alcohol

Answer 10

A. Methylene Blue and Eosin Y

Question 11

What is the primary staining target of Prussian Blue stain?

A) Nuclei

B) Cytoplasm

C) Iron (Hemosiderin granules)

D) Collagen fibers

Answer 11

C) Iron (Hemosiderin granules)

Question 12

Which technique enables the visualization of naturally fluorescent microorganisms or those stained by a fluorescent dye, including labeled antigens and antibodies?

A) Immunohistochemistry
B) Fluorescence Microscopy
C) Electron Microscopy
D) Immunofluorescence Assay

Answer 12

B) Fluorescence Microscopy

Question 13

Which microscopy technique provides three-dimensional imaging and allows layer-by-layer visualization of a specimen?

A) Fluorescence microscopy
B) Bright-field microscopy
C) Phase-contrast microscopy
D) Interference contrast microscopy

Answer 13

D) Interference contrast microscopy