CENTRIFUGATION

Question 1

What is a common variation in centrifugation time and speed for urine sedimentation in different laboratories?

A) 5 minutes @ 400 RCF
B) 10 minutes @ 600 RCF
C) 3 minutes @ 300 RCF
D) 7 minutes @ 500 RCF

Answer 1

A) 5 minutes @ 400 RCF

Question 2

What volumes are frequently used in centrifugation for achieving a uniform amount of urine and sediment?

A) 2 mL and 3 mL
B) 0.1 mL and 0.2 mL
C) 0.5 mL and 1 mL
D) 5 mL and 10 mL

Answer 2

C) 0.5 mL and 1 mL

Question 3

What is the recommended volume for the conventional glass slide method in centrifugation?

A) 10 uL (0.01 mL)

B) 15 uL (0.015 mL)

C) 20 uL (0.02 mL)

D) 25 uL (0.025 mL)

Answer 3

C) 20 uL (0.02 mL)

Question 4

What is the primary purpose of the LPO (Low Power Objective) in centrifugation during the analysis of sediment?

A) To enhance the brightness for better visibility of small particles
B) To detect casts and ascertain the general composition of the sediment
C) To increase the speed of centrifugation
D) To isolate big epithelial cells

Answer 4

B) To detect casts and ascertain the general composition of the sediment

Question 5

Which of the following techniques is commonly used for the identification of urinary sediments in the context of HPO (Human Phenotype Ontology)?

A) Chromatography
B) Centrifugation
C) Spectroscopy
D) PCR (Polymerase Chain Reaction)

Answer 5

B) Centrifugation

Question 6

In the conventional glass method of centrifugation, where does the cast tend to locate?

A) In the center of the coverslip
B) Near the middle of the coverslip
C) Near the edges of the coverslip
D) Randomly across the coverslip

Answer 6

C) Near the edges of the coverslip