Section 5: Energy transfers in and between organisms Flashcards
Which wavelengths of light are best absorbed by chlorophyll
Orange and blue
How does the structure of the chloroplast enable effieciently photosynthesis.
The structure of the chloroplast enables efficient photosynthesis by:
1. Thylakoid membranes: Provide a large surface area for the attachment of chlorophyll, enzymes, and electron carriers involved in the light-dependent reactions.
2. Stroma: Contains enzymes for the light-independent reactions (Calvin cycle) and surrounds the thylakoids to allow easy diffusion of products from the light-dependent reactions.
What stage of photosynthesis occurs in the grana
Light dependent reaction
Name 2 products of the LDR that are needed in the LIR and what is the other waste product
ATP and NADPH used
O2 waste ish
What happens when chlorophyll pigments absorb light and what is this process called
Excited electrons and they move out of PS2 along electron transfer chain called photoionisation
How does light effect water in the LDR
Photolysis energy used to split water into protons oxygen and electrons. electrons goes to PS2
Where do the sub products of photolysis go and what stage of photosynthesis is it
Light dependent reaction
Proton/hydrogen is picked up by NADP to from NADPH used in the LIR
The electron goes to PS2 and is passed along electron transfer chain
The oxygen either used for respiration or diffuses out of the leaf through stomata
What happens to NADP in the LDR
Reduced
attaches to H to from NADPH
What is the process that involves the movement of hydrogen ions across a membrane to generate ATP and what stage of photosynthesis is it
Light dependent reaction
Chemiosmosis
Describe chemiosmosis
Chemiosmosis during the light-dependent reaction (AQA A-Level, 4 marks):
1. Energy from excited electrons, transferred along the electron transport chain in the thylakoid membrane, is used to pump protons (H⁺) into the thylakoid space from the stroma.
2. This creates a high concentration of protons in the thylakoid space, establishing a proton gradient across the thylakoid membrane.
3. ATP synthase: Protons diffuse back into the stroma (as-well as protons from photolysis) through ATP synthase, down the concentration gradient.
4. ATP formation: The movement of protons through ATP synthase provides energy for the synthesis of ATP from ADP and inorganic phosphate (Pi).
Who discovered the reactions of the light-independent reaction stage
Melvin Calvin
What is the role of ribulose bisphosphate?
Carbon fixation: combines to CO2 catalysed by enzyme rubisco to eventually release organic molecules
Give three reasons why ATP is required in the light-independent reactions
ATP is required in the light-independent reactions for:
1. Reduction of GP: Provides energy to reduce glycerate-3-phosphate (GP) into triose phosphate (TP).
2. Regeneration of RuBP: Supplies energy for the regeneration of ribulose bisphosphate (RuBP) from TP.
3. Synthesis of organic molecules: Provides energy for the formation of glucose and other carbohydrates.
What is the role of NADPH in the light independent reactions
- Reduction of GP: NADPH reduces GP into TP
- Provides hydrogen: Supplies the hydrogen needed for the reduction process.
What would happen to the level of GP if the lights were turned off?
- GP levels would increase: As RuBP continues to combine with CO₂, forming more GP.
- No reduction of GP: Without light, no NADPH or ATP is produced in the light-dependent reactions, so GP cannot be reduced to TP.
Name three possible products that might be synthesised as a result of the light-independent reactions?
- Glucose (or other carbohydrates).
- Amino acids.
- Lipids.
Explain why the rate of photosynthesis will not increase indefinitely if the light intensity continues to be increased
- Limiting factors: Other factors, such as CO₂ concentration or temperature, become limiting.
- Saturation point: Light-dependent reactions reach a maximum rate as all photosystems are saturated.
How many carbon molecules does these molecules have:
RuBP
GP
TP
CO2
RuBP = 5C
GP = 3C
TP = 3C
CO2 = 1C
Describe how NADP os reduced in the light-dependent reaction
- Electrons: NADP is reduced by electrons from the electron transport chain.
- Protons: NADP also combines with protons (H⁺) from the photolysis of water (after it has gone through ATP synthase) to form reduced NADP (NADPH).
Describe the part played by reduced NADP in the light-independent reaction
- Reduction of GP: Reduced NADP (NADPH) donates electrons and hydrogen ions to reduce GP to TP
- provides energy from hydrolysis reaction of NADPH splitting
Describe 2 features of an ATP molecule which make it a useful source of energy
- Small and soluble: ATP is small and soluble, so it can easily move within cells to where energy is needed.
- Instant release of energy: ATP releases energy quickly when one phosphate group is removed, making it efficient for cellular processes.
- Quickly regenerated: ATP can be easily converted back from ADP and Pi, providing a constant supply of energy.
Write a simple equation summarising reaction involving CO2 GP and RuBP
RuBP + CO2 ——-> 2 GP
Catalysed by Rubisco enzyme
What are the three stages in the light dependent reaction?
Photoionisation: light energy excited electrons they move out of chlorophyll. Chlorophyll has been ionised by light
Photolysis: Light energy absorbed in photo system 2 splits water into protons hydrogen and electron
Chemiosmosis: Electron transfer chain provide energy for Protons to move into thylakoid space which are then pumped back into stroma along with protons from photolysis through ATP synthase release ATP and reduce NADP
Where in a cell is the enzyme rubisco found
Stroma of a chloroplasts
What substance combines with CO2 in a chloroplast
RuBP
Name the four stages of Aerobic respiration
- Glycolysis
- Link reaction
- Krebs cycle
- Oxidative phosphorylation
What is glycolysis?
Glucose (6C) phosphorylated to hexose bisphophate
hexose bisphosphate split into 2TP
Oxidation of TP to produce 2 pyruvates
the production of ATP
What is the link reaction summarised equation
pyruvate + NAD +coA ——> acetyl CoA + reduced NAD + CO2
What is the overall yeild from glycolysis
2 ATP
2 reduced NADP
2 molecules of pyruvate
Describe the Krebs cycle
- Acetyl-CoA combines with 4 carbon molecule to form a 6 carbon molecule
- Series of decarboxylation and dehydrogenation reactions occur.
- CO₂ is released (two molecules per cycle).
- Reduced NAD (three molecules) and reduced FAD (one molecule) are produced.
- ATP is produced via substrate-level phosphorylation.
- 4 carbon molecule is regenerated.