Section 3: Classification Flashcards
1
Q
Linnaeus
A
2 Kingdoms
2
Q
Woese
A
5, then 6 kingdoms: Eubacteria Archaebacteria Protista Fungi Plantae Animalia
3
Q
3 domain system
A
- due to DNA comparison
- Bacteria, Archae, Eukarya
4
Q
Bacterial Nomenclature
A
[Kevin's poop constantly over flows GSS] Kingdom Phylum Class Order Family Genus (Escherichia) Species (E. Coli) Strain (E. Coli 0157:H7)
5
Q
Bacterial Identification (8 points)
A
Morphological Characteristics Biochemical testing Molecular taxonomy Diagnostic molecular pathology Polymerase Chain Reaction Immunological tests Bacteriophage typing Antibiotic sensitivity testing
6
Q
Morphological Characteristics
A
colony characteristics cell shape presence of capsule, endospore staining: G+//G- or neither locomotion: flagella + arrangement ?
7
Q
Biochemical testing
A
metabolic end products
utilization of different substrates (ie. sugars)
acid from fermentation changes pH
hemolysis of RBC’s
8
Q
Molecular Taxonomy
A
size of genome G+C content nucleic acid similarity protein similarity -phylogenetic trees indicate similarity
9
Q
Diagnostic molecular pathology
A
in vivo hybridization
10
Q
Polymerase Chain Reaction (PCR)
A
PCR amplifies/makes millions of copies for easier detection
11
Q
Immunological tests
A
test blood serum for antibodies produced in response to specific bacteria
12
Q
Bacteriophage typing
A
bacteriophage identification links to bacteria//host involved
13
Q
antibiotic sensitivity testing
A
testing subject for susceptibility to identify microbe involved
