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Biology > Section 1 - Biological molecules: 2. Nucleic acids > Flashcards

Section 1 - Biological molecules: 2. Nucleic acids Flashcards

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1
Q

What is a nucleic acid

A

A polymer of nucleotides which store genetic information in biological systems (ie. DNA and RNA)

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2
Q

What is the structure of a nucleotide (3 components)

A
  • Pentose sugar (with 5 carbon atoms)
  • A phosphate group
  • A nitrogen-containing organic base

Components covalently bonded together in condensation reactions

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3
Q

What is the name of the bond between nucleotides in a polynucleotide

A

Phosphodiester bonds

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4
Q

What are the 4 bases in DNA

A
  • Adenine
  • Thymine
  • Cytosine
  • Guanine
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5
Q

What are the 4 bases in RNA

A
  • Adenine
  • Uracil
  • Cytosine
  • Guanine
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6
Q

What are the complementary base pairs in the double stranded helix of DNA

A

Adenine bonds with Thymine (double hydrogen bond)

Cytosine bonds with Guanine (triple hydrogen bond)

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7
Q

How does the structure of DNA relate to it’s function

A
  • Stable (phosphodiester backbone and hydrogen bonds): allows genetic info to be passed on without change
  • Two strands joined by hydrogen bonds: allows for separation during replication
  • Large molecule: hold more genetic info
  • Helix structure: protects the bases from being corrupted by outside forces or chemicals
  • Complementary base pairing: allows for the formation of m-RNA
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8
Q

What were the stages of discovering DNA

A
  • 1869: Johann Friedrich discovered nucleic acids
  • 1919: Phoebus Levene described the nucleotides within them
  • 1944: Oswald Avery discovered that DNA passes on genetic info down generations
  • 1950: Erwin Chargaff discovered the complementary base pairing
  • 1950: Maurice Wilkins and Raymond Gosling began to collect x-ray diffraction patterns of DNA
  • 1952: Rosalind Franklin used improved techniques to produce ‘Photograph 51’, and began evaluating the structure
  • 1953: James Watson and Frances Crick published accurate descriptions of the structure of DNA, using Franklin’s work
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9
Q

What was the experiment used to determine the hereditary nature of material in organisms

A
  • Mice injected with living, safe form of bacteria = healthy
  • Mice injected with dead, harmful form of bacteria = healthy
  • Mice injected with both = infected
    This suggests that the dead harmful bacteria can pass on the information needed to make the toxins.

This hereditary material was proven to be DNA, by removing all parts from the living harmful bacteria and adding the individually to living safe bacteria. Only the DNA caused to trait to be passed on.

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10
Q

What was the experiment used to determine that DNA is responsible for passing genetic info down generations.

A

Viruses infect bacteria to produce more viruses
Viruses only contain DNA and proteins, so one of those must be responsible.
The proteins and DNA in the viruses were labelled with different radioactive elements, and the viruses produced from them only showed signs of radioactive DNA

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11
Q

Where is the 3’ carbon atom located on a pentose sugar

A

Attached to the hydroxyl group (bottom left when sugar is oriented upright)

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12
Q

Where is the 5’ carbon atom located on a pentose sugar

A

Attached to the phosphate group (top left when the sugar is oriented upright)

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13
Q

Why can nucleic acids only be synthesised ‘in vivo’

A

They can only be synthesised in the 5’-3’ direction, as DNA polymerase can only attach nucleotides to the OH group on the 3’ carbon atom

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14
Q

What is semi-conservative replication

A

The process of DNA replication where one strand of the double helix is conserved, with a new strand attached to it

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15
Q

Describe the process of semi-conservative DNA replication

A
  • The double helix is unzipped by DNA helicase, forming a replication fork
  • On the leading strand, leaving the fork with the 3’ end first, the enzyme primase forms a small section of bases attached to it, called a primer
  • DNA polymerase binds to this primer, and forms the new strand in the 5’ to 3’ direction as the original strand is passed through in the opposite direction.
  • On the lagging strand, leaving the fork with the 5’ end first, the enzyme primase forms multiple primers between which DNA polymerase forms the new strand in the same direction that the original is being passed out, from the 5’ to 3’ end
  • These sections of strands formed on the lagging strand are called Okazaki fragments
  • The enzyme exonuclease removes all the primers and they are replaced with DNA by DNA polymerase
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16
Q

What is the enzyme that unzips DNA for replication

A

DNA helicase

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17
Q

What is enzyme that forms primers to begin DNA replication

A

Primase

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18
Q

What is the enzyme the forms the new strand of DNA replication

A

DNA polymerase

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19
Q

What is the name of the sections of DNA formed individually on the lagging strand of DNA replication

A

Okazaki fragments

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20
Q

What direction does the leading strand leave the replication fork in DNA replication

A

3’ to 5’

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21
Q

What direction does the lagging strand leave the replication fork in DNA replication

A

5’ to 3’

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22
Q

What is the enzyme that removes the primers after DNA replication

A

Exonuclease

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23
Q

What were the alternative models of DNA replication instead of semi-conservative replication

A
  • Conservative: original DNA remains intact, and complete new copy is formed
  • Dispersive: new and old DNA randomly alternates in both strands
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24
Q

What is the experiment that supports the model of semi-conservative replication (‘the most beautiful experiment’)

A
  • Bacteria grown in 14-N medium
  • Bacteria moved and grown in 15-N medium for many generations and when DNA is extracted and centrifuged, all DNA is the heavy, containing 15-N
  • After then being grown in 14-N medium for one generation, when extracted and centrifuged, all DNA is in the medium weight band, containing both 14-N and 15-N (disproving conservative replication)
  • After 2 generations in 14-N medium, half the DNA was light containing only 14-N, half was medium containing both (disproving dispersive replication)
  • After 3 generations, 75% is light and 25% is medium, proving semi-conservative replication, as there is no heavy containing only 15-N, but the proportion containing the original strands halves each generation
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25
Q

What is the structure of ATP

A

Adenosine Triphosphate:
- Ribose sugar
- Adenine: organic nitrogenous base
- Phosphates: chain of 3 phosphate groups

26
Q

How does ATP store energy

A

Bonds between the phosphates are unstable, so have a low activation energy and release lots of energy when broken by hydrolysis, forming ADP

27
Q

What enzyme catalyses the hydrolysis of ATP into ADP

A

ATP hydrolase

28
Q

How is ATP synthesised

A

A phosphate molecule is added to ADP in a condensation reaction.

29
Q

What are the 3 ways ATP can be synthesised from ADP

A
  • Happens in plants during photosynthesis (photophosphorylation)
  • Happens in plants and animals during respiration (oxidative phosphorylation)
  • Happens in plants and animals when phosphate groups are transferred from donor molecules to ADP (substrate-level phosphorylation)
30
Q

What is photophosphorilation

A

The synthesis of ATP, by adding a phosphate group to ADP in chlorophyll-containing plant cells during photosynthesis.

31
Q

What is oxidative phosphorylation

A

The synthesis of ATP by adding a phosphate group to ADP in plant and animal cells during respiration

32
Q

What is substrate-level phosphorylation

A

The synthesis of ATP, when a phosphate group is donated from one ADP to another, in plant and animal cells

33
Q

What enzyme catalyses the synthesis of ATP

A

ATP synthase

34
Q

Why is ATP required as well as glucose

A
  • ATP is unstable, so can’t store energy like glucose
  • ATP can be broken down easily for immediate energy release, whereas the breakdown of glucose is a longer process
35
Q

Where is ATP made

A

Constantly made in the mitochondria of the cell that it is needed in.

36
Q

What energy requiring processes is ATP used for

A
  • Metabolic processes
  • Movement
  • Active transport
  • Secretion: formation of lysosomes used for the secretion of cell products
  • Activation of molecules: the inorganic phosphate released can phosphorylate other compounds, lowering their activation energy and making them more reactive
37
Q

What are inorganic ions

A

Ions that are not directly associated with a biological molecule

38
Q

Inorganic ions: What are the functions of iron (Fe^2+)

A
  • Forms prosthetic groups in haemoglobin (Haem groups), associating with O2 for transport in the red blood cells
39
Q

Inorganic ions: What are the functions of Sodium (Na^+)

A
  • Sodium-potassium pumps (in co-transport)
  • Important in the nervous system for communication between neurons.
40
Q

Inorganic ions: What are the functions of Hydrogen (H^+)

A
  • Ion pumps used to maintain pH (digestion)
  • Important on respiration and photosynthesis
41
Q

Inorganic ions: What are the functions of phosphate (PO4^3-)

A
  • Used to form phosphodiester bonds
  • Forms part of the sugar-phosphate backbone of DNA and RNA
  • Part of ATP molecule that is removed by hydrolysis to release energy
  • Used to activate molecules by phosphorylation
  • Part of phospholipids within cell membranes
42
Q

Inorganic ions: What are the functions of Potassium (K^+)

A
  • Regulation of the guard cells, opening and closing the stomata
43
Q

What is the structure of a water molecule

A

Two hydrogen atoms are covalently bonded to one oxygen atom

44
Q

Why is water dipolar

A

Although it has no overall charge, the arrangement of the electrons within the molecule mean that the oxygen side is slightly negative and the hydrogen end is slightly positive

45
Q

Why do hydrogen bonds form between water molecules

A

The negative oxygen of one molecule is attracted to the hydrogen of another, resulting in an electrostatic attraction that forms a hydrogen bond

46
Q

What are the main properties of water

A
  • High specific heat capacity
  • High latent heat of vaporisation
  • Cohesive and adhesive nature of the molecules
  • Surface tension
  • Effective solvent
  • Common reactant in metabolism
  • Not compressible
  • Transparent
47
Q

Why does water have a high specific heat capacity

A

The hydrogen bonds between the molecules means that more energy is required to increase their kinetic energy, so increase the temperature

48
Q

What is an example of the benefits of the high specific heat capacity of water

A

Allows for sustained ecosystems in water based environments, as the temperature of the water doesn’t fluctuate as much as the air around it

49
Q

Why does water have a high latent heat of vaporisation

A

Hydrogen bonds between the molecules mean that more energy is required to change form liquid to gas

50
Q

What is an example of a benefit of the high latent heat of vaporisation of water

A

Allows for thermoregulation, as the evaporation of sweat takes in loads of energy, cooling down the skin

51
Q

Why are water molecules cohesive (and adhesive)

A

The polarity of the water molecules causes them to be attracted to each other, and other molecules

52
Q

What is an example of a benefit of the cohesive nature of water molecules

A

Allows for transpiration to occur, as the cohesion of water causes it to flow up the stem (and adhesives as they are attracted to xylem cells as they pass)

53
Q

Why does water have surface tension

A

Hydrogen bonds near the surface allows water droplets to form

54
Q

What is an example of a benefit of the surface tension of water

A

Allows droplets to form, and the spherical shape has the minimum surface area, so takes longer to evaporate, supporting ecosystems that rely on the presence of water

55
Q

Why is water a good solvent

A

All small and charged particles will dissolve in water as they are attracted to the polar molecules

56
Q

What is an example of water being used as a solvent

A
  • Ocean
  • Blood stream
  • Cytoplasm
  • Tissue fluid
  • Cell sap
  • Transpiration flow
57
Q

Why is water commonly used in metabolic reactions

A

Readily available, so is commonly present to be used

58
Q

What is an example of a metabolic reaction that uses water

A
  • Hydrolysis of compounds
  • Photosynthesis
59
Q

Why is water not compressible

A

Sides of the molecules with the same charge will repel if too close, stopping the water from being compressed

60
Q

What is an example of a benefit of water being non-compressible

A
  • Turgid pressure in plants
  • Hydrostatic pressure differences
61
Q

Why is water transparent

A

The constant breaking and forming of hydrogen bonds allows light to pass through the gaps between molecules

62
Q

What is an example of a benefit of water being transparent

A
  • Allows for photosynthesis in underwater plants
  • Vital for eyesight

Biology (21 decks)

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